Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene

Cheng Ming Lee, Yi Ping Shih, Cho Han Wu, Yi Ming Arthur Chen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Glycine N-methyltransferase (GNMT) is a tumor susceptibility gene for both hepatocellular carcinoma and prostate cancer. We have previously characterized GNMT genomic structure and mapped its chromosomal localization to 6p12. For this study we identified a GNMT transcriptional start site at the 14th position upstream of the ATG codon. Electrophoretic mobility shift assay results indicate binding of the nuclear factor-Y (NF-Y) transcription factor to the CCAAT box (- 71/- 67) of the GNMT gene. Mutation assay results suggest that the nucleotide sequence in the - 56/- 47 region is a binding site for a putative transcriptional factor. The TATA-less core promoter (- 133/+ 14) contains three major elements: an Sp1 site, CCAAT box, and a novel box within the CTGTCGGCTG sequence. One functional xenobiotic response element (XRE) located at the - 104/- 82 region is inducable by benzo[a]pyrene treatment. We believe our results have value for the study of GNMT transcriptional regulation.

Original languageEnglish
Pages (from-to)151-157
Number of pages7
JournalGene
Volume443
Issue number1-2
DOIs
Publication statusPublished - Aug 15 2009
Externally publishedYes

Fingerprint

Glycine N-Methyltransferase
Nucleic Acid Regulatory Sequences
Genes
Benzo(a)pyrene
Response Elements
Electrophoretic Mobility Shift Assay
Xenobiotics
Liver Neoplasms
Codon
Hepatocellular Carcinoma
Prostatic Neoplasms
Transcription Factors
Binding Sites
human GNMT protein
Mutation

Keywords

  • CCAAT box
  • GNMT
  • NF-Y
  • Promoter
  • Transcriptional start site

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene. / Lee, Cheng Ming; Shih, Yi Ping; Wu, Cho Han; Chen, Yi Ming Arthur.

In: Gene, Vol. 443, No. 1-2, 15.08.2009, p. 151-157.

Research output: Contribution to journalArticle

Lee, Cheng Ming ; Shih, Yi Ping ; Wu, Cho Han ; Chen, Yi Ming Arthur. / Characterization of the 5′ regulatory region of the human Glycine N-methyltransferase gene. In: Gene. 2009 ; Vol. 443, No. 1-2. pp. 151-157.
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