TY - JOUR
T1 - Characterization of human growth hormone from acromegalic pituitary tumors
AU - CHANG, WEN‐CHANG ‐C
AU - SHEN, ALBERT LY‐YOUNG
AU - CHOU, CHEN‐KUNG ‐K
AU - HO, LOW‐TONE ‐T
PY - 1984/1/1
Y1 - 1984/1/1
N2 - Human growth hormone (HGH) was extracted from acromegalic pituitary tumors at pH 10.5 and precipitated with ammonium sulfate at 20–40% saturation. It was purified on a Sephadex G‐100 column to yield monomeric HGH. The tumor‐HGH was indistinguishable from the authentic one in polyacrylamide gel electrophoresis at pH 8.3 or in the presence of sodium dodecyl sulfate, high‐performance liquid chromatography, radioimmunoassay, peptide map, amino acid composition and N‐terminal partial amino acid sequence. The tumor‐HGH is active in the tibia assay and body weight gain test in hypophysectomized rats with comparable potency to that of the authentic sample.
AB - Human growth hormone (HGH) was extracted from acromegalic pituitary tumors at pH 10.5 and precipitated with ammonium sulfate at 20–40% saturation. It was purified on a Sephadex G‐100 column to yield monomeric HGH. The tumor‐HGH was indistinguishable from the authentic one in polyacrylamide gel electrophoresis at pH 8.3 or in the presence of sodium dodecyl sulfate, high‐performance liquid chromatography, radioimmunoassay, peptide map, amino acid composition and N‐terminal partial amino acid sequence. The tumor‐HGH is active in the tibia assay and body weight gain test in hypophysectomized rats with comparable potency to that of the authentic sample.
KW - human growth hormone
KW - partial amino acid sequence
KW - peptide mapping
KW - pituitary tumors
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U2 - 10.1111/j.1399-3011.1984.tb03136.x
DO - 10.1111/j.1399-3011.1984.tb03136.x
M3 - Article
C2 - 6469461
AN - SCOPUS:0021227913
VL - 23
SP - 637
EP - 641
JO - International journal of protein research
JF - International journal of protein research
SN - 0367-8377
IS - 6
ER -