Chondrocyte acting as phagocyte to internalize polyethylene wear particles and leads to the elevations of osteoarthritis associated NO and PGE2

Chih Hung Chang, Hsu Wei Fang, Yi Ching Ho, Huei Ting Huang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

It remains a mystery about the role of chondrocyte or cartilage on the co-existence of ultra-high molecular weight polyethylene (UHMWPE) wear particles from partial joint arthroplasty. An inverted co-culture system was performed to investigate the interactions between chondrocytes and UHMWPE wear particles. It was first time observed that chondrocytes can engulf UHMWPE particles and release osteoarthritis associated pro-inflammatory factors. TEM observation and flow cytometric analysis demonstrated the phagocytosis of particles by chondrocytes. It was found that polyethylene particles may reduce the viability of chondrocytes, and enhance the secretion of nitric oxide (NO) and PGE2. In conclusion, all these phenomena may contribute to further cartilage degeneration after partial joint arthroplasty surgery. It is first identified in this study that the chondrocyte acts as phagocyte to internalize wear particles and leads to the elevations of precursor mediators of osteoarthritis.

Original languageEnglish
Pages (from-to)884-888
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume369
Issue number3
DOIs
Publication statusPublished - May 9 2008
Externally publishedYes

Keywords

  • Chondrocyte
  • Partial joint arthroplasty
  • Phagocytosis
  • UHMWPE
  • Wear particles

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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