Chitosan augments photodynamic inactivation of gram-positive and gram-negative bacteria

Tsui-Min Tsai, Hsiung-Fei Chien, Tze Hsien Wang, Ching Tsan Huang, Yaw Bee Ker, Chin Tin Chen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Antimicrobial photodynamic inactivation (PDI) was shown to be a promising treatment modality for microbial infections. This study explores the effect of chitosan, a polycationic biopolymer, in increasing the PDI efficacy against Gram-positive bacteria, including Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, and methicillin-resistant S. aureus (MRSA), as well as the Gram-negative bacteria Pseudomonas aeruginosa and Acinetobacter baumannii. Chitosan at 8 CFU/ml). Similar results were also found with toluidine blue O (TBO)-mediated PDI in planktonic and biofilm cells. However, without PDI treatment, chitosan alone did not exert significant antimicrobial activity with 30 min of incubation, suggesting that the potentiated effect of chitosan worked after the bacterial damage induced by PDI. Further studies indicated that the potentiated PDI effect of chitosan was related to the level of PDI damage and the deacetylation level of the chitosan. These results indicate that the combination of PDI and chitosan is quite promising for eradicating microbial infections.

Original languageEnglish
Pages (from-to)1883-1890
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume55
Issue number5
DOIs
Publication statusPublished - May 2011

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Chitosan
Gram-Negative Bacteria
Acinetobacter baumannii
Tolonium Chloride
Biopolymers
Staphylococcus epidermidis
Streptococcus pyogenes
Gram-Positive Bacteria
Methicillin-Resistant Staphylococcus aureus
Biofilms
Infection
Pseudomonas aeruginosa
Staphylococcus aureus

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

Cite this

Chitosan augments photodynamic inactivation of gram-positive and gram-negative bacteria. / Tsai, Tsui-Min; Chien, Hsiung-Fei; Wang, Tze Hsien; Huang, Ching Tsan; Ker, Yaw Bee; Chen, Chin Tin.

In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 5, 05.2011, p. 1883-1890.

Research output: Contribution to journalArticle

Tsai, Tsui-Min ; Chien, Hsiung-Fei ; Wang, Tze Hsien ; Huang, Ching Tsan ; Ker, Yaw Bee ; Chen, Chin Tin. / Chitosan augments photodynamic inactivation of gram-positive and gram-negative bacteria. In: Antimicrobial Agents and Chemotherapy. 2011 ; Vol. 55, No. 5. pp. 1883-1890.
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