Characterization of two mosquito STATs, AaSTAT and CtSTAT: Differential regulation of tyrosine phosphorylation and DNA binding activity by lipopolysaccharide treatment and by Japanese encephalitis virus infection

Chang Chi Lin, Chih Ming Chou, Ya Li Hsu, Jih Ching Lien, Yu Ming Wang, Shui Tsung Chen, Shu Chuan Tsai, Pei Wen Hsiao, Chang Jen Huang

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Abstract

Two mosquito STATs, AaSTAT and CtSTAT, have been cloned from Aedes albopictus and Culex tritaeniorhynchus mosquitoes, respectively. These two STATs are more similar to those of Drosophila, Anopheles, and mammalian STAT5 in the DNA binding and Src homology 2 domains. The mRNA transcripts are expressed at all developmental stages, and the proteins are present predominantly at the pupal and adult stages in both mosquitoes. Stimulation with lipopolysaccharide resulted in an increase of tyrosine phosphorylation and DNA binding activity of AaSTAT and CtSTAT as well as an increase of luciferase activity of a reporter gene containing Drosophila STAT binding motif in mosquito C6/36 cells. After being infected with Japanese encephalitis virus, nuclear extracts of C6/36 cells revealed a decrease of tyrosine phosphorylation and DNA binding activity of AaSTAT which could be restored by sodium orthovanadate treatment. Taking all of the data together, this is the first report to clone and characterize two mosquito STATs with 81% identity and to demonstrate a different response of tyrosine phosphorylation and DNA binding of these two STATs by lipopolysaccharide treatment and by Japanese encephalitis virus infection.

Original languageEnglish
Pages (from-to)3308-3317
Number of pages10
JournalJournal of Biological Chemistry
Volume279
Issue number5
DOIs
Publication statusPublished - Jan 30 2004

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Japanese Encephalitis Virus
Phosphorylation
Culicidae
Viruses
Tyrosine
Lipopolysaccharides
DNA
Infection
Drosophila
Vanadates
Luciferases
Culex
Anopheles
src Homology Domains
Aedes
Reporter Genes
Genes
Sodium
Messenger RNA
Clone Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of two mosquito STATs, AaSTAT and CtSTAT : Differential regulation of tyrosine phosphorylation and DNA binding activity by lipopolysaccharide treatment and by Japanese encephalitis virus infection. / Lin, Chang Chi; Chou, Chih Ming; Hsu, Ya Li; Lien, Jih Ching; Wang, Yu Ming; Chen, Shui Tsung; Tsai, Shu Chuan; Hsiao, Pei Wen; Huang, Chang Jen.

In: Journal of Biological Chemistry, Vol. 279, No. 5, 30.01.2004, p. 3308-3317.

Research output: Contribution to journalArticle

Lin, Chang Chi ; Chou, Chih Ming ; Hsu, Ya Li ; Lien, Jih Ching ; Wang, Yu Ming ; Chen, Shui Tsung ; Tsai, Shu Chuan ; Hsiao, Pei Wen ; Huang, Chang Jen. / Characterization of two mosquito STATs, AaSTAT and CtSTAT : Differential regulation of tyrosine phosphorylation and DNA binding activity by lipopolysaccharide treatment and by Japanese encephalitis virus infection. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 5. pp. 3308-3317.
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