Chrysin suppresses IL-6-induced angiogenesis via down-regulation of JAK1/STAT3 and VEGF: An in vitro and inzovo approach

Chiu Mei Lin, Kou-Gi Shyu, Bao Wei Wang, Hang Chang, Yen Hsu Chen, Jen Hwey Chiu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane assay, an in ovo angiogenesis assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiangiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological angiogenesis.

Original languageEnglish
Pages (from-to)7082-7087
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number11
DOIs
Publication statusPublished - Jun 9 2010

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Keywords

  • Angiogenesis
  • Chrysin
  • Gp130/JAK1/STAT3
  • IL-6
  • VEGF

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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