CHIP attenuates lipopolysaccharide-induced cardiac hypertrophy and apoptosis by promoting NFATc3 proteasomal degradation

Chun Nun Chao, Chao Hung Lai, Khan Farheen Badrealam, Jeng Fan Lo, Chia Yao Shen, Chia Hua Chen, Ray Jade Chen, Vijaya Padma Viswanadha, Wei Wen Kuo, Chih Yang Huang

Research output: Contribution to journalArticle

Abstract

Carboxyl-terminus of Hsc70 interacting protein (CHIP) is a chaperone-dependent E3-ubiquitin ligase with important function in protein quality control system. In the current research endeavor, we have investigated the putative role of CHIP in lipopolysaccharides (LPS)-induced cardiomyopathies. Basically, H9c2 cardiomyoblasts were transfected with CHIP for 24 hr, and thereafter, treated with LPS for 12 hr. Concomitantly, western blot analysis, actin staining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, and coimmunoprecipitation studies were performed to investigate the underlying intricacies. Interestingly, western blot analysis revealed that the expression of hypertrophy and apoptosis-related proteins were considerably reduced following overexpression of CHIP. Moreover, Actin staining and TUNEL assay further ascertained the attenuation of cardiac hypertrophy and apoptosis following overexpression of CHIP respectively. These aspects instigate the role of CHIP in attenuation of LPS-induced cardiomyopathies. Additionally and importantly, co-immunoprecipitation and western blot studies revealed that CHIP plausibly promotes degradation of nuclear factor of activated T cells 3 (NFATc3) through ubiquitin-proteasomal pathway. Taken together, our study reveals that CHIP attenuates LPS-induced cardiac hypertrophy and apoptosis perhaps by promoting NFATc3 proteasomal degradation.

Original languageEnglish
Pages (from-to)20128-20138
Number of pages11
JournalJournal of Cellular Physiology
Volume234
Issue number11
DOIs
Publication statusPublished - Nov 1 2019

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HSC70 Heat-Shock Proteins
NFATC Transcription Factors
Cardiomegaly
Lipopolysaccharides
Apoptosis
Degradation
Proteins
Western Blotting
Cardiomyopathies
Actins
Labeling
Staining and Labeling
Assays
Ubiquitin-Protein Ligases
DNA Nucleotidylexotransferase
Transferases
Ubiquitin
Immunoprecipitation
Quality Control
Hypertrophy

Keywords

  • apoptosis
  • CHIP
  • hypertrophy
  • lipopolysaccharide
  • NFATc3

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Chao, C. N., Lai, C. H., Badrealam, K. F., Lo, J. F., Shen, C. Y., Chen, C. H., ... Huang, C. Y. (2019). CHIP attenuates lipopolysaccharide-induced cardiac hypertrophy and apoptosis by promoting NFATc3 proteasomal degradation. Journal of Cellular Physiology, 234(11), 20128-20138. https://doi.org/10.1002/jcp.28614

CHIP attenuates lipopolysaccharide-induced cardiac hypertrophy and apoptosis by promoting NFATc3 proteasomal degradation. / Chao, Chun Nun; Lai, Chao Hung; Badrealam, Khan Farheen; Lo, Jeng Fan; Shen, Chia Yao; Chen, Chia Hua; Chen, Ray Jade; Viswanadha, Vijaya Padma; Kuo, Wei Wen; Huang, Chih Yang.

In: Journal of Cellular Physiology, Vol. 234, No. 11, 01.11.2019, p. 20128-20138.

Research output: Contribution to journalArticle

Chao, CN, Lai, CH, Badrealam, KF, Lo, JF, Shen, CY, Chen, CH, Chen, RJ, Viswanadha, VP, Kuo, WW & Huang, CY 2019, 'CHIP attenuates lipopolysaccharide-induced cardiac hypertrophy and apoptosis by promoting NFATc3 proteasomal degradation', Journal of Cellular Physiology, vol. 234, no. 11, pp. 20128-20138. https://doi.org/10.1002/jcp.28614
Chao, Chun Nun ; Lai, Chao Hung ; Badrealam, Khan Farheen ; Lo, Jeng Fan ; Shen, Chia Yao ; Chen, Chia Hua ; Chen, Ray Jade ; Viswanadha, Vijaya Padma ; Kuo, Wei Wen ; Huang, Chih Yang. / CHIP attenuates lipopolysaccharide-induced cardiac hypertrophy and apoptosis by promoting NFATc3 proteasomal degradation. In: Journal of Cellular Physiology. 2019 ; Vol. 234, No. 11. pp. 20128-20138.
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