Characterization of Wolbachia postica, the cause of reproductive incompatibility among alfalfa weevil strains

Sy Jye Christine Leu, Joseph K K Li, Ting H. Hsiao

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Wolbachia postica, which causes reproductive incompatibility between male western alfalfa weevils and female eastern or Egyptian weevils, was isolated by Renografin density gradients. Hybridization of DNA from this isolated microorganism with a mycoplasma-specific gene probe confirmed it is not a mycoplasma but a wolbachia. Polyclonal antibodies produced against inactivated Wolbachia postica showed high sensitivity in fluorescent light microscopy and immunoelectron microscopy. This antibody was specific for Wolbachia-infected adult weevil by immunoblots which were reliable and reproducible, and more sensitive than the Giemsa stain procedure. A 135-kDa protein detected in all weevils infected with wolbachiae appeared to be a good marker for the microorganism. This technique facilitates the detection of wolbachiae-infected male weevils. Dissection of the ovary was not required to analyze the female weevils.

Original languageEnglish
Pages (from-to)248-259
Number of pages12
JournalJournal of Invertebrate Pathology
Volume54
Issue number2
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Hypera postica
Wolbachia
incompatibility
alfalfa
Curculionidae
antibody
microscopy
microorganism
dissection
Mycoplasma
probe
DNA
protein
gene
microorganisms
fluorescent lighting
polyclonal antibodies
nucleic acid hybridization
probes (equipment)
light microscopy

Keywords

  • alfalfa weevil strains
  • fluorescent microscopy
  • H. brunneipennis
  • Hypera postica
  • immunoblotting
  • immunoelectron microscopy
  • polyclonal antibody
  • Renografin density gradient
  • reproductive incompatibility
  • rickettsia
  • wolbachia
  • Wolbachia postica

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

Characterization of Wolbachia postica, the cause of reproductive incompatibility among alfalfa weevil strains. / Leu, Sy Jye Christine; Li, Joseph K K; Hsiao, Ting H.

In: Journal of Invertebrate Pathology, Vol. 54, No. 2, 1989, p. 248-259.

Research output: Contribution to journalArticle

@article{2ec730aa87eb476b98a5ae864b4bf61f,
title = "Characterization of Wolbachia postica, the cause of reproductive incompatibility among alfalfa weevil strains",
abstract = "Wolbachia postica, which causes reproductive incompatibility between male western alfalfa weevils and female eastern or Egyptian weevils, was isolated by Renografin density gradients. Hybridization of DNA from this isolated microorganism with a mycoplasma-specific gene probe confirmed it is not a mycoplasma but a wolbachia. Polyclonal antibodies produced against inactivated Wolbachia postica showed high sensitivity in fluorescent light microscopy and immunoelectron microscopy. This antibody was specific for Wolbachia-infected adult weevil by immunoblots which were reliable and reproducible, and more sensitive than the Giemsa stain procedure. A 135-kDa protein detected in all weevils infected with wolbachiae appeared to be a good marker for the microorganism. This technique facilitates the detection of wolbachiae-infected male weevils. Dissection of the ovary was not required to analyze the female weevils.",
keywords = "alfalfa weevil strains, fluorescent microscopy, H. brunneipennis, Hypera postica, immunoblotting, immunoelectron microscopy, polyclonal antibody, Renografin density gradient, reproductive incompatibility, rickettsia, wolbachia, Wolbachia postica",
author = "Leu, {Sy Jye Christine} and Li, {Joseph K K} and Hsiao, {Ting H.}",
year = "1989",
doi = "10.1016/0022-2011(89)90035-9",
language = "English",
volume = "54",
pages = "248--259",
journal = "Journal of Invertebrate Pathology",
issn = "0022-2011",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Characterization of Wolbachia postica, the cause of reproductive incompatibility among alfalfa weevil strains

AU - Leu, Sy Jye Christine

AU - Li, Joseph K K

AU - Hsiao, Ting H.

PY - 1989

Y1 - 1989

N2 - Wolbachia postica, which causes reproductive incompatibility between male western alfalfa weevils and female eastern or Egyptian weevils, was isolated by Renografin density gradients. Hybridization of DNA from this isolated microorganism with a mycoplasma-specific gene probe confirmed it is not a mycoplasma but a wolbachia. Polyclonal antibodies produced against inactivated Wolbachia postica showed high sensitivity in fluorescent light microscopy and immunoelectron microscopy. This antibody was specific for Wolbachia-infected adult weevil by immunoblots which were reliable and reproducible, and more sensitive than the Giemsa stain procedure. A 135-kDa protein detected in all weevils infected with wolbachiae appeared to be a good marker for the microorganism. This technique facilitates the detection of wolbachiae-infected male weevils. Dissection of the ovary was not required to analyze the female weevils.

AB - Wolbachia postica, which causes reproductive incompatibility between male western alfalfa weevils and female eastern or Egyptian weevils, was isolated by Renografin density gradients. Hybridization of DNA from this isolated microorganism with a mycoplasma-specific gene probe confirmed it is not a mycoplasma but a wolbachia. Polyclonal antibodies produced against inactivated Wolbachia postica showed high sensitivity in fluorescent light microscopy and immunoelectron microscopy. This antibody was specific for Wolbachia-infected adult weevil by immunoblots which were reliable and reproducible, and more sensitive than the Giemsa stain procedure. A 135-kDa protein detected in all weevils infected with wolbachiae appeared to be a good marker for the microorganism. This technique facilitates the detection of wolbachiae-infected male weevils. Dissection of the ovary was not required to analyze the female weevils.

KW - alfalfa weevil strains

KW - fluorescent microscopy

KW - H. brunneipennis

KW - Hypera postica

KW - immunoblotting

KW - immunoelectron microscopy

KW - polyclonal antibody

KW - Renografin density gradient

KW - reproductive incompatibility

KW - rickettsia

KW - wolbachia

KW - Wolbachia postica

UR - http://www.scopus.com/inward/record.url?scp=38249025371&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38249025371&partnerID=8YFLogxK

U2 - 10.1016/0022-2011(89)90035-9

DO - 10.1016/0022-2011(89)90035-9

M3 - Article

AN - SCOPUS:38249025371

VL - 54

SP - 248

EP - 259

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

SN - 0022-2011

IS - 2

ER -