Chitosan nanoparticles for antimicrobial photodynamic inactivation: Characterization and in vitro investigation

Chueh Pin Chen, Chin Tin Chen, Tsuimin Tsai

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The growing resistance to antibiotics has rendered antimicrobial photodynamic inactivation (PDI) an attractive alternative treatment modality for infectious diseases. Chitosan (CS) was shown to further potentiate the PDI effect of photosensitizers and was therefore used in this study to investigate its ability to potentiate the activity of erythrosine (ER) against bacteria and yeast. CS nanoparticles loaded with ER were prepared by ionic gelation method and tested for their PDI efficacy on planktonic cells and biofilms of Streptococcus mutans, Pseudomonas aeruginosa and Candida albicans. The nanoparticles were characterized for their size, polydispersity index and zeta potential. No toxicity was observed when planktonic cells and biofilms were treated with the nanoparticles in the dark. However, when the cells were exposed to light irradiation after treatment with free ER or ER/CS nanoparticles, a significant phototoxicity was observed. The antimicrobial activity of ER/CS nanoparticles was significantly higher than ER in free form. The particle size and incubation time of the nanoparticles also appeared to be important factors affecting their PDI activity against S. mutans and C. albicans. Erythrosine (ER)-mediated photodynamic inactivation (PDI) was able to kill microorganisms. Chitosan nanoparticles loaded with ER exhibited better PDI efficacy than ER alone on microbial cultures and biofilms.

Original languageEnglish
Pages (from-to)570-576
Number of pages7
JournalPhotochemistry and Photobiology
Volume88
Issue number3
DOIs
Publication statusPublished - May 2012

Fingerprint

Erythrosine
Chitosan
deactivation
Nanoparticles
nanoparticles
biofilms
Biofilms
Streptococcus mutans
Candida albicans
cells
streptococcus
pseudomonas
antibiotics
yeast
gelation
infectious diseases
microorganisms
Phototoxic Dermatitis
In Vitro Techniques
toxicity

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

Chitosan nanoparticles for antimicrobial photodynamic inactivation : Characterization and in vitro investigation. / Chen, Chueh Pin; Chen, Chin Tin; Tsai, Tsuimin.

In: Photochemistry and Photobiology, Vol. 88, No. 3, 05.2012, p. 570-576.

Research output: Contribution to journalArticle

@article{504b57a995d840bb85b00bedf7def319,
title = "Chitosan nanoparticles for antimicrobial photodynamic inactivation: Characterization and in vitro investigation",
abstract = "The growing resistance to antibiotics has rendered antimicrobial photodynamic inactivation (PDI) an attractive alternative treatment modality for infectious diseases. Chitosan (CS) was shown to further potentiate the PDI effect of photosensitizers and was therefore used in this study to investigate its ability to potentiate the activity of erythrosine (ER) against bacteria and yeast. CS nanoparticles loaded with ER were prepared by ionic gelation method and tested for their PDI efficacy on planktonic cells and biofilms of Streptococcus mutans, Pseudomonas aeruginosa and Candida albicans. The nanoparticles were characterized for their size, polydispersity index and zeta potential. No toxicity was observed when planktonic cells and biofilms were treated with the nanoparticles in the dark. However, when the cells were exposed to light irradiation after treatment with free ER or ER/CS nanoparticles, a significant phototoxicity was observed. The antimicrobial activity of ER/CS nanoparticles was significantly higher than ER in free form. The particle size and incubation time of the nanoparticles also appeared to be important factors affecting their PDI activity against S. mutans and C. albicans. Erythrosine (ER)-mediated photodynamic inactivation (PDI) was able to kill microorganisms. Chitosan nanoparticles loaded with ER exhibited better PDI efficacy than ER alone on microbial cultures and biofilms.",
author = "Chen, {Chueh Pin} and Chen, {Chin Tin} and Tsuimin Tsai",
year = "2012",
month = "5",
doi = "10.1111/j.1751-1097.2012.01101.x",
language = "English",
volume = "88",
pages = "570--576",
journal = "Photochemistry and Photobiology",
issn = "0031-8655",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Chitosan nanoparticles for antimicrobial photodynamic inactivation

T2 - Characterization and in vitro investigation

AU - Chen, Chueh Pin

AU - Chen, Chin Tin

AU - Tsai, Tsuimin

PY - 2012/5

Y1 - 2012/5

N2 - The growing resistance to antibiotics has rendered antimicrobial photodynamic inactivation (PDI) an attractive alternative treatment modality for infectious diseases. Chitosan (CS) was shown to further potentiate the PDI effect of photosensitizers and was therefore used in this study to investigate its ability to potentiate the activity of erythrosine (ER) against bacteria and yeast. CS nanoparticles loaded with ER were prepared by ionic gelation method and tested for their PDI efficacy on planktonic cells and biofilms of Streptococcus mutans, Pseudomonas aeruginosa and Candida albicans. The nanoparticles were characterized for their size, polydispersity index and zeta potential. No toxicity was observed when planktonic cells and biofilms were treated with the nanoparticles in the dark. However, when the cells were exposed to light irradiation after treatment with free ER or ER/CS nanoparticles, a significant phototoxicity was observed. The antimicrobial activity of ER/CS nanoparticles was significantly higher than ER in free form. The particle size and incubation time of the nanoparticles also appeared to be important factors affecting their PDI activity against S. mutans and C. albicans. Erythrosine (ER)-mediated photodynamic inactivation (PDI) was able to kill microorganisms. Chitosan nanoparticles loaded with ER exhibited better PDI efficacy than ER alone on microbial cultures and biofilms.

AB - The growing resistance to antibiotics has rendered antimicrobial photodynamic inactivation (PDI) an attractive alternative treatment modality for infectious diseases. Chitosan (CS) was shown to further potentiate the PDI effect of photosensitizers and was therefore used in this study to investigate its ability to potentiate the activity of erythrosine (ER) against bacteria and yeast. CS nanoparticles loaded with ER were prepared by ionic gelation method and tested for their PDI efficacy on planktonic cells and biofilms of Streptococcus mutans, Pseudomonas aeruginosa and Candida albicans. The nanoparticles were characterized for their size, polydispersity index and zeta potential. No toxicity was observed when planktonic cells and biofilms were treated with the nanoparticles in the dark. However, when the cells were exposed to light irradiation after treatment with free ER or ER/CS nanoparticles, a significant phototoxicity was observed. The antimicrobial activity of ER/CS nanoparticles was significantly higher than ER in free form. The particle size and incubation time of the nanoparticles also appeared to be important factors affecting their PDI activity against S. mutans and C. albicans. Erythrosine (ER)-mediated photodynamic inactivation (PDI) was able to kill microorganisms. Chitosan nanoparticles loaded with ER exhibited better PDI efficacy than ER alone on microbial cultures and biofilms.

UR - http://www.scopus.com/inward/record.url?scp=84862777740&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862777740&partnerID=8YFLogxK

U2 - 10.1111/j.1751-1097.2012.01101.x

DO - 10.1111/j.1751-1097.2012.01101.x

M3 - Article

C2 - 22283820

AN - SCOPUS:84862777740

VL - 88

SP - 570

EP - 576

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 0031-8655

IS - 3

ER -