Clinical performance of multiplex high-risk e6 mrna expression in comparison with hpv dna subtypes for the identification of women at risk of cervical cancer

Chih Ming Ho, Kui You Pan, Yun Yuan Chen, Chia Yen Huang, Yu Li Chen, Shwu Fen Chang

Research output: Contribution to journalArticlepeer-review

Abstract

We compared multiplex E6 messenger ribonucleic acid (mRNA) tests using real-time quantitative reverse transcriptase polymerase chain reactions (PCR) with human papillomavirus (HPV) DNA subtypes using a MY11/GP6+ PCR-based reverse-blot assay to identify cervical intraepithelial neoplasias of grade 2 or worse (CIN2+). In total, 684 women were studied, of whom 377 (55%) were diagnosed with CIN2+ histologically. The specificity of HPV mRNA to predict histological CIN2+ was higher than that of HPV DNA (81.3% vs. 44.2%). The odds ratios (ORs) to predict histological CIN2+ in women with positive for type 16, 18, 31, and 45 E6 mRNA or by HPV DNA detection were 7.1 (95% confidence interval [CI] 3.9-13.1) and 2.5 (95%CI 1.9-3.5), respectively, compared to those with negative for E6 mRNA or HPV DNA. The OR to predict histological CIN2+ in women with a cytological grade

Original languageEnglish
Pages (from-to)1404-1412
Number of pages9
JournalJournal of Medical Virology
Volume87
Issue number8
DOIs
Publication statusPublished - Aug 1 2015

Keywords

  • Cervical intraepithelial neoplasias
  • E6 messenger ribonucleic acid
  • Human papillomavirus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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