Abstract
We study the effect of fungal-derived chitosan on the development of chemical-induced colonic precancerous lesions in ICR mice and delineate its possible molecular mechanisms. In the 2 weeks preventive experiments, mice fed with a diet containing high molecular weight chitosan (HMWC) had significant fewer aberrant crypt foci formation than those fed with control diet. As the treatment extended to 6 weeks, both low molecular weight chitosan (LMWC)- and HMWC-fed mice contained less aberrant crypt foci when compared to control. However, such effect was not observed in mice in the 6 weeks therapeutic experiments. The anti-tumorigenesis effect of water-soluble chitosan oligomer (WSCO) was tested on four cancer cell lines. WSCO significantly suppressed AGS and to a less extent, COLO 205 cells proliferation. Flow cytometry analysis of cell cycle distribution indicated that the percentage of S phase reduced significantly in AGS cells treated with WSCO together with a decrease in DNA synthesis rate in BrdU incorporation assay. WSCO treatment also upregulated cell cycle-related genes p21/Cip and p27/Kip, whereas downregulated that of PCNA.
| Original language | English |
|---|---|
| Pages (from-to) | 1573-1580 |
| Number of pages | 8 |
| Journal | Journal of Cellular Biochemistry |
| Volume | 100 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Apr 15 2007 |
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Keywords
- Anti-tumorigenicity
- Cell cycle
- Cells proliferation
- Chitosan
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
Cite this
Chitosan prevents the development of AOM-induced aberrant crypt foci in mice and suppressed the proliferation of AGS cells by inhibiting DNA synthesis. / Lin, Shyr Yi; Chan, Hing Yuen; Shen, Fung Hsiu; Chen, Mei Huei; Wang, Ying Jan; Yu, Chung Keung.
In: Journal of Cellular Biochemistry, Vol. 100, No. 6, 15.04.2007, p. 1573-1580.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chitosan prevents the development of AOM-induced aberrant crypt foci in mice and suppressed the proliferation of AGS cells by inhibiting DNA synthesis
AU - Lin, Shyr Yi
AU - Chan, Hing Yuen
AU - Shen, Fung Hsiu
AU - Chen, Mei Huei
AU - Wang, Ying Jan
AU - Yu, Chung Keung
PY - 2007/4/15
Y1 - 2007/4/15
N2 - We study the effect of fungal-derived chitosan on the development of chemical-induced colonic precancerous lesions in ICR mice and delineate its possible molecular mechanisms. In the 2 weeks preventive experiments, mice fed with a diet containing high molecular weight chitosan (HMWC) had significant fewer aberrant crypt foci formation than those fed with control diet. As the treatment extended to 6 weeks, both low molecular weight chitosan (LMWC)- and HMWC-fed mice contained less aberrant crypt foci when compared to control. However, such effect was not observed in mice in the 6 weeks therapeutic experiments. The anti-tumorigenesis effect of water-soluble chitosan oligomer (WSCO) was tested on four cancer cell lines. WSCO significantly suppressed AGS and to a less extent, COLO 205 cells proliferation. Flow cytometry analysis of cell cycle distribution indicated that the percentage of S phase reduced significantly in AGS cells treated with WSCO together with a decrease in DNA synthesis rate in BrdU incorporation assay. WSCO treatment also upregulated cell cycle-related genes p21/Cip and p27/Kip, whereas downregulated that of PCNA.
AB - We study the effect of fungal-derived chitosan on the development of chemical-induced colonic precancerous lesions in ICR mice and delineate its possible molecular mechanisms. In the 2 weeks preventive experiments, mice fed with a diet containing high molecular weight chitosan (HMWC) had significant fewer aberrant crypt foci formation than those fed with control diet. As the treatment extended to 6 weeks, both low molecular weight chitosan (LMWC)- and HMWC-fed mice contained less aberrant crypt foci when compared to control. However, such effect was not observed in mice in the 6 weeks therapeutic experiments. The anti-tumorigenesis effect of water-soluble chitosan oligomer (WSCO) was tested on four cancer cell lines. WSCO significantly suppressed AGS and to a less extent, COLO 205 cells proliferation. Flow cytometry analysis of cell cycle distribution indicated that the percentage of S phase reduced significantly in AGS cells treated with WSCO together with a decrease in DNA synthesis rate in BrdU incorporation assay. WSCO treatment also upregulated cell cycle-related genes p21/Cip and p27/Kip, whereas downregulated that of PCNA.
KW - Anti-tumorigenicity
KW - Cell cycle
KW - Cells proliferation
KW - Chitosan
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UR - http://www.scopus.com/inward/citedby.url?scp=34047097273&partnerID=8YFLogxK
U2 - 10.1002/jcb.21152
DO - 10.1002/jcb.21152
M3 - Article
VL - 100
SP - 1573
EP - 1580
JO - Journal of Cellular Biochemistry
JF - Journal of Cellular Biochemistry
SN - 0730-2312
IS - 6
ER -