Cinnamophilin as a novel antiperoxidative cytoprotectant and free radical scavenger

George Hsiao, Che Ming Teng, Joen Rong Sheu, Yu Wen Cheng, Kwok Keung Lam, Yen Mei Lee, Tian Shung Wu, Mao Hsiung Yen

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

The antioxidant properties of cinnamophilin were evaluated by studying its ability to react with relevant reactive oxygen species, and its protective effect on cultured cells and biomacromolecules under oxidative stress. Cinnamophilin concentration-dependently suppressed non-enzymatic iron-induced lipid peroxidation in rat brain homogenates with an IC50 value of 8.0±0.7 μM and iron ion/ADP/ascorbate-initiated rat liver mitochondrial lipid peroxidation with an IC50 value of 17.7±0.2 μM. It also exerted an inhibitory activity on NADPH-dependent microsomal lipid peroxidation with an IC50 value of 3.4±0.1 μM without affecting microsomal electron transport of NADPH-cytochrome P-450 reductase. Both 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azo-bis(2-amidinopropane) dihydrochloride-derived peroxyl radical tests demonstrated that cinnamophilin possessed marked free radical scavenging capacity. Cinnamophilin significantly protected cultured rat aortic smooth muscle cells (A7r5) against alloxan/iron ion/H2O2-induced damage resulting in cytoplasmic membranous disturbance and mitochondrial potential decay. By the way, cinnamophilin inhibited copper-catalyzed oxidation of human low-density lipoprotein, as measured by fluorescence intensity and thiobarbituric acid-reactive substance formation in a concentration-dependent manner. On the other hand, it was reactive toward superoxide anions generated by the xanthine/xanthine oxidase system and the aortic segment from aged spontaneously hypertensive rat. Furthermore, cinnamophilin exerted a divergent effect on the respiratory burst of human neutrophil by different stimulators. Our results show that cinnamophilin acts as a novel antioxidant and cytoprotectant against oxidative damage.

Original languageEnglish
Pages (from-to)77-88
Number of pages12
JournalBiochimica et Biophysica Acta - General Subjects
Volume1525
Issue number1-2
DOIs
Publication statusPublished - Feb 16 2001

Keywords

  • Antioxidant
  • Cinnamophilin
  • Cytoprotectant
  • Lipid peroxidation
  • Peroxyl radical
  • Superoxide anion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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