Chemical-free and reusable cellular analysis: Electrochemical impedance spectroscopy with a transparent ITO culture chip

Sheng Yi Hsiao, Dai Chian Chen, Chih Hui Yang, Haw Ming Huang, Yen Pei Lu, Hui Shun Huang, Chun Yen Lin, Yung Sheng Lin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To advance innovative green technology in studying cytology, this study developed an electrochemical impedance spectroscopic (EIS) system with an indium tin oxide (ITO) culture chip module. This paper also demonstrates typical examples of solution effects and B16-F10 cell culture. Results indicate that higher concentrations of saline or albumin had lower impedance. From impedance data, cell proliferation and decline could be elucidated. The impedance soon decreasedwhen Triton X-100 was applied to kill cells. Furthermore, the implemented transparent ITO culture chip module is experiment-friendly to perform optical inspections. The proposed green EIS system which is advantage of chemical-free and reusability can be widely applied to cytology studies in the future.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalInternational Journal of Technology and Human Interaction
Volume8
Issue number3
DOIs
Publication statusPublished - Jul 2012

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Keywords

  • B16-F10 (Murine melanoma cell line)
  • Cell
  • Chip
  • Impedance
  • Indium tin oxide

ASJC Scopus subject areas

  • Human-Computer Interaction
  • Information Systems

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