Chondrogenesis from immortalized human mesenchymal stem cells: Comparison between collagen gel and pellet culture methods

Chih Hung Chang, Hsin Yi Lin, Hsu Wei Fang, Siow Tung Loo, Shih Chieh Hung, Yi Ching Ho, Chia Chun Chen, Feng Huei Lin, Hwa Chang Liu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Human mesenchymal stem cells (hMSCs) can differentiate into cells of connective tissue lineages, including cartilage. To overcome the limiting autogenous chondrocyte populations available in cartilage repair, various methods have been developed to maximize chondrogenesis of hMSCs in vitro, most of which use cells derived from primary culture. In this study, we compared chondrogenesis of immortalized hMSCs embedded in collagen gel to those grown in pellet culture. The hMSCs in collagen scaffolds expressed more glycosaminoglycan than those in pellet culture. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis demonstrated that the expression of genes encoding sox-9, aggrecan, and types I and II collagen increased in pellet culture over time. However, in the collagen cultures, only type II collagen and aggrecan expression increased over time, whereas sox-9 expression remained unchanged and type I collagen expression decreased. These results indicate that the immortalized hMSC line is a promising tool for further in vitro chondrogenic studies.

Original languageEnglish
Pages (from-to)561-566
Number of pages6
JournalArtificial Organs
Volume32
Issue number7
DOIs
Publication statusPublished - Jul 1 2008
Externally publishedYes

Fingerprint

Chondrogenesis
Stem cells
Mesenchymal Stromal Cells
Cell culture
Collagen
Gels
Aggrecans
Collagen Type II
Cartilage
Collagen Type I
Connective Tissue Cells
Gene encoding
Primary Cell Culture
Polymerase chain reaction
RNA-Directed DNA Polymerase
Scaffolds (biology)
Chondrocytes
Glycosaminoglycans
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction

Keywords

  • Biodegradable materials
  • Cartilage tissue repair
  • Chondrogenesis
  • Collagen scaffold
  • Human mesenchymal stem cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Chondrogenesis from immortalized human mesenchymal stem cells : Comparison between collagen gel and pellet culture methods. / Chang, Chih Hung; Lin, Hsin Yi; Fang, Hsu Wei; Loo, Siow Tung; Hung, Shih Chieh; Ho, Yi Ching; Chen, Chia Chun; Lin, Feng Huei; Liu, Hwa Chang.

In: Artificial Organs, Vol. 32, No. 7, 01.07.2008, p. 561-566.

Research output: Contribution to journalArticle

Chang, Chih Hung ; Lin, Hsin Yi ; Fang, Hsu Wei ; Loo, Siow Tung ; Hung, Shih Chieh ; Ho, Yi Ching ; Chen, Chia Chun ; Lin, Feng Huei ; Liu, Hwa Chang. / Chondrogenesis from immortalized human mesenchymal stem cells : Comparison between collagen gel and pellet culture methods. In: Artificial Organs. 2008 ; Vol. 32, No. 7. pp. 561-566.
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