Chromosomal control of wheat gliadin

analysis by reversed-phase high-performance liquid chromatography

J. A. Bietz, T. Burnouf

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Gliadin proteins of the hexaploid wheat variety 'Chinese Spring', and of its nullisomic-tetrasomic and ditelocentric aneuploid lines, were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). Reversed-phase separations were carried out at 70°C on C8 and C18 columns using a gradient of increasing acetonitrile concentration in the presence of 0.1% trifluoroacetic acid. Thirty-five components were separated and all were found to be controlled by genes on the short arms of group 1 and group 6 chromosomes (the complex Gli-1 and Gli-2 loci). Results indicated that gluten polypeptides elute as groups in order of increasing hydrophobicity in the following approximate order: (1) albumins plus globulins, (2) ω-gliadins, (3) high molecular weight (MW) glutenin subunits, (4) α-type gliadins, (5) low MW glutenin subunits, and (6) γ-gliadins. The three distinct protein types coded by genes at the complex Gli-I loci (ω-gliadins, γ-gliadins, and low MW glutenin subunits) thus have uniquely different surface hydrophobicities. Similarly, gene locations for hexaploid 'Cheyenne' gliadins and durum gliadin proteins in the varieties 'Langdon', 'Edmore', and 'Kharkovskaya-5' were determined through RP-HPLC analysis of aneuploid lines. All results confirm known locations of genes for gliadin proteins, and demonstrate that RP-HPLC is a powerful new tool for analysis of gliadins in breeding and genetic studies.

Original languageEnglish
Pages (from-to)599-609
Number of pages11
JournalTheoretical And Applied Genetics
Volume70
Issue number6
DOIs
Publication statusPublished - Sep 1985
Externally publishedYes

Fingerprint

Gliadin
gliadin
reversed-phase high performance liquid chromatography
Reverse-Phase Chromatography
Triticum
High Pressure Liquid Chromatography
wheat
glutenins
Molecular Weight
aneuploidy
Aneuploidy
molecular weight
hexaploidy
hydrophobicity
Hydrophobic and Hydrophilic Interactions
Proteins
genes
proteins
nullisomics
tetrasomics

Keywords

  • Chromosomes
  • Coding
  • Control
  • Gliadin
  • RP-HPLC
  • Triticum

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics
  • Plant Science
  • Horticulture
  • Agronomy and Crop Science

Cite this

Chromosomal control of wheat gliadin : analysis by reversed-phase high-performance liquid chromatography. / Bietz, J. A.; Burnouf, T.

In: Theoretical And Applied Genetics, Vol. 70, No. 6, 09.1985, p. 599-609.

Research output: Contribution to journalArticle

@article{9e411e96d9134652abebdbeda5371068,
title = "Chromosomal control of wheat gliadin: analysis by reversed-phase high-performance liquid chromatography",
abstract = "Gliadin proteins of the hexaploid wheat variety 'Chinese Spring', and of its nullisomic-tetrasomic and ditelocentric aneuploid lines, were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). Reversed-phase separations were carried out at 70°C on C8 and C18 columns using a gradient of increasing acetonitrile concentration in the presence of 0.1{\%} trifluoroacetic acid. Thirty-five components were separated and all were found to be controlled by genes on the short arms of group 1 and group 6 chromosomes (the complex Gli-1 and Gli-2 loci). Results indicated that gluten polypeptides elute as groups in order of increasing hydrophobicity in the following approximate order: (1) albumins plus globulins, (2) ω-gliadins, (3) high molecular weight (MW) glutenin subunits, (4) α-type gliadins, (5) low MW glutenin subunits, and (6) γ-gliadins. The three distinct protein types coded by genes at the complex Gli-I loci (ω-gliadins, γ-gliadins, and low MW glutenin subunits) thus have uniquely different surface hydrophobicities. Similarly, gene locations for hexaploid 'Cheyenne' gliadins and durum gliadin proteins in the varieties 'Langdon', 'Edmore', and 'Kharkovskaya-5' were determined through RP-HPLC analysis of aneuploid lines. All results confirm known locations of genes for gliadin proteins, and demonstrate that RP-HPLC is a powerful new tool for analysis of gliadins in breeding and genetic studies.",
keywords = "Chromosomes, Coding, Control, Gliadin, RP-HPLC, Triticum",
author = "Bietz, {J. A.} and T. Burnouf",
year = "1985",
month = "9",
doi = "10.1007/BF00252285",
language = "English",
volume = "70",
pages = "599--609",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Chromosomal control of wheat gliadin

T2 - analysis by reversed-phase high-performance liquid chromatography

AU - Bietz, J. A.

AU - Burnouf, T.

PY - 1985/9

Y1 - 1985/9

N2 - Gliadin proteins of the hexaploid wheat variety 'Chinese Spring', and of its nullisomic-tetrasomic and ditelocentric aneuploid lines, were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). Reversed-phase separations were carried out at 70°C on C8 and C18 columns using a gradient of increasing acetonitrile concentration in the presence of 0.1% trifluoroacetic acid. Thirty-five components were separated and all were found to be controlled by genes on the short arms of group 1 and group 6 chromosomes (the complex Gli-1 and Gli-2 loci). Results indicated that gluten polypeptides elute as groups in order of increasing hydrophobicity in the following approximate order: (1) albumins plus globulins, (2) ω-gliadins, (3) high molecular weight (MW) glutenin subunits, (4) α-type gliadins, (5) low MW glutenin subunits, and (6) γ-gliadins. The three distinct protein types coded by genes at the complex Gli-I loci (ω-gliadins, γ-gliadins, and low MW glutenin subunits) thus have uniquely different surface hydrophobicities. Similarly, gene locations for hexaploid 'Cheyenne' gliadins and durum gliadin proteins in the varieties 'Langdon', 'Edmore', and 'Kharkovskaya-5' were determined through RP-HPLC analysis of aneuploid lines. All results confirm known locations of genes for gliadin proteins, and demonstrate that RP-HPLC is a powerful new tool for analysis of gliadins in breeding and genetic studies.

AB - Gliadin proteins of the hexaploid wheat variety 'Chinese Spring', and of its nullisomic-tetrasomic and ditelocentric aneuploid lines, were analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC). Reversed-phase separations were carried out at 70°C on C8 and C18 columns using a gradient of increasing acetonitrile concentration in the presence of 0.1% trifluoroacetic acid. Thirty-five components were separated and all were found to be controlled by genes on the short arms of group 1 and group 6 chromosomes (the complex Gli-1 and Gli-2 loci). Results indicated that gluten polypeptides elute as groups in order of increasing hydrophobicity in the following approximate order: (1) albumins plus globulins, (2) ω-gliadins, (3) high molecular weight (MW) glutenin subunits, (4) α-type gliadins, (5) low MW glutenin subunits, and (6) γ-gliadins. The three distinct protein types coded by genes at the complex Gli-I loci (ω-gliadins, γ-gliadins, and low MW glutenin subunits) thus have uniquely different surface hydrophobicities. Similarly, gene locations for hexaploid 'Cheyenne' gliadins and durum gliadin proteins in the varieties 'Langdon', 'Edmore', and 'Kharkovskaya-5' were determined through RP-HPLC analysis of aneuploid lines. All results confirm known locations of genes for gliadin proteins, and demonstrate that RP-HPLC is a powerful new tool for analysis of gliadins in breeding and genetic studies.

KW - Chromosomes

KW - Coding

KW - Control

KW - Gliadin

KW - RP-HPLC

KW - Triticum

UR - http://www.scopus.com/inward/record.url?scp=0000042153&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000042153&partnerID=8YFLogxK

U2 - 10.1007/BF00252285

DO - 10.1007/BF00252285

M3 - Article

VL - 70

SP - 599

EP - 609

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 6

ER -