Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy

Mei Huei Lin, Young Chau Liu, Shyun Yeu Liu, Fang Chi Chen, Pei Jung Yang, Guan Hua Li, Pang Yen Liu, Ching Yu Yen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. Methods: We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells. Results: Both benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells. Conclusions: These results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program.

Original languageEnglish
Pages (from-to)25-31
JournalJournal of Oral Pathology and Medicine
Volume47
Issue number1
DOIs
Publication statusPublished - Jan 2018

Fingerprint

Clathrin
Autophagy
Endocytosis
Areca
Benzyl Alcohol
Dynamins
Nuts
Light
Small Interfering RNA
Microtubule-Associated Proteins
Down-Regulation
Carcinoma
Proteins
N'-(3,4-dihydroxybenzylidene)-3-hydroxy-2-naphthahydrazide

Keywords

  • Areca nut
  • Autophagy
  • Benzyl alcohol
  • Clathrin
  • Dynamin
  • Dynasore
  • Endocytosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

Cite this

Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy. / Lin, Mei Huei; Liu, Young Chau; Liu, Shyun Yeu; Chen, Fang Chi; Yang, Pei Jung; Li, Guan Hua; Liu, Pang Yen; Yen, Ching Yu.

In: Journal of Oral Pathology and Medicine, Vol. 47, No. 1, 01.2018, p. 25-31.

Research output: Contribution to journalArticle

Lin, Mei Huei ; Liu, Young Chau ; Liu, Shyun Yeu ; Chen, Fang Chi ; Yang, Pei Jung ; Li, Guan Hua ; Liu, Pang Yen ; Yen, Ching Yu. / Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy. In: Journal of Oral Pathology and Medicine. 2018 ; Vol. 47, No. 1. pp. 25-31.
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abstract = "Background: We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. Methods: We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells. Results: Both benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells. Conclusions: These results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program.",
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AU - Yang, Pei Jung

AU - Li, Guan Hua

AU - Liu, Pang Yen

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