Chitosan prevents the development of AOM-induced aberrant crypt foci in mice and suppressed the proliferation of AGS cells by inhibiting DNA synthesis

Shyr Yi Lin, Hing Yuen Chan, Fung Hsiu Shen, Mei Huei Chen, Ying Jan Wang, Chung Keung Yu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We study the effect of fungal-derived chitosan on the development of chemical-induced colonic precancerous lesions in ICR mice and delineate its possible molecular mechanisms. In the 2 weeks preventive experiments, mice fed with a diet containing high molecular weight chitosan (HMWC) had significant fewer aberrant crypt foci formation than those fed with control diet. As the treatment extended to 6 weeks, both low molecular weight chitosan (LMWC)- and HMWC-fed mice contained less aberrant crypt foci when compared to control. However, such effect was not observed in mice in the 6 weeks therapeutic experiments. The anti-tumorigenesis effect of water-soluble chitosan oligomer (WSCO) was tested on four cancer cell lines. WSCO significantly suppressed AGS and to a less extent, COLO 205 cells proliferation. Flow cytometry analysis of cell cycle distribution indicated that the percentage of S phase reduced significantly in AGS cells treated with WSCO together with a decrease in DNA synthesis rate in BrdU incorporation assay. WSCO treatment also upregulated cell cycle-related genes p21/Cip and p27/Kip, whereas downregulated that of PCNA.

Original languageEnglish
Pages (from-to)1573-1580
Number of pages8
JournalJournal of Cellular Biochemistry
Volume100
Issue number6
DOIs
Publication statusPublished - Apr 15 2007

Keywords

  • Anti-tumorigenicity
  • Cell cycle
  • Cells proliferation
  • Chitosan

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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