Cholinergic synaptic potentials in the supragranular layers of auditory cortex

June Horng Lue, Seu H. Chen, Jeng Yung Shieh, Chen Yuan Wen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Receptive-field plasticity within the auditory neocortex is associated with learning, memory, and acetylcholine (ACh). However, the interplay of elements involved in changing receptive-fields remains unclear. Herein, we describe a depolarizing and a hyperpolarizing potential elicited by repetitive stimulation (20-100 Hz, 0.5-2 sec) and dependent on ACh, which may be involved in modifying receptive-fields. These potentials were recorded, using whole cell techniques, in layer II/III pyramidal cells in the rat auditory cortex in vitro. Stimulation at low stimulus intensities can give rise to a hyperpolarizing response and stimulation at higher stimulus intensities can elicit a depolarizing response. The depolarizing response had a reversal potential of -35 mV, and was reduced by the combination of AMPA/kainate and NMDA glutamate receptor antagonists (AMPA/kainate: CNQX, DNQX, and GYKI 52466; NMDA: APV, MK-801) and by the muscarinic ACh receptor antagonist atropine. The hyperpolarizing response had a reversal potential of -73 mV and could be reduced by atropine, GABAA receptor antagonists (bicuculline and a Cl- channel blocker picrotoxin), and to a small extent a GABAB receptor antagonist (saclofen). This suggests that the hyperpolarizing response is likely to be mediated by ACh acting on GABAergic interneurons. Extracellular recordings, also made from layer II/III of cortical slices, yielded a negative-going potential which was reduced by ionotropic glutamate receptor antagonists (same as above) and by the ACh receptor antagonists atropine and scopolamine, suggesting that this potential was the extracellular representation of the depolarizing response.

Original languageEnglish
Pages (from-to)118-130
Number of pages13
JournalSynapse
Volume41
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Synaptic Potentials
Auditory Cortex
Atropine
Cholinergic Agents
Acetylcholine
Excitatory Amino Acid Antagonists
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Kainic Acid
Cholinergic Antagonists
6-Cyano-7-nitroquinoxaline-2,3-dione
Ionotropic Glutamate Receptors
GABA-A Receptor Antagonists
Picrotoxin
Scopolamine Hydrobromide
Dizocilpine Maleate
Bicuculline
Pyramidal Cells
Neocortex
Cholinergic Receptors
Muscarinic Receptors

Keywords

  • ACh
  • Chloride
  • GABA-A
  • GABA-B
  • Glutamate
  • Synaptic

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)
  • Pharmacology

Cite this

Cholinergic synaptic potentials in the supragranular layers of auditory cortex. / Lue, June Horng; Chen, Seu H.; Shieh, Jeng Yung; Wen, Chen Yuan.

In: Synapse, Vol. 41, No. 2, 2001, p. 118-130.

Research output: Contribution to journalArticle

Lue, June Horng ; Chen, Seu H. ; Shieh, Jeng Yung ; Wen, Chen Yuan. / Cholinergic synaptic potentials in the supragranular layers of auditory cortex. In: Synapse. 2001 ; Vol. 41, No. 2. pp. 118-130.
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