Chromosome 19 open reading frame 80 is upregulated by thyroid hormone and modulates autophagy and lipid metabolism

Yi Hsin Tseng, Po Yuan Ke, Chia Jung Liao, Sheng Ming Wu, Hsiang Cheng Chi, Chung Ying Tsai, Cheng Yi Chen, Yang Hsiang Lin, Kwang Huei Lin

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The thyroid hormone, T3, regulates cell growth, differentiation and development through binding to the nuclear thyroid hormone receptor (THR), a member of the steroid/TR superfamily of ligand-dependent transcriptional factors. T3 modulates lipid metabolism in liver, although the detailed molecular mechanisms are unclear at present. Here, by a microarray analysis, we identified a novel chromosome 19 open reading frame 80 (C19orf80) which was activated by T3. T3 stimulation led to upregulation of both mRNA and protein levels of C19orf80. Immunofluorescence analysis revealed a vesicle-like pattern of C19orf80 around lipid droplets or within the lysosome-associated compartment in cells. Furthermore, T3 treatment as well as C19orf80 overexpression specifically activated the autophagic response and lipid metabolism, as observed from lipidated LC3 (LC3-II) and levels of oxygen consumption rate, respectively. Reciprocally, knockdown of C19orf80 obstructed T3-activated autophagy and lipolysis. Moreover, treatment with autolysosome maturation inhibitors, ammonium chloride and chloroquine, not only suppressed the T3-activated autophagic process but also lipid metabolism. Our results collectively suggested that T3 regulates lipid metabolism through a C19orf80- activated autophagic process.

Original languageEnglish
Pages (from-to)20-31
Number of pages12
JournalAutophagy
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 2014
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 19
Autophagy
Thyroid Hormones
Lipid Metabolism
Open Reading Frames
Thyroid Hormone Receptors
Ammonium Chloride
Lipolysis
Chloroquine
Triiodothyronine
Microarray Analysis
Cytoplasmic and Nuclear Receptors
Lysosomes
Growth and Development
Oxygen Consumption
Fluorescent Antibody Technique
Cell Differentiation
Up-Regulation
Steroids
Ligands

Keywords

  • Autophagy
  • Chromosome 19 open reading frame 80
  • Lipid droplet
  • Lysosome
  • Thyroid hormone

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Chromosome 19 open reading frame 80 is upregulated by thyroid hormone and modulates autophagy and lipid metabolism. / Tseng, Yi Hsin; Ke, Po Yuan; Liao, Chia Jung; Wu, Sheng Ming; Chi, Hsiang Cheng; Tsai, Chung Ying; Chen, Cheng Yi; Lin, Yang Hsiang; Lin, Kwang Huei.

In: Autophagy, Vol. 10, No. 1, 01.2014, p. 20-31.

Research output: Contribution to journalArticle

Tseng, YH, Ke, PY, Liao, CJ, Wu, SM, Chi, HC, Tsai, CY, Chen, CY, Lin, YH & Lin, KH 2014, 'Chromosome 19 open reading frame 80 is upregulated by thyroid hormone and modulates autophagy and lipid metabolism', Autophagy, vol. 10, no. 1, pp. 20-31. https://doi.org/10.4161/auto.26126
Tseng, Yi Hsin ; Ke, Po Yuan ; Liao, Chia Jung ; Wu, Sheng Ming ; Chi, Hsiang Cheng ; Tsai, Chung Ying ; Chen, Cheng Yi ; Lin, Yang Hsiang ; Lin, Kwang Huei. / Chromosome 19 open reading frame 80 is upregulated by thyroid hormone and modulates autophagy and lipid metabolism. In: Autophagy. 2014 ; Vol. 10, No. 1. pp. 20-31.
@article{b1c0adc2ad1b4311ae230e5aebcf4d01,
title = "Chromosome 19 open reading frame 80 is upregulated by thyroid hormone and modulates autophagy and lipid metabolism",
abstract = "The thyroid hormone, T3, regulates cell growth, differentiation and development through binding to the nuclear thyroid hormone receptor (THR), a member of the steroid/TR superfamily of ligand-dependent transcriptional factors. T3 modulates lipid metabolism in liver, although the detailed molecular mechanisms are unclear at present. Here, by a microarray analysis, we identified a novel chromosome 19 open reading frame 80 (C19orf80) which was activated by T3. T3 stimulation led to upregulation of both mRNA and protein levels of C19orf80. Immunofluorescence analysis revealed a vesicle-like pattern of C19orf80 around lipid droplets or within the lysosome-associated compartment in cells. Furthermore, T3 treatment as well as C19orf80 overexpression specifically activated the autophagic response and lipid metabolism, as observed from lipidated LC3 (LC3-II) and levels of oxygen consumption rate, respectively. Reciprocally, knockdown of C19orf80 obstructed T3-activated autophagy and lipolysis. Moreover, treatment with autolysosome maturation inhibitors, ammonium chloride and chloroquine, not only suppressed the T3-activated autophagic process but also lipid metabolism. Our results collectively suggested that T3 regulates lipid metabolism through a C19orf80- activated autophagic process.",
keywords = "Autophagy, Chromosome 19 open reading frame 80, Lipid droplet, Lysosome, Thyroid hormone",
author = "Tseng, {Yi Hsin} and Ke, {Po Yuan} and Liao, {Chia Jung} and Wu, {Sheng Ming} and Chi, {Hsiang Cheng} and Tsai, {Chung Ying} and Chen, {Cheng Yi} and Lin, {Yang Hsiang} and Lin, {Kwang Huei}",
year = "2014",
month = "1",
doi = "10.4161/auto.26126",
language = "English",
volume = "10",
pages = "20--31",
journal = "Autophagy",
issn = "1554-8627",
publisher = "Landes Bioscience",
number = "1",

}

TY - JOUR

T1 - Chromosome 19 open reading frame 80 is upregulated by thyroid hormone and modulates autophagy and lipid metabolism

AU - Tseng, Yi Hsin

AU - Ke, Po Yuan

AU - Liao, Chia Jung

AU - Wu, Sheng Ming

AU - Chi, Hsiang Cheng

AU - Tsai, Chung Ying

AU - Chen, Cheng Yi

AU - Lin, Yang Hsiang

AU - Lin, Kwang Huei

PY - 2014/1

Y1 - 2014/1

N2 - The thyroid hormone, T3, regulates cell growth, differentiation and development through binding to the nuclear thyroid hormone receptor (THR), a member of the steroid/TR superfamily of ligand-dependent transcriptional factors. T3 modulates lipid metabolism in liver, although the detailed molecular mechanisms are unclear at present. Here, by a microarray analysis, we identified a novel chromosome 19 open reading frame 80 (C19orf80) which was activated by T3. T3 stimulation led to upregulation of both mRNA and protein levels of C19orf80. Immunofluorescence analysis revealed a vesicle-like pattern of C19orf80 around lipid droplets or within the lysosome-associated compartment in cells. Furthermore, T3 treatment as well as C19orf80 overexpression specifically activated the autophagic response and lipid metabolism, as observed from lipidated LC3 (LC3-II) and levels of oxygen consumption rate, respectively. Reciprocally, knockdown of C19orf80 obstructed T3-activated autophagy and lipolysis. Moreover, treatment with autolysosome maturation inhibitors, ammonium chloride and chloroquine, not only suppressed the T3-activated autophagic process but also lipid metabolism. Our results collectively suggested that T3 regulates lipid metabolism through a C19orf80- activated autophagic process.

AB - The thyroid hormone, T3, regulates cell growth, differentiation and development through binding to the nuclear thyroid hormone receptor (THR), a member of the steroid/TR superfamily of ligand-dependent transcriptional factors. T3 modulates lipid metabolism in liver, although the detailed molecular mechanisms are unclear at present. Here, by a microarray analysis, we identified a novel chromosome 19 open reading frame 80 (C19orf80) which was activated by T3. T3 stimulation led to upregulation of both mRNA and protein levels of C19orf80. Immunofluorescence analysis revealed a vesicle-like pattern of C19orf80 around lipid droplets or within the lysosome-associated compartment in cells. Furthermore, T3 treatment as well as C19orf80 overexpression specifically activated the autophagic response and lipid metabolism, as observed from lipidated LC3 (LC3-II) and levels of oxygen consumption rate, respectively. Reciprocally, knockdown of C19orf80 obstructed T3-activated autophagy and lipolysis. Moreover, treatment with autolysosome maturation inhibitors, ammonium chloride and chloroquine, not only suppressed the T3-activated autophagic process but also lipid metabolism. Our results collectively suggested that T3 regulates lipid metabolism through a C19orf80- activated autophagic process.

KW - Autophagy

KW - Chromosome 19 open reading frame 80

KW - Lipid droplet

KW - Lysosome

KW - Thyroid hormone

UR - http://www.scopus.com/inward/record.url?scp=84894442654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894442654&partnerID=8YFLogxK

U2 - 10.4161/auto.26126

DO - 10.4161/auto.26126

M3 - Article

VL - 10

SP - 20

EP - 31

JO - Autophagy

JF - Autophagy

SN - 1554-8627

IS - 1

ER -