Cholesterol-3-beta, 5-alpha, 6-beta-triol induced genotoxicity through reactive oxygen species formation

Y. W. Cheng, J. J. Kang, Y. L. Shih, Y. L. Lo, C. F. Wang

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The mutagenicity of oxysterols, cholesterol-3β,5α,6β-triol (α-Triol), 7-keto-cholesterol (7-Keto) and cholesterol-5α,6α- epoxide (α-Epox) were examined by the Ames method and chromosome aberration test in this study. Only α-Triol concentration-dependently caused an increase of bacterial revertants in the absence of metabolic activating enzymes (S9), but not 7-keto and α-Epox. The mutagenic effect of α-Triol was reduced by the addition of S9. On the other hand, although α-Triol significantly induced chromosome aberration in CHO-K1 cells with and without S9. However, the addition of S9 reduced the degree of abnormal structure chromosome compared to without S9 mix. Catalase and superoxide dismutase (SOD) inhibited α-Triol induced increase of revertants in Salmonella typhimurium and chromosome aberration frequency in CHO cells, suggesting that reactive oxygen species (ROS) might be involved in the genotoxic effect of α-Triol. Treatment with α-Triol increased the ROS production in CHO cells, which could be attenuated by catalase and SOD. Results in this study suggested, for the first time that α-Triol, causes genotoxic effect in an ROS-dependent manner.

Original languageEnglish
Pages (from-to)617-622
Number of pages6
JournalFood and Chemical Toxicology
Volume43
Issue number4
DOIs
Publication statusPublished - Apr 2005

Fingerprint

chromosome aberrations
CHO Cells
genotoxicity
Chromosomes
Chromosome Aberrations
reactive oxygen species
Reactive Oxygen Species
Aberrations
cholesterol
Catalase
Superoxide Dismutase
catalase
superoxide dismutase
Cholesterol
Chromosome Structures
mutagenicity
cells
Salmonella typhimurium
epoxides
Salmonella Typhimurium

Keywords

  • Ames test
  • Antioxidant
  • Cholesterol-3β,5α,6β-triol
  • Chromosome aberration
  • Genotoxicity
  • Oxysterol
  • Reactive oxygen species

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Cholesterol-3-beta, 5-alpha, 6-beta-triol induced genotoxicity through reactive oxygen species formation. / Cheng, Y. W.; Kang, J. J.; Shih, Y. L.; Lo, Y. L.; Wang, C. F.

In: Food and Chemical Toxicology, Vol. 43, No. 4, 04.2005, p. 617-622.

Research output: Contribution to journalArticle

Cheng, Y. W. ; Kang, J. J. ; Shih, Y. L. ; Lo, Y. L. ; Wang, C. F. / Cholesterol-3-beta, 5-alpha, 6-beta-triol induced genotoxicity through reactive oxygen species formation. In: Food and Chemical Toxicology. 2005 ; Vol. 43, No. 4. pp. 617-622.
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