Characterization of lens cyrstallins and their mRNA from the carp lenses

Shyh Horng Chiou, Tschining Chang, Wen Chang Chang, Jane Kuo, Tung Bin Lo

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Crystallins from carp eye lenses have been isolated and characterized by gel permeation chromatography, SDS-gel electrophoresis, immunodiffusion and amino acid analysis. γ-Crystallin is the most abundant class of crystallins and constitutes over 55% of the total lens cytoplasmic proteins. It is immunologically distinct from the α- and β-crystallins isolated from the same lens and its antiserum shows a very weak cross-reaction to total pig lens antigens. Comparison of the amino acid compositions of carp γ-crystallin with those of bovine γ-II, haddock γ- and squid crystallins indicates that γ-crystallin from the carp is very closely related to that of the haddock, and probably also related to the invertebrate squid cytstalline. In vitro translation of total mRNAs isolated from carp lenses confirms the predominant existence of γ-crystallin. The genomic characterization of carp crystallin genes should provide some insight into the mechanism of crystallin evolution in general.

Original languageEnglish
Pages (from-to)324-328
Number of pages5
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume871
Issue number3
DOIs
Publication statusPublished - Jun 23 1986
Externally publishedYes

Fingerprint

Crystallins
Carps
Lenses
Messenger RNA
Decapodiformes
Amino Acids
Crystalline Lens
Gel permeation chromatography
Immunodiffusion
Cross Reactions
Electrophoresis
Protein Biosynthesis
Invertebrates
Immune Sera
Gel Chromatography
Genes
Gels
Swine
Antigens

Keywords

  • (Fish, Pig, Squid)
  • Amino acid composition
  • Biochemical comparison
  • Immunodiffusion
  • Lens crystallin
  • Protein evolution

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Characterization of lens cyrstallins and their mRNA from the carp lenses. / Chiou, Shyh Horng; Chang, Tschining; Chang, Wen Chang; Kuo, Jane; Lo, Tung Bin.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 871, No. 3, 23.06.1986, p. 324-328.

Research output: Contribution to journalArticle

Chiou, Shyh Horng ; Chang, Tschining ; Chang, Wen Chang ; Kuo, Jane ; Lo, Tung Bin. / Characterization of lens cyrstallins and their mRNA from the carp lenses. In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1986 ; Vol. 871, No. 3. pp. 324-328.
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