Abstract

Objective. Few studies have investigated the feasibility of using chronic pudendal neuromodulation for improving voiding function in patients with diabetes who are also experiencing urinary retention. The present study investigated the effects of chronic electrical stimulation (ES) of the sensory branch of the pudendal nerve on voiding function in diabetic rats. Approach. A custom-made implantable microstimulation system was designed and manufactured for chronic implantation in normal control (NC) and diabetic rats. After three or six weeks of pudendal neuromodulation, the intravesical pressure, external urethral sphincter electromyograms (EUS-EMGs), and urine flow rate (UFR) of all rats were simultaneously recorded to assess the effects of chronic pudendal ES on voiding function. Morphological changes in pudendal axons were assessed through hematoxylin and eosin (H&E) staining. Results. Abnormal cystometric measurements, including an increased volume threshold, contraction amplitude, and residual volume (RV) as well as a decreased burst period (BP) indicated voiding dysfunction in the diabetic rats with sham ES for three or six weeks. However, real ES for three or six weeks reversed these abnormal results; these parameters became similar to those of the NC rats and, consequently, voiding efficiency (VE) increased significantly from 17%-45% to 51%-57%. The cross-sectional area and axonal density of the pudendal nerve decreased in all the diabetic rats with sham ES for six weeks; however, these values increased significantly after real ES for six weeks. Significance. This study demonstrated the feasibility of using chronic pudendal neuromodulation for improving voiding function in diabetic rats. These results may facilitate the development of an advanced neural prosthesis for restoring bladder function in clinical settings.

Original languageEnglish
Article number046001
JournalJournal of Neural Engineering
Volume13
Issue number4
DOIs
Publication statusPublished - May 17 2016

Fingerprint

Rats
Electric Stimulation
Pudendal Nerve
Neural prostheses
Rat control
Neural Prostheses
Medical problems
Residual Volume
Urinary Retention
Feasibility Studies
Electromyography
Urethra
Hematoxylin
Eosine Yellowish-(YS)
Axons
Flow rate
Urinary Bladder
Urine
Staining and Labeling
Pressure

Keywords

  • electrical stimulation
  • intravesical pressure
  • neuromodulation
  • voiding

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cellular and Molecular Neuroscience

Cite this

Chronic pudendal neuromodulation using an implantable microstimulator improves voiding function in diabetic rats. / Hsieh, Tsung Hsun; Lin, Yin Tsong; Chen, Shih Ching; Peng, Chih Wei.

In: Journal of Neural Engineering, Vol. 13, No. 4, 046001, 17.05.2016.

Research output: Contribution to journalArticle

@article{f2b1ed6662cb444ba9aa72c1528a5459,
title = "Chronic pudendal neuromodulation using an implantable microstimulator improves voiding function in diabetic rats",
abstract = "Objective. Few studies have investigated the feasibility of using chronic pudendal neuromodulation for improving voiding function in patients with diabetes who are also experiencing urinary retention. The present study investigated the effects of chronic electrical stimulation (ES) of the sensory branch of the pudendal nerve on voiding function in diabetic rats. Approach. A custom-made implantable microstimulation system was designed and manufactured for chronic implantation in normal control (NC) and diabetic rats. After three or six weeks of pudendal neuromodulation, the intravesical pressure, external urethral sphincter electromyograms (EUS-EMGs), and urine flow rate (UFR) of all rats were simultaneously recorded to assess the effects of chronic pudendal ES on voiding function. Morphological changes in pudendal axons were assessed through hematoxylin and eosin (H&E) staining. Results. Abnormal cystometric measurements, including an increased volume threshold, contraction amplitude, and residual volume (RV) as well as a decreased burst period (BP) indicated voiding dysfunction in the diabetic rats with sham ES for three or six weeks. However, real ES for three or six weeks reversed these abnormal results; these parameters became similar to those of the NC rats and, consequently, voiding efficiency (VE) increased significantly from 17{\%}-45{\%} to 51{\%}-57{\%}. The cross-sectional area and axonal density of the pudendal nerve decreased in all the diabetic rats with sham ES for six weeks; however, these values increased significantly after real ES for six weeks. Significance. This study demonstrated the feasibility of using chronic pudendal neuromodulation for improving voiding function in diabetic rats. These results may facilitate the development of an advanced neural prosthesis for restoring bladder function in clinical settings.",
keywords = "electrical stimulation, intravesical pressure, neuromodulation, voiding",
author = "Hsieh, {Tsung Hsun} and Lin, {Yin Tsong} and Chen, {Shih Ching} and Peng, {Chih Wei}",
year = "2016",
month = "5",
day = "17",
doi = "10.1088/1741-2560/13/4/046001",
language = "English",
volume = "13",
journal = "Journal of Neural Engineering",
issn = "1741-2560",
publisher = "IOP Publishing Ltd.",
number = "4",

}

TY - JOUR

T1 - Chronic pudendal neuromodulation using an implantable microstimulator improves voiding function in diabetic rats

AU - Hsieh, Tsung Hsun

AU - Lin, Yin Tsong

AU - Chen, Shih Ching

AU - Peng, Chih Wei

PY - 2016/5/17

Y1 - 2016/5/17

N2 - Objective. Few studies have investigated the feasibility of using chronic pudendal neuromodulation for improving voiding function in patients with diabetes who are also experiencing urinary retention. The present study investigated the effects of chronic electrical stimulation (ES) of the sensory branch of the pudendal nerve on voiding function in diabetic rats. Approach. A custom-made implantable microstimulation system was designed and manufactured for chronic implantation in normal control (NC) and diabetic rats. After three or six weeks of pudendal neuromodulation, the intravesical pressure, external urethral sphincter electromyograms (EUS-EMGs), and urine flow rate (UFR) of all rats were simultaneously recorded to assess the effects of chronic pudendal ES on voiding function. Morphological changes in pudendal axons were assessed through hematoxylin and eosin (H&E) staining. Results. Abnormal cystometric measurements, including an increased volume threshold, contraction amplitude, and residual volume (RV) as well as a decreased burst period (BP) indicated voiding dysfunction in the diabetic rats with sham ES for three or six weeks. However, real ES for three or six weeks reversed these abnormal results; these parameters became similar to those of the NC rats and, consequently, voiding efficiency (VE) increased significantly from 17%-45% to 51%-57%. The cross-sectional area and axonal density of the pudendal nerve decreased in all the diabetic rats with sham ES for six weeks; however, these values increased significantly after real ES for six weeks. Significance. This study demonstrated the feasibility of using chronic pudendal neuromodulation for improving voiding function in diabetic rats. These results may facilitate the development of an advanced neural prosthesis for restoring bladder function in clinical settings.

AB - Objective. Few studies have investigated the feasibility of using chronic pudendal neuromodulation for improving voiding function in patients with diabetes who are also experiencing urinary retention. The present study investigated the effects of chronic electrical stimulation (ES) of the sensory branch of the pudendal nerve on voiding function in diabetic rats. Approach. A custom-made implantable microstimulation system was designed and manufactured for chronic implantation in normal control (NC) and diabetic rats. After three or six weeks of pudendal neuromodulation, the intravesical pressure, external urethral sphincter electromyograms (EUS-EMGs), and urine flow rate (UFR) of all rats were simultaneously recorded to assess the effects of chronic pudendal ES on voiding function. Morphological changes in pudendal axons were assessed through hematoxylin and eosin (H&E) staining. Results. Abnormal cystometric measurements, including an increased volume threshold, contraction amplitude, and residual volume (RV) as well as a decreased burst period (BP) indicated voiding dysfunction in the diabetic rats with sham ES for three or six weeks. However, real ES for three or six weeks reversed these abnormal results; these parameters became similar to those of the NC rats and, consequently, voiding efficiency (VE) increased significantly from 17%-45% to 51%-57%. The cross-sectional area and axonal density of the pudendal nerve decreased in all the diabetic rats with sham ES for six weeks; however, these values increased significantly after real ES for six weeks. Significance. This study demonstrated the feasibility of using chronic pudendal neuromodulation for improving voiding function in diabetic rats. These results may facilitate the development of an advanced neural prosthesis for restoring bladder function in clinical settings.

KW - electrical stimulation

KW - intravesical pressure

KW - neuromodulation

KW - voiding

UR - http://www.scopus.com/inward/record.url?scp=84979581639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979581639&partnerID=8YFLogxK

U2 - 10.1088/1741-2560/13/4/046001

DO - 10.1088/1741-2560/13/4/046001

M3 - Article

C2 - 27187108

AN - SCOPUS:84979581639

VL - 13

JO - Journal of Neural Engineering

JF - Journal of Neural Engineering

SN - 1741-2560

IS - 4

M1 - 046001

ER -