Clinical and biological implications of partial tandem duplication of the MLL gene in acute myeloid leukemia without chromosomal abnormalities at 11q23

H. S. Shiah, Y. Y. Kuo, J. L. Tang, S. Y. Huang, M. Yao, W. Tsay, Y. C. Chen, M. H.au Shen Wang D.-T., K. H. Lin, H. F. Tien

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Abstract

The clinical and biological features of acute myeloid leukemia (AML) with 11q23/MLL translocations are well known, but the characteristics of AML with partial tandem duplication of the MLL gene have not been explored comprehensively. In this study, MLL duplication was analyzed, in 81 AML patients without chromosomal abnormalities at 11q23, using Southern blotting, genomic DNA polymerase chain reaction (PCR), reverse-transcription PCR and complementary DNA sequencing. Nine patients showed partial tandem duplication of the MLL gene, including eight (12%) of the 68 with normal karyotype. Seven patients showed fusion of exon 6/exon 2 (e6/e2), one, combination of differentially spliced transcripts e7/e2 and e6/e2, and the remaining one, combination of e8/e2 and e7/e2. Among the patients with normal karyotype, children aged 1 to 15 showed a trend to higher frequency of MLL duplication than other patients (2/5 or 40% vs 6/62 or 10%, P = 0.102). The patients with tandem duplication of the MLL gene had a significantly higher incidence of CD11b expression on leukemic cells than did those without in the subgroup of patients with normal karyotype (75% vs 28%, P = 0.017). There were no significant differences in the expression of lymphoid antigens or other myeloid antigens between the two groups of patients. In adults, the patients with MLL duplication had a shorter median survival time than those without (4.5 months vs 12 months, P = 0.036). In conclusion, partial tandem duplication of the MLL gene is associated with increased expression of CD11b on leukemic blasts and implicates poor prognosis in adult AML patients. The higher frequency of MLL duplication in children older than 1 year, than in other age groups, needs to be confirmed by further studies.

Original languageEnglish
Pages (from-to)196-202
Number of pages7
JournalLeukemia
Volume16
Issue number2
DOIs
Publication statusPublished - Jan 1 2002
Externally publishedYes

Fingerprint

Gene Duplication
Acute Myeloid Leukemia
Chromosome Aberrations
Exons
Karyotype
Antigens
Polymerase Chain Reaction
DNA-Directed DNA Polymerase
Southern Blotting
DNA Sequence Analysis
Reverse Transcription
Complementary DNA
Age Groups

Keywords

  • Acute myeloid leukemia
  • MLL gene
  • Partial tandem duplication

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Clinical and biological implications of partial tandem duplication of the MLL gene in acute myeloid leukemia without chromosomal abnormalities at 11q23. / Shiah, H. S.; Kuo, Y. Y.; Tang, J. L.; Huang, S. Y.; Yao, M.; Tsay, W.; Chen, Y. C.; Wang D.-T., M. H.au Shen; Lin, K. H.; Tien, H. F.

In: Leukemia, Vol. 16, No. 2, 01.01.2002, p. 196-202.

Research output: Contribution to journalArticle

Shiah, H. S. ; Kuo, Y. Y. ; Tang, J. L. ; Huang, S. Y. ; Yao, M. ; Tsay, W. ; Chen, Y. C. ; Wang D.-T., M. H.au Shen ; Lin, K. H. ; Tien, H. F. / Clinical and biological implications of partial tandem duplication of the MLL gene in acute myeloid leukemia without chromosomal abnormalities at 11q23. In: Leukemia. 2002 ; Vol. 16, No. 2. pp. 196-202.
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abstract = "The clinical and biological features of acute myeloid leukemia (AML) with 11q23/MLL translocations are well known, but the characteristics of AML with partial tandem duplication of the MLL gene have not been explored comprehensively. In this study, MLL duplication was analyzed, in 81 AML patients without chromosomal abnormalities at 11q23, using Southern blotting, genomic DNA polymerase chain reaction (PCR), reverse-transcription PCR and complementary DNA sequencing. Nine patients showed partial tandem duplication of the MLL gene, including eight (12{\%}) of the 68 with normal karyotype. Seven patients showed fusion of exon 6/exon 2 (e6/e2), one, combination of differentially spliced transcripts e7/e2 and e6/e2, and the remaining one, combination of e8/e2 and e7/e2. Among the patients with normal karyotype, children aged 1 to 15 showed a trend to higher frequency of MLL duplication than other patients (2/5 or 40{\%} vs 6/62 or 10{\%}, P = 0.102). The patients with tandem duplication of the MLL gene had a significantly higher incidence of CD11b expression on leukemic cells than did those without in the subgroup of patients with normal karyotype (75{\%} vs 28{\%}, P = 0.017). There were no significant differences in the expression of lymphoid antigens or other myeloid antigens between the two groups of patients. In adults, the patients with MLL duplication had a shorter median survival time than those without (4.5 months vs 12 months, P = 0.036). In conclusion, partial tandem duplication of the MLL gene is associated with increased expression of CD11b on leukemic blasts and implicates poor prognosis in adult AML patients. The higher frequency of MLL duplication in children older than 1 year, than in other age groups, needs to be confirmed by further studies.",
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