Characterization of human growth hormone from acromegalic pituitary tumors

WEN‐CHANG ‐C CHANG, ALBERT LY‐YOUNG SHEN, CHEN‐KUNG ‐K CHOU, LOW‐TONE ‐T HO

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Human growth hormone (HGH) was extracted from acromegalic pituitary tumors at pH 10.5 and precipitated with ammonium sulfate at 20–40% saturation. It was purified on a Sephadex G‐100 column to yield monomeric HGH. The tumor‐HGH was indistinguishable from the authentic one in polyacrylamide gel electrophoresis at pH 8.3 or in the presence of sodium dodecyl sulfate, high‐performance liquid chromatography, radioimmunoassay, peptide map, amino acid composition and N‐terminal partial amino acid sequence. The tumor‐HGH is active in the tibia assay and body weight gain test in hypophysectomized rats with comparable potency to that of the authentic sample.

Original languageEnglish
Pages (from-to)637-641
Number of pages5
JournalInternational Journal of Peptide and Protein Research
Volume23
Issue number6
DOIs
Publication statusPublished - Jan 1 1984
Externally publishedYes

Fingerprint

Human Growth Hormone
Pituitary Neoplasms
Tumors
Amino Acids
Liquid chromatography
Ammonium Sulfate
Electrophoresis
Tibia
Liquid Chromatography
Sodium Dodecyl Sulfate
Weight Gain
Radioimmunoassay
Rats
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Assays
Body Weight
Peptides
Chemical analysis
sephadex

Keywords

  • human growth hormone
  • partial amino acid sequence
  • peptide mapping
  • pituitary tumors

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of human growth hormone from acromegalic pituitary tumors. / CHANG, WEN‐CHANG ‐C; SHEN, ALBERT LY‐YOUNG; CHOU, CHEN‐KUNG ‐K; HO, LOW‐TONE ‐T.

In: International Journal of Peptide and Protein Research, Vol. 23, No. 6, 01.01.1984, p. 637-641.

Research output: Contribution to journalArticle

CHANG, WEN‐CHANG ‐C ; SHEN, ALBERT LY‐YOUNG ; CHOU, CHEN‐KUNG ‐K ; HO, LOW‐TONE ‐T. / Characterization of human growth hormone from acromegalic pituitary tumors. In: International Journal of Peptide and Protein Research. 1984 ; Vol. 23, No. 6. pp. 637-641.
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