Chinese Herbal Mixture, Tien-Hsien Liquid, Induces G2/M Cycle Arrest and Radiosensitivity in MCF-7 Human Breast Cancer Cells through Mechanisms Involving DNMT1 and Rad51 Downregulation

Chih Jung Yao, Jyh Ming Chow, Chia Ming Yang, Hui Ching Kuo, Chia Lun Chang, Hsin Lun Lee, I. Chun Lai, Shuang En Chuang, Gi Ming Lai

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3 Citations (Scopus)

Abstract

The Chinese herbal mixture, Tien-Hsien Liquid (THL), has been proven to suppress the growth and invasiveness of cancer cells and is currently regarded as a complementary medicine for the treatment of cancer. Our previous study using acute promyelocytic leukemia cells uncovered its effect on the downregulation of DNA methyltransferase 1 (DNMT1) which is often overexpressed in cancer cells resulting in the repression of tumor suppressors via hypermethylation. Herein, we explored the effects of THL in MCF-7 breast cancer cells that also demonstrate elevated DNMT1. The results show that THL dose-dependently downregulated DNMT1 accompanied by the induction of tumor suppressors such as p21 and p15. THL arrested cell cycle in G2/M phase and decreased the protein levels of cyclin A, cyclin B1, phospho-pRb, and AKT. DNMT1 inhibition was previously reported to exert a radiosensitizing effect in cancer cells through the repression of DNA repair. We found that THL enhanced radiation-induced clonogenic cell death in MCF-7 cells and decreased the level of DNA double-strand break repair protein, Rad51. Our observations may be the result of DNMT1 downregulation. Due to the fact that DNMT1 inhibition is now a mainstream strategy for anticancer therapy, further clinical trials of THL to confirm its clinical efficacy are warranted.

Original languageEnglish
Article number3251046
JournalEvidence-based Complementary and Alternative Medicine
Volume2016
DOIs
Publication statusPublished - Jan 1 2016

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Radiation Tolerance
Methyltransferases
Down-Regulation
Breast Neoplasms
DNA
Neoplasms
Radiation-Sensitizing Agents
Cyclin B1
Cyclin A
Acute Promyelocytic Leukemia
Double-Stranded DNA Breaks
G2 Phase
MCF-7 Cells
Complementary Therapies
DNA Repair
Cell Division
tien-hsien liquid
Cell Cycle
Proteins
Cell Death

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

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title = "Chinese Herbal Mixture, Tien-Hsien Liquid, Induces G2/M Cycle Arrest and Radiosensitivity in MCF-7 Human Breast Cancer Cells through Mechanisms Involving DNMT1 and Rad51 Downregulation",
abstract = "The Chinese herbal mixture, Tien-Hsien Liquid (THL), has been proven to suppress the growth and invasiveness of cancer cells and is currently regarded as a complementary medicine for the treatment of cancer. Our previous study using acute promyelocytic leukemia cells uncovered its effect on the downregulation of DNA methyltransferase 1 (DNMT1) which is often overexpressed in cancer cells resulting in the repression of tumor suppressors via hypermethylation. Herein, we explored the effects of THL in MCF-7 breast cancer cells that also demonstrate elevated DNMT1. The results show that THL dose-dependently downregulated DNMT1 accompanied by the induction of tumor suppressors such as p21 and p15. THL arrested cell cycle in G2/M phase and decreased the protein levels of cyclin A, cyclin B1, phospho-pRb, and AKT. DNMT1 inhibition was previously reported to exert a radiosensitizing effect in cancer cells through the repression of DNA repair. We found that THL enhanced radiation-induced clonogenic cell death in MCF-7 cells and decreased the level of DNA double-strand break repair protein, Rad51. Our observations may be the result of DNMT1 downregulation. Due to the fact that DNMT1 inhibition is now a mainstream strategy for anticancer therapy, further clinical trials of THL to confirm its clinical efficacy are warranted.",
author = "Yao, {Chih Jung} and Chow, {Jyh Ming} and Yang, {Chia Ming} and Kuo, {Hui Ching} and Chang, {Chia Lun} and Lee, {Hsin Lun} and Lai, {I. Chun} and Chuang, {Shuang En} and Lai, {Gi Ming}",
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AU - Yao, Chih Jung

AU - Chow, Jyh Ming

AU - Yang, Chia Ming

AU - Kuo, Hui Ching

AU - Chang, Chia Lun

AU - Lee, Hsin Lun

AU - Lai, I. Chun

AU - Chuang, Shuang En

AU - Lai, Gi Ming

PY - 2016/1/1

Y1 - 2016/1/1

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