Chondrocyte acting as phagocyte to internalize polyethylene wear particles and leads to the elevations of osteoarthritis associated NO and PGE2

Chih Hung Chang, Hsu Wei Fang, Yi Ching Ho, Huei Ting Huang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

It remains a mystery about the role of chondrocyte or cartilage on the co-existence of ultra-high molecular weight polyethylene (UHMWPE) wear particles from partial joint arthroplasty. An inverted co-culture system was performed to investigate the interactions between chondrocytes and UHMWPE wear particles. It was first time observed that chondrocytes can engulf UHMWPE particles and release osteoarthritis associated pro-inflammatory factors. TEM observation and flow cytometric analysis demonstrated the phagocytosis of particles by chondrocytes. It was found that polyethylene particles may reduce the viability of chondrocytes, and enhance the secretion of nitric oxide (NO) and PGE2. In conclusion, all these phenomena may contribute to further cartilage degeneration after partial joint arthroplasty surgery. It is first identified in this study that the chondrocyte acts as phagocyte to internalize wear particles and leads to the elevations of precursor mediators of osteoarthritis.

Original languageEnglish
Pages (from-to)884-888
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume369
Issue number3
DOIs
Publication statusPublished - May 9 2008
Externally publishedYes

Fingerprint

Polyethylene
Phagocytes
Chondrocytes
Dinoprostone
Osteoarthritis
Arthroplasty
Nitric Oxide
Wear of materials
Cartilage
Surgery
Joints
Transmission electron microscopy
Coculture Techniques
Phagocytosis
ultra-high molecular weight polyethylene
Observation

Keywords

  • Chondrocyte
  • Partial joint arthroplasty
  • Phagocytosis
  • UHMWPE
  • Wear particles

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chondrocyte acting as phagocyte to internalize polyethylene wear particles and leads to the elevations of osteoarthritis associated NO and PGE2. / Chang, Chih Hung; Fang, Hsu Wei; Ho, Yi Ching; Huang, Huei Ting.

In: Biochemical and Biophysical Research Communications, Vol. 369, No. 3, 09.05.2008, p. 884-888.

Research output: Contribution to journalArticle

@article{b0d280ae434f43aa807ae0fe265c4801,
title = "Chondrocyte acting as phagocyte to internalize polyethylene wear particles and leads to the elevations of osteoarthritis associated NO and PGE2",
abstract = "It remains a mystery about the role of chondrocyte or cartilage on the co-existence of ultra-high molecular weight polyethylene (UHMWPE) wear particles from partial joint arthroplasty. An inverted co-culture system was performed to investigate the interactions between chondrocytes and UHMWPE wear particles. It was first time observed that chondrocytes can engulf UHMWPE particles and release osteoarthritis associated pro-inflammatory factors. TEM observation and flow cytometric analysis demonstrated the phagocytosis of particles by chondrocytes. It was found that polyethylene particles may reduce the viability of chondrocytes, and enhance the secretion of nitric oxide (NO) and PGE2. In conclusion, all these phenomena may contribute to further cartilage degeneration after partial joint arthroplasty surgery. It is first identified in this study that the chondrocyte acts as phagocyte to internalize wear particles and leads to the elevations of precursor mediators of osteoarthritis.",
keywords = "Chondrocyte, Partial joint arthroplasty, Phagocytosis, UHMWPE, Wear particles",
author = "Chang, {Chih Hung} and Fang, {Hsu Wei} and Ho, {Yi Ching} and Huang, {Huei Ting}",
year = "2008",
month = "5",
day = "9",
doi = "10.1016/j.bbrc.2008.02.123",
language = "English",
volume = "369",
pages = "884--888",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "3",

}

TY - JOUR

T1 - Chondrocyte acting as phagocyte to internalize polyethylene wear particles and leads to the elevations of osteoarthritis associated NO and PGE2

AU - Chang, Chih Hung

AU - Fang, Hsu Wei

AU - Ho, Yi Ching

AU - Huang, Huei Ting

PY - 2008/5/9

Y1 - 2008/5/9

N2 - It remains a mystery about the role of chondrocyte or cartilage on the co-existence of ultra-high molecular weight polyethylene (UHMWPE) wear particles from partial joint arthroplasty. An inverted co-culture system was performed to investigate the interactions between chondrocytes and UHMWPE wear particles. It was first time observed that chondrocytes can engulf UHMWPE particles and release osteoarthritis associated pro-inflammatory factors. TEM observation and flow cytometric analysis demonstrated the phagocytosis of particles by chondrocytes. It was found that polyethylene particles may reduce the viability of chondrocytes, and enhance the secretion of nitric oxide (NO) and PGE2. In conclusion, all these phenomena may contribute to further cartilage degeneration after partial joint arthroplasty surgery. It is first identified in this study that the chondrocyte acts as phagocyte to internalize wear particles and leads to the elevations of precursor mediators of osteoarthritis.

AB - It remains a mystery about the role of chondrocyte or cartilage on the co-existence of ultra-high molecular weight polyethylene (UHMWPE) wear particles from partial joint arthroplasty. An inverted co-culture system was performed to investigate the interactions between chondrocytes and UHMWPE wear particles. It was first time observed that chondrocytes can engulf UHMWPE particles and release osteoarthritis associated pro-inflammatory factors. TEM observation and flow cytometric analysis demonstrated the phagocytosis of particles by chondrocytes. It was found that polyethylene particles may reduce the viability of chondrocytes, and enhance the secretion of nitric oxide (NO) and PGE2. In conclusion, all these phenomena may contribute to further cartilage degeneration after partial joint arthroplasty surgery. It is first identified in this study that the chondrocyte acts as phagocyte to internalize wear particles and leads to the elevations of precursor mediators of osteoarthritis.

KW - Chondrocyte

KW - Partial joint arthroplasty

KW - Phagocytosis

KW - UHMWPE

KW - Wear particles

UR - http://www.scopus.com/inward/record.url?scp=41149115986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41149115986&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2008.02.123

DO - 10.1016/j.bbrc.2008.02.123

M3 - Article

C2 - 18328808

AN - SCOPUS:41149115986

VL - 369

SP - 884

EP - 888

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -