Cleavage of DNA by mammalian DNA topoisomerase II.

Leroy-Fong Liu, T. C. Rowe, L. Yang, K. M. Tewey, G. L. Chen

Research output: Contribution to journalArticle

465 Citations (Scopus)

Abstract

Using the P4 unknotting assay, DNA topoisomerase II has been purified from several mammalian cells. Similar to prokaryotic DNA gyrase, mammalian DNA topoisomerase II can cleave double-stranded DNA and be trapped as a covalent protein-DNA complex. This cleavage reaction requires protein denaturant treatment of the topoisomerase II-DNA complex and is reversible with respect to salt and temperature. The product after reversal of the cleavage reaction remains supertwisted, suggesting that the two ends of the putatively broken DNA are held tightly by the topoisomerase. Alternatively, the enzyme-DNA interaction is noncovalent, and the covalent linking of topoisomerase to DNA is induced by the protein denaturant. Detailed characterization of the cleavage products has revealed that topoisomerase II cuts DNA with a four-base stagger and is covalently linked to the protruding 5'-phosphoryl ends of each broken DNA strand. Calf thymus DNA topoisomerase II cuts SV40 DNA at multiple and specific sites. However, no sequence homology has been found among the cleavage sites as determined by direct nucleotide-sequencing studies.

Original languageEnglish
Pages (from-to)15365-15370
Number of pages6
JournalJournal of Biological Chemistry
Volume258
Issue number24
Publication statusPublished - Dec 25 1983
Externally publishedYes

Fingerprint

Type II DNA Topoisomerase
DNA Cleavage
DNA
DNA Gyrase
Type I DNA Topoisomerase
Proteins
Sequence Homology
Thymus
Nucleotides
Salts
Assays
Temperature
Cells
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Liu, L-F., Rowe, T. C., Yang, L., Tewey, K. M., & Chen, G. L. (1983). Cleavage of DNA by mammalian DNA topoisomerase II. Journal of Biological Chemistry, 258(24), 15365-15370.

Cleavage of DNA by mammalian DNA topoisomerase II. / Liu, Leroy-Fong; Rowe, T. C.; Yang, L.; Tewey, K. M.; Chen, G. L.

In: Journal of Biological Chemistry, Vol. 258, No. 24, 25.12.1983, p. 15365-15370.

Research output: Contribution to journalArticle

Liu, L-F, Rowe, TC, Yang, L, Tewey, KM & Chen, GL 1983, 'Cleavage of DNA by mammalian DNA topoisomerase II.', Journal of Biological Chemistry, vol. 258, no. 24, pp. 15365-15370.
Liu L-F, Rowe TC, Yang L, Tewey KM, Chen GL. Cleavage of DNA by mammalian DNA topoisomerase II. Journal of Biological Chemistry. 1983 Dec 25;258(24):15365-15370.
Liu, Leroy-Fong ; Rowe, T. C. ; Yang, L. ; Tewey, K. M. ; Chen, G. L. / Cleavage of DNA by mammalian DNA topoisomerase II. In: Journal of Biological Chemistry. 1983 ; Vol. 258, No. 24. pp. 15365-15370.
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