Chondroprotective role of sesamol by inhibiting MMPs expression via retaining NF-κB signaling in activated SW1353 cells

Yung Chang Lu, Thanasekaran Jayakumar, Yeh Fang Duann, Yung Chen Chou, Cheng Ying Hsieh, Shin Yun Yu, Joen Rong Sheu, George Hsiao

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Overexpression of matrix metalloproteinases (MMPs) is a major pathological factor causing cartilage destruction in osteoarthritis (OA). This study aimed to investigate the effects and mechanisms of sesamol on expression of MMPs in activated chondrosarcoma cells. Sesamol significantly attenuated TNF-α-and IL-1β-induced gelatinolysis and expression of MMP-9 in a concentration-dependent manner in SW1353 cells. Additionally, both MMP-1 and -13 stimulated by PMA were inhibited by sesamol. On the other hand, the NF-κB signaling activation through IκB-α degradation was restored by sesamol under TNF-R or PMA stimulation. Furthermore, this bioactive compound exerted the reduction on phosphorylation of ERK1/2 or p38 MAPKs after either PMA or IL-1β stimulation. This study also evaluated whether sesamol down-regulates MMP expression in the joint cartilage of monosodium iodoacetate (MIA)-induced OA in rats. Sesamol prevented the expression of MMP-1 and -9 in the cartilage of MIAinduced OA in rats. The results of this study demonstrate that sesamol inhibits cytokine- or PMA-induced MMPs expression through the signal pathways of either NF-κB or ERK/p38 MAPKs down-regulation. This study also showed that sesamol attenuates destructive factor expression in vivo, providing a potential strategy for the chondroprotective therapy in OA.

Original languageEnglish
Pages (from-to)4969-4978
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number9
DOIs
Publication statusPublished - May 11 2011

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Keywords

  • B-α
  • Human chondrosarcoma cells
  • IL-1β
  • MAPKs
  • MMPs
  • PMA
  • Sesamol
  • TNF-α

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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