CHM-1 inhibits hepatocyte growth factor-induced invasion of SK-Hep-1 human hepatocellular carcinoma cells by suppressing matrix metalloproteinase-9 expression

Shih Wei Wang, Shiow Lin Pan, Chieh Yu Peng, Der Yi Huang, An Chi Tsai, Ya Ling Chang, Jih Hwa Guh, Sheng Chu Kuo, Kuo Hsiung Lee, Che Ming Teng

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Clinical observations suggest that hepatocyte growth factor (HGF) can promote invasion and metastasis in hepatocellular carcinoma. In this study, we found that HGF-stimulated invasion of SK-Hep-1 cells, together with increased expression of matrix metalloproteinase (MMP)-9. CHM-1 was identified from 2-phenyl-4-quinolone derivatives to potently inhibit HGF-induced cell invasion, proteolytic activity, and expression of MMP-9. CHM-1 significantly inhibited tyrosine autophosphorylation of c-Met induced by HGF. CHM-1 also suppressed HGF-induced Akt phosphorylation, and NF-κB activation, the downstream regulators of HGF/c-Met signaling, resulting in the inhibition of MMP-9. Thus, we suggest that CHM-1 is a potential therapeutic agent against tumor invasion.

Original languageEnglish
Pages (from-to)87-96
Number of pages10
JournalCancer Letters
Volume257
Issue number1
DOIs
Publication statusPublished - Nov 8 2007
Externally publishedYes

Fingerprint

Hepatocyte Growth Factor
Matrix Metalloproteinase 9
Hepatocellular Carcinoma
Tyrosine
Phosphorylation
Neoplasm Metastasis
Neoplasms

Keywords

  • Hepatocellular carcinoma
  • HGF
  • Invasion
  • MMP-9

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

CHM-1 inhibits hepatocyte growth factor-induced invasion of SK-Hep-1 human hepatocellular carcinoma cells by suppressing matrix metalloproteinase-9 expression. / Wang, Shih Wei; Pan, Shiow Lin; Peng, Chieh Yu; Huang, Der Yi; Tsai, An Chi; Chang, Ya Ling; Guh, Jih Hwa; Kuo, Sheng Chu; Lee, Kuo Hsiung; Teng, Che Ming.

In: Cancer Letters, Vol. 257, No. 1, 08.11.2007, p. 87-96.

Research output: Contribution to journalArticle

Wang, Shih Wei ; Pan, Shiow Lin ; Peng, Chieh Yu ; Huang, Der Yi ; Tsai, An Chi ; Chang, Ya Ling ; Guh, Jih Hwa ; Kuo, Sheng Chu ; Lee, Kuo Hsiung ; Teng, Che Ming. / CHM-1 inhibits hepatocyte growth factor-induced invasion of SK-Hep-1 human hepatocellular carcinoma cells by suppressing matrix metalloproteinase-9 expression. In: Cancer Letters. 2007 ; Vol. 257, No. 1. pp. 87-96.
@article{2407bbe85ef149ada25a92ebe2bf2349,
title = "CHM-1 inhibits hepatocyte growth factor-induced invasion of SK-Hep-1 human hepatocellular carcinoma cells by suppressing matrix metalloproteinase-9 expression",
abstract = "Clinical observations suggest that hepatocyte growth factor (HGF) can promote invasion and metastasis in hepatocellular carcinoma. In this study, we found that HGF-stimulated invasion of SK-Hep-1 cells, together with increased expression of matrix metalloproteinase (MMP)-9. CHM-1 was identified from 2-phenyl-4-quinolone derivatives to potently inhibit HGF-induced cell invasion, proteolytic activity, and expression of MMP-9. CHM-1 significantly inhibited tyrosine autophosphorylation of c-Met induced by HGF. CHM-1 also suppressed HGF-induced Akt phosphorylation, and NF-κB activation, the downstream regulators of HGF/c-Met signaling, resulting in the inhibition of MMP-9. Thus, we suggest that CHM-1 is a potential therapeutic agent against tumor invasion.",
keywords = "Hepatocellular carcinoma, HGF, Invasion, MMP-9",
author = "Wang, {Shih Wei} and Pan, {Shiow Lin} and Peng, {Chieh Yu} and Huang, {Der Yi} and Tsai, {An Chi} and Chang, {Ya Ling} and Guh, {Jih Hwa} and Kuo, {Sheng Chu} and Lee, {Kuo Hsiung} and Teng, {Che Ming}",
year = "2007",
month = "11",
day = "8",
doi = "10.1016/j.canlet.2007.07.002",
language = "English",
volume = "257",
pages = "87--96",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - CHM-1 inhibits hepatocyte growth factor-induced invasion of SK-Hep-1 human hepatocellular carcinoma cells by suppressing matrix metalloproteinase-9 expression

AU - Wang, Shih Wei

AU - Pan, Shiow Lin

AU - Peng, Chieh Yu

AU - Huang, Der Yi

AU - Tsai, An Chi

AU - Chang, Ya Ling

AU - Guh, Jih Hwa

AU - Kuo, Sheng Chu

AU - Lee, Kuo Hsiung

AU - Teng, Che Ming

PY - 2007/11/8

Y1 - 2007/11/8

N2 - Clinical observations suggest that hepatocyte growth factor (HGF) can promote invasion and metastasis in hepatocellular carcinoma. In this study, we found that HGF-stimulated invasion of SK-Hep-1 cells, together with increased expression of matrix metalloproteinase (MMP)-9. CHM-1 was identified from 2-phenyl-4-quinolone derivatives to potently inhibit HGF-induced cell invasion, proteolytic activity, and expression of MMP-9. CHM-1 significantly inhibited tyrosine autophosphorylation of c-Met induced by HGF. CHM-1 also suppressed HGF-induced Akt phosphorylation, and NF-κB activation, the downstream regulators of HGF/c-Met signaling, resulting in the inhibition of MMP-9. Thus, we suggest that CHM-1 is a potential therapeutic agent against tumor invasion.

AB - Clinical observations suggest that hepatocyte growth factor (HGF) can promote invasion and metastasis in hepatocellular carcinoma. In this study, we found that HGF-stimulated invasion of SK-Hep-1 cells, together with increased expression of matrix metalloproteinase (MMP)-9. CHM-1 was identified from 2-phenyl-4-quinolone derivatives to potently inhibit HGF-induced cell invasion, proteolytic activity, and expression of MMP-9. CHM-1 significantly inhibited tyrosine autophosphorylation of c-Met induced by HGF. CHM-1 also suppressed HGF-induced Akt phosphorylation, and NF-κB activation, the downstream regulators of HGF/c-Met signaling, resulting in the inhibition of MMP-9. Thus, we suggest that CHM-1 is a potential therapeutic agent against tumor invasion.

KW - Hepatocellular carcinoma

KW - HGF

KW - Invasion

KW - MMP-9

UR - http://www.scopus.com/inward/record.url?scp=34848825642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34848825642&partnerID=8YFLogxK

U2 - 10.1016/j.canlet.2007.07.002

DO - 10.1016/j.canlet.2007.07.002

M3 - Article

VL - 257

SP - 87

EP - 96

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -