Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene

Chih Ming Chou, Chang Jen Huang, Chu Fang Lo, Guang Hsung Kou, Chung Hsiung Wang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV poyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin.

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalJournal of Invertebrate Pathology
Volume67
Issue number3
DOIs
Publication statusPublished - May 1996
Externally publishedYes

Keywords

  • Baculovirus
  • Perina nuda nucleopolyhedrovirus
  • Polyhedrin gene

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

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