Ciprofloxacin-resistant Salmonella enterica Typhimurium and Choleraesuis from Pigs to Humans, Taiwan

Po Ren Hsueh, Lee Jene Teng, Sung Pin Tseng, Chao Fu Chang, Jen Hsien Wan, Jing Jou Yan, Chun Ming Lee, Yin Ching Chuang, Wen Kuei Huang, Dine Yang, Jainn Ming Shyr, Kwok Woon Yu, Li Shin Wang, Jang Jih Lu, Wen Chien Ko, Jiunn Jong Wu, Feng Yee Chang, Yi Chueh Yang, Yeu Jun Lau, Yung Ching LiuCheng Yi Liu, Shen Wu Ho, Kwen Tay Luh

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Abstract

We evaluated the disk susceptibility data of 671 nontyphoid Salmonella isolates collected from different parts of Taiwan from March 2001 to August 2001 and 1,261 nontyphoid Salmonella isolates from the National Taiwan University Hospital from 1996 to 2001. Overall, ciprofloxacin resistance was found in 2.7% (18/671) of all nontyphoid Salmonella isolates, in 1.4% (5/347) of Salmonella enterica serotype Typhimurium and in 7.5% (8/107) in S. enterica serotype Choleraesuis nationwide. MICs of six newer fluoroquinolones were determined for the following isolates: 37 isolates of ciprofloxacin-resistant (human) S. Typhimurium (N = 26) and Choleraesuis (N = 11), 10 isolates of ciprofloxacin-susceptible (MIC 0.12 μg/mL). Sequence analysis of the gryA, gyrB, parC, parE, and acrR genes, ciprofloxacin accumulation, and genotypes generated by pulsed-field gel electrophoresis with three restriction enzymes (SpeI, XbaI, and BlnI) were performed. All 26 S. Typhimurium isolates from humans and pigs belonged to genotype I. For S. Choleraesuis isolates, 91% (10/11) of human isolates and 54% (7/13) of swine isolates belonged to genotype B. These two genotypes isolates from humans all exhibited a high-level of resistance to ciprofloxacin (MIC 16-64 mg/mL). They had two-base substitutions in the gyrA gene at codons 83 (Ser83Phe) and 87 (Asp87Gly or Asp87Asn) and in the parC gene at codon 80 (Ser80Arg, Ser80Ile, or Ser84Lys). Our investigation documented that not only did these two S. enterica isolates have a high prevalence of ciprofloxacin resistance nationwide but also that some closely related ciprofloxacin-resistant strains are disseminated from pigs to humans.

Original languageEnglish
Pages (from-to)60-68
Number of pages9
JournalEmerging Infectious Diseases
Volume10
Issue number1
Publication statusPublished - Jan 2004
Externally publishedYes

Fingerprint

Salmonella enterica
Salmonella typhimurium
Ciprofloxacin
Taiwan
Swine
Genotype
Salmonella
Codon
Genes
Pulsed Field Gel Electrophoresis
Fluoroquinolones
Sequence Analysis
Enzymes

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Hsueh, P. R., Teng, L. J., Tseng, S. P., Chang, C. F., Wan, J. H., Yan, J. J., ... Luh, K. T. (2004). Ciprofloxacin-resistant Salmonella enterica Typhimurium and Choleraesuis from Pigs to Humans, Taiwan. Emerging Infectious Diseases, 10(1), 60-68.

Ciprofloxacin-resistant Salmonella enterica Typhimurium and Choleraesuis from Pigs to Humans, Taiwan. / Hsueh, Po Ren; Teng, Lee Jene; Tseng, Sung Pin; Chang, Chao Fu; Wan, Jen Hsien; Yan, Jing Jou; Lee, Chun Ming; Chuang, Yin Ching; Huang, Wen Kuei; Yang, Dine; Shyr, Jainn Ming; Yu, Kwok Woon; Wang, Li Shin; Lu, Jang Jih; Ko, Wen Chien; Wu, Jiunn Jong; Chang, Feng Yee; Yang, Yi Chueh; Lau, Yeu Jun; Liu, Yung Ching; Liu, Cheng Yi; Ho, Shen Wu; Luh, Kwen Tay.

In: Emerging Infectious Diseases, Vol. 10, No. 1, 01.2004, p. 60-68.

Research output: Contribution to journalArticle

Hsueh, PR, Teng, LJ, Tseng, SP, Chang, CF, Wan, JH, Yan, JJ, Lee, CM, Chuang, YC, Huang, WK, Yang, D, Shyr, JM, Yu, KW, Wang, LS, Lu, JJ, Ko, WC, Wu, JJ, Chang, FY, Yang, YC, Lau, YJ, Liu, YC, Liu, CY, Ho, SW & Luh, KT 2004, 'Ciprofloxacin-resistant Salmonella enterica Typhimurium and Choleraesuis from Pigs to Humans, Taiwan', Emerging Infectious Diseases, vol. 10, no. 1, pp. 60-68.
Hsueh PR, Teng LJ, Tseng SP, Chang CF, Wan JH, Yan JJ et al. Ciprofloxacin-resistant Salmonella enterica Typhimurium and Choleraesuis from Pigs to Humans, Taiwan. Emerging Infectious Diseases. 2004 Jan;10(1):60-68.
Hsueh, Po Ren ; Teng, Lee Jene ; Tseng, Sung Pin ; Chang, Chao Fu ; Wan, Jen Hsien ; Yan, Jing Jou ; Lee, Chun Ming ; Chuang, Yin Ching ; Huang, Wen Kuei ; Yang, Dine ; Shyr, Jainn Ming ; Yu, Kwok Woon ; Wang, Li Shin ; Lu, Jang Jih ; Ko, Wen Chien ; Wu, Jiunn Jong ; Chang, Feng Yee ; Yang, Yi Chueh ; Lau, Yeu Jun ; Liu, Yung Ching ; Liu, Cheng Yi ; Ho, Shen Wu ; Luh, Kwen Tay. / Ciprofloxacin-resistant Salmonella enterica Typhimurium and Choleraesuis from Pigs to Humans, Taiwan. In: Emerging Infectious Diseases. 2004 ; Vol. 10, No. 1. pp. 60-68.
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abstract = "We evaluated the disk susceptibility data of 671 nontyphoid Salmonella isolates collected from different parts of Taiwan from March 2001 to August 2001 and 1,261 nontyphoid Salmonella isolates from the National Taiwan University Hospital from 1996 to 2001. Overall, ciprofloxacin resistance was found in 2.7{\%} (18/671) of all nontyphoid Salmonella isolates, in 1.4{\%} (5/347) of Salmonella enterica serotype Typhimurium and in 7.5{\%} (8/107) in S. enterica serotype Choleraesuis nationwide. MICs of six newer fluoroquinolones were determined for the following isolates: 37 isolates of ciprofloxacin-resistant (human) S. Typhimurium (N = 26) and Choleraesuis (N = 11), 10 isolates of ciprofloxacin-susceptible (MIC 0.12 μg/mL). Sequence analysis of the gryA, gyrB, parC, parE, and acrR genes, ciprofloxacin accumulation, and genotypes generated by pulsed-field gel electrophoresis with three restriction enzymes (SpeI, XbaI, and BlnI) were performed. All 26 S. Typhimurium isolates from humans and pigs belonged to genotype I. For S. Choleraesuis isolates, 91{\%} (10/11) of human isolates and 54{\%} (7/13) of swine isolates belonged to genotype B. These two genotypes isolates from humans all exhibited a high-level of resistance to ciprofloxacin (MIC 16-64 mg/mL). They had two-base substitutions in the gyrA gene at codons 83 (Ser83Phe) and 87 (Asp87Gly or Asp87Asn) and in the parC gene at codon 80 (Ser80Arg, Ser80Ile, or Ser84Lys). Our investigation documented that not only did these two S. enterica isolates have a high prevalence of ciprofloxacin resistance nationwide but also that some closely related ciprofloxacin-resistant strains are disseminated from pigs to humans.",
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T1 - Ciprofloxacin-resistant Salmonella enterica Typhimurium and Choleraesuis from Pigs to Humans, Taiwan

AU - Hsueh, Po Ren

AU - Teng, Lee Jene

AU - Tseng, Sung Pin

AU - Chang, Chao Fu

AU - Wan, Jen Hsien

AU - Yan, Jing Jou

AU - Lee, Chun Ming

AU - Chuang, Yin Ching

AU - Huang, Wen Kuei

AU - Yang, Dine

AU - Shyr, Jainn Ming

AU - Yu, Kwok Woon

AU - Wang, Li Shin

AU - Lu, Jang Jih

AU - Ko, Wen Chien

AU - Wu, Jiunn Jong

AU - Chang, Feng Yee

AU - Yang, Yi Chueh

AU - Lau, Yeu Jun

AU - Liu, Yung Ching

AU - Liu, Cheng Yi

AU - Ho, Shen Wu

AU - Luh, Kwen Tay

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N2 - We evaluated the disk susceptibility data of 671 nontyphoid Salmonella isolates collected from different parts of Taiwan from March 2001 to August 2001 and 1,261 nontyphoid Salmonella isolates from the National Taiwan University Hospital from 1996 to 2001. Overall, ciprofloxacin resistance was found in 2.7% (18/671) of all nontyphoid Salmonella isolates, in 1.4% (5/347) of Salmonella enterica serotype Typhimurium and in 7.5% (8/107) in S. enterica serotype Choleraesuis nationwide. MICs of six newer fluoroquinolones were determined for the following isolates: 37 isolates of ciprofloxacin-resistant (human) S. Typhimurium (N = 26) and Choleraesuis (N = 11), 10 isolates of ciprofloxacin-susceptible (MIC 0.12 μg/mL). Sequence analysis of the gryA, gyrB, parC, parE, and acrR genes, ciprofloxacin accumulation, and genotypes generated by pulsed-field gel electrophoresis with three restriction enzymes (SpeI, XbaI, and BlnI) were performed. All 26 S. Typhimurium isolates from humans and pigs belonged to genotype I. For S. Choleraesuis isolates, 91% (10/11) of human isolates and 54% (7/13) of swine isolates belonged to genotype B. These two genotypes isolates from humans all exhibited a high-level of resistance to ciprofloxacin (MIC 16-64 mg/mL). They had two-base substitutions in the gyrA gene at codons 83 (Ser83Phe) and 87 (Asp87Gly or Asp87Asn) and in the parC gene at codon 80 (Ser80Arg, Ser80Ile, or Ser84Lys). Our investigation documented that not only did these two S. enterica isolates have a high prevalence of ciprofloxacin resistance nationwide but also that some closely related ciprofloxacin-resistant strains are disseminated from pigs to humans.

AB - We evaluated the disk susceptibility data of 671 nontyphoid Salmonella isolates collected from different parts of Taiwan from March 2001 to August 2001 and 1,261 nontyphoid Salmonella isolates from the National Taiwan University Hospital from 1996 to 2001. Overall, ciprofloxacin resistance was found in 2.7% (18/671) of all nontyphoid Salmonella isolates, in 1.4% (5/347) of Salmonella enterica serotype Typhimurium and in 7.5% (8/107) in S. enterica serotype Choleraesuis nationwide. MICs of six newer fluoroquinolones were determined for the following isolates: 37 isolates of ciprofloxacin-resistant (human) S. Typhimurium (N = 26) and Choleraesuis (N = 11), 10 isolates of ciprofloxacin-susceptible (MIC 0.12 μg/mL). Sequence analysis of the gryA, gyrB, parC, parE, and acrR genes, ciprofloxacin accumulation, and genotypes generated by pulsed-field gel electrophoresis with three restriction enzymes (SpeI, XbaI, and BlnI) were performed. All 26 S. Typhimurium isolates from humans and pigs belonged to genotype I. For S. Choleraesuis isolates, 91% (10/11) of human isolates and 54% (7/13) of swine isolates belonged to genotype B. These two genotypes isolates from humans all exhibited a high-level of resistance to ciprofloxacin (MIC 16-64 mg/mL). They had two-base substitutions in the gyrA gene at codons 83 (Ser83Phe) and 87 (Asp87Gly or Asp87Asn) and in the parC gene at codon 80 (Ser80Arg, Ser80Ile, or Ser84Lys). Our investigation documented that not only did these two S. enterica isolates have a high prevalence of ciprofloxacin resistance nationwide but also that some closely related ciprofloxacin-resistant strains are disseminated from pigs to humans.

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