Characterization of the subunit structure of gonadotropin receptor in luteinized rat ovary

J. Hwang, K. M J Menon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Gonadotropin receptors with specificity, high affinity and low capacity for luteinizing hormone and human chorionic gonadotropin (hCG) have been identified in rat luteal cells. To investigate the nature of the receptor, we have employed disuccinimidyl suberate, a cross-linker noncleavable by reducing agents, and dithiobis(succinimidyl propionate), a cleavable cross-linker, to covalently cross-link the 125I-hCG·receptor complex. The molecular weight of 125I-hCG-linked receptor complex and the receptor subunit structure were determined by electrophoresis in either 10 or 4.5% acrylamide in the presence of 0.1% sodium dodecyl sulfate with or without reducing agents. Autoradiographic analysis of the 125I-hCG-linked receptor separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing condition revealed a single labeled band corresponding to M(r) = 305,000 ± 15,000. However, electrophoresis performed in the presence of 50 nM dithiothreitol and 2% β-mercaptoethanol resulted in the appearance of four labeled bands corresponding to M(r) = 105,000 ± 4,000, 96,000 ± 5,000, 74,000 ± 4,000, and 62,000 ± 4,000 concomitant with the loss of the labeled band in the M(r) = 305,000 region. Further experiments demonstrated that these four labeled bands were derived from the same molecular species. In addition, the 125I-hCG-linked receptor in the absence of reducing agent was not dissociated into subunits even by treatment with strong denaturing agent (8 M urea). The appearance of the cross-linked 125I-hCG·receptor was effectively inhibited by the unlabeled β-subunit of hCG, intact hCG, and luteinizing hormone and partially inhibited by the α-subunit of hCG but not by choleratoxin, gonadotropin-releasing hormone, insulin or bovine serum albumin. These data suggest that 1) the hCG/luteinizing hormone receptor is an oligomeric complex linked by disulfide bonds and 2) that under reducing conditions, the oligomeric receptor dissociates into four nonidentical subunits.

Original languageEnglish
Pages (from-to)1978-1985
Number of pages8
JournalJournal of Biological Chemistry
Volume259
Issue number3
Publication statusPublished - 1984
Externally publishedYes

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Gonadotropin Receptors
Chorionic Gonadotropin
LH Receptors
Rats
Ovary
Reducing Agents
Luteinizing Hormone
Sodium Dodecyl Sulfate
Electrophoresis
Luteal Cells
Mercaptoethanol
Acrylamide
Dithiothreitol
Bovine Serum Albumin
Gonadotropin-Releasing Hormone
Disulfides
Urea
Polyacrylamide Gel Electrophoresis
Molecular Weight
Insulin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of the subunit structure of gonadotropin receptor in luteinized rat ovary. / Hwang, J.; Menon, K. M J.

In: Journal of Biological Chemistry, Vol. 259, No. 3, 1984, p. 1978-1985.

Research output: Contribution to journalArticle

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abstract = "Gonadotropin receptors with specificity, high affinity and low capacity for luteinizing hormone and human chorionic gonadotropin (hCG) have been identified in rat luteal cells. To investigate the nature of the receptor, we have employed disuccinimidyl suberate, a cross-linker noncleavable by reducing agents, and dithiobis(succinimidyl propionate), a cleavable cross-linker, to covalently cross-link the 125I-hCG·receptor complex. The molecular weight of 125I-hCG-linked receptor complex and the receptor subunit structure were determined by electrophoresis in either 10 or 4.5{\%} acrylamide in the presence of 0.1{\%} sodium dodecyl sulfate with or without reducing agents. Autoradiographic analysis of the 125I-hCG-linked receptor separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing condition revealed a single labeled band corresponding to M(r) = 305,000 ± 15,000. However, electrophoresis performed in the presence of 50 nM dithiothreitol and 2{\%} β-mercaptoethanol resulted in the appearance of four labeled bands corresponding to M(r) = 105,000 ± 4,000, 96,000 ± 5,000, 74,000 ± 4,000, and 62,000 ± 4,000 concomitant with the loss of the labeled band in the M(r) = 305,000 region. Further experiments demonstrated that these four labeled bands were derived from the same molecular species. In addition, the 125I-hCG-linked receptor in the absence of reducing agent was not dissociated into subunits even by treatment with strong denaturing agent (8 M urea). The appearance of the cross-linked 125I-hCG·receptor was effectively inhibited by the unlabeled β-subunit of hCG, intact hCG, and luteinizing hormone and partially inhibited by the α-subunit of hCG but not by choleratoxin, gonadotropin-releasing hormone, insulin or bovine serum albumin. These data suggest that 1) the hCG/luteinizing hormone receptor is an oligomeric complex linked by disulfide bonds and 2) that under reducing conditions, the oligomeric receptor dissociates into four nonidentical subunits.",
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