Chromatographic removal of viruses from plasma derivatives.

Research output: Contribution to journalReview article

27 Citations (Scopus)

Abstract

Progress in protein separation technology has led to the development of a new generation of plasma derivatives, generally prepared by procedures involving one or several chromatographic steps. In addition to providing two to three log purification factors of several therapeutic products, with regard to some protein contaminants, chromatography has been shown to improve the potential safety of new plasma derivatives by contributing to the removal of plasma-borne viruses. Indeed, validation studies have demonstrated that each immuno-affinity, ion-exchange, and heparin affinity chromatography step can eliminate 1 to 5 log of HIV-1, or of several model viruses, enveloped or non-enveloped, such as sindbis virus, pseudorabies virus, vesicular stomatitis virus, reovirus 3, or simian virus 40. Several parameters can be considered as influencing the chromatographic behaviour of viruses, including the size, shape, symmetry, and membrane structure. In addition, buffer conditions that may induce aggregation and change their apparent size and surface properties, as well as chromatography flow-rate and packing material characteristics, are, among others, important parameters potentially influencing the binding of viruses on chromatographic resins. Due to the complexity of the phenomena potentially influencing the chromatographic behaviour of viruses, and because these are not well understood, it is important to design chromatographic production processes of plasma derivatives and to perform their validation studies following a rigorous scientific approach.

Original languageEnglish
Pages (from-to)199-209
Number of pages11
JournalDevelopments in Biological Standardization
Volume81
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Viruses
Validation Studies
Chromatography
Mammalian orthoreovirus 3
Sindbis Virus
Suid Herpesvirus 1
Virus Attachment
Vesicular Stomatitis
Simian virus 40
Surface Properties
Ion Exchange
Affinity Chromatography
Heparin
HIV-1
Buffers
Proteins
Technology
Safety
Membranes
Therapeutics

ASJC Scopus subject areas

  • Biotechnology

Cite this

Chromatographic removal of viruses from plasma derivatives. / Burnouf, T.

In: Developments in Biological Standardization, Vol. 81, 1993, p. 199-209.

Research output: Contribution to journalReview article

@article{2f01cdc88fd146d49b2006dc73b9147f,
title = "Chromatographic removal of viruses from plasma derivatives.",
abstract = "Progress in protein separation technology has led to the development of a new generation of plasma derivatives, generally prepared by procedures involving one or several chromatographic steps. In addition to providing two to three log purification factors of several therapeutic products, with regard to some protein contaminants, chromatography has been shown to improve the potential safety of new plasma derivatives by contributing to the removal of plasma-borne viruses. Indeed, validation studies have demonstrated that each immuno-affinity, ion-exchange, and heparin affinity chromatography step can eliminate 1 to 5 log of HIV-1, or of several model viruses, enveloped or non-enveloped, such as sindbis virus, pseudorabies virus, vesicular stomatitis virus, reovirus 3, or simian virus 40. Several parameters can be considered as influencing the chromatographic behaviour of viruses, including the size, shape, symmetry, and membrane structure. In addition, buffer conditions that may induce aggregation and change their apparent size and surface properties, as well as chromatography flow-rate and packing material characteristics, are, among others, important parameters potentially influencing the binding of viruses on chromatographic resins. Due to the complexity of the phenomena potentially influencing the chromatographic behaviour of viruses, and because these are not well understood, it is important to design chromatographic production processes of plasma derivatives and to perform their validation studies following a rigorous scientific approach.",
author = "T. Burnouf",
year = "1993",
language = "English",
volume = "81",
pages = "199--209",
journal = "Developments in Biological Standardization",
issn = "0301-5149",
publisher = "S. Karger AG",

}

TY - JOUR

T1 - Chromatographic removal of viruses from plasma derivatives.

AU - Burnouf, T.

PY - 1993

Y1 - 1993

N2 - Progress in protein separation technology has led to the development of a new generation of plasma derivatives, generally prepared by procedures involving one or several chromatographic steps. In addition to providing two to three log purification factors of several therapeutic products, with regard to some protein contaminants, chromatography has been shown to improve the potential safety of new plasma derivatives by contributing to the removal of plasma-borne viruses. Indeed, validation studies have demonstrated that each immuno-affinity, ion-exchange, and heparin affinity chromatography step can eliminate 1 to 5 log of HIV-1, or of several model viruses, enveloped or non-enveloped, such as sindbis virus, pseudorabies virus, vesicular stomatitis virus, reovirus 3, or simian virus 40. Several parameters can be considered as influencing the chromatographic behaviour of viruses, including the size, shape, symmetry, and membrane structure. In addition, buffer conditions that may induce aggregation and change their apparent size and surface properties, as well as chromatography flow-rate and packing material characteristics, are, among others, important parameters potentially influencing the binding of viruses on chromatographic resins. Due to the complexity of the phenomena potentially influencing the chromatographic behaviour of viruses, and because these are not well understood, it is important to design chromatographic production processes of plasma derivatives and to perform their validation studies following a rigorous scientific approach.

AB - Progress in protein separation technology has led to the development of a new generation of plasma derivatives, generally prepared by procedures involving one or several chromatographic steps. In addition to providing two to three log purification factors of several therapeutic products, with regard to some protein contaminants, chromatography has been shown to improve the potential safety of new plasma derivatives by contributing to the removal of plasma-borne viruses. Indeed, validation studies have demonstrated that each immuno-affinity, ion-exchange, and heparin affinity chromatography step can eliminate 1 to 5 log of HIV-1, or of several model viruses, enveloped or non-enveloped, such as sindbis virus, pseudorabies virus, vesicular stomatitis virus, reovirus 3, or simian virus 40. Several parameters can be considered as influencing the chromatographic behaviour of viruses, including the size, shape, symmetry, and membrane structure. In addition, buffer conditions that may induce aggregation and change their apparent size and surface properties, as well as chromatography flow-rate and packing material characteristics, are, among others, important parameters potentially influencing the binding of viruses on chromatographic resins. Due to the complexity of the phenomena potentially influencing the chromatographic behaviour of viruses, and because these are not well understood, it is important to design chromatographic production processes of plasma derivatives and to perform their validation studies following a rigorous scientific approach.

UR - http://www.scopus.com/inward/record.url?scp=0027828722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027828722&partnerID=8YFLogxK

M3 - Review article

C2 - 8174804

AN - SCOPUS:0027828722

VL - 81

SP - 199

EP - 209

JO - Developments in Biological Standardization

JF - Developments in Biological Standardization

SN - 0301-5149

ER -