Cinnamophilin isolated from cinnamomum philippinense protects against collagen degradation in human chondrocytes

Yung Chang Lu, George Hsiao, Kuan Hung Lin, Ming-Shium Hsieh, Thanasekaran Jayakumar, Tian Shung Wu, Joen Rong Sheu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To investigate the therapeutic potential of naturally occurring cinnamophilin against cartilage degradation and its action mechanisms, its effects on matrix metalloproteinase (MMP)-1 and -13 induction were examined in the human SW1353 chondrocytic cell line. Human chondrocytes (SW1353) were stimulated with interleukin (IL)-1β, and then mitogen-activated protein kinase (MAPK) and c-Jun activations, inhibitory κB-α (IκB-α) degradation, and MMP-1, and 13 expressions were assayed by a Western blot analysis. Cinnamophilin strongly inhibited MMP-1 and -13 induction in IL-1β-treated (30 ng/mL) SW1353 cells in a concentration-dependent manner, and it also reduced MAPK family members including extracellular signal-regulated kinase (ERK), p38 MAPKs, and c-Jun N-terminal kinase. Moreover, nuclear factor (NF)-κB signaling activation through IκB-α degradation, IκB kinase (IKK)-α/β, and p-65 phosphorylation was restored by cinnamophilin upon IL-1β stimulation. Importantly, results showed that IL-1β-induced activation of phosphorylated (p)-c-Jun in chondrocytes was significantly inhibited by cinnamophilin. These results indicate that cinnamophilin inhibited MMP-1 and -13 expressions in IL-1β-treated chondrocytes through either NF-κB or ERK/p38 MAPK downregulation and/or suppressing p-c-Jun pathways. Furthermore, these findings suggest that cinnamophilin may have the potential for chondroprotection against collagen matrix breakdown in cartilage of diseased tissues such as those found in arthritic disorders.

Original languageEnglish
Pages (from-to)892-899
Number of pages8
JournalPhytotherapy Research
Volume27
Issue number6
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Cinnamomum
Chondrocytes
Matrix Metalloproteinase 13
Interleukin-1
Matrix Metalloproteinase 1
Collagen
Extracellular Signal-Regulated MAP Kinases
p38 Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
Cartilage Diseases
JNK Mitogen-Activated Protein Kinases
Arthritis
Cartilage
cinnamophilin
Phosphotransferases
Down-Regulation
Western Blotting
Phosphorylation
Cell Line

Keywords

  • c-Jun
  • cinnamophilin
  • human chondrosarcoma cells
  • IκB-α
  • IKK-α/β
  • IL-1β
  • MAPKs
  • MMPs

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cinnamophilin isolated from cinnamomum philippinense protects against collagen degradation in human chondrocytes. / Lu, Yung Chang; Hsiao, George; Lin, Kuan Hung; Hsieh, Ming-Shium; Jayakumar, Thanasekaran; Wu, Tian Shung; Sheu, Joen Rong.

In: Phytotherapy Research, Vol. 27, No. 6, 06.2013, p. 892-899.

Research output: Contribution to journalArticle

Lu, Yung Chang ; Hsiao, George ; Lin, Kuan Hung ; Hsieh, Ming-Shium ; Jayakumar, Thanasekaran ; Wu, Tian Shung ; Sheu, Joen Rong. / Cinnamophilin isolated from cinnamomum philippinense protects against collagen degradation in human chondrocytes. In: Phytotherapy Research. 2013 ; Vol. 27, No. 6. pp. 892-899.
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AU - Jayakumar, Thanasekaran

AU - Wu, Tian Shung

AU - Sheu, Joen Rong

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