Characterization of human growth hormone from acromegalic pituitary tumors

WEN‐CHANG ‐C CHANG; ALBERT LY‐YOUNG SHEN; CHEN‐KUNG ‐K CHOU; LOW‐TONE ‐T HO

doi:10.1111/j.1399-3011.1984.tb03136.x

Characterization of human growth hormone from acromegalic pituitary tumors

WEN‐CHANG ‐C CHANG, ALBERT LY‐YOUNG SHEN, CHEN‐KUNG ‐K CHOU, LOW‐TONE ‐T HO

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Human growth hormone (HGH) was extracted from acromegalic pituitary tumors at pH 10.5 and precipitated with ammonium sulfate at 20–40% saturation. It was purified on a Sephadex G‐100 column to yield monomeric HGH. The tumor‐HGH was indistinguishable from the authentic one in polyacrylamide gel electrophoresis at pH 8.3 or in the presence of sodium dodecyl sulfate, high‐performance liquid chromatography, radioimmunoassay, peptide map, amino acid composition and N‐terminal partial amino acid sequence. The tumor‐HGH is active in the tibia assay and body weight gain test in hypophysectomized rats with comparable potency to that of the authentic sample.

Original language	English
Pages (from-to)	637-641
Number of pages	5
Journal	International Journal of Peptide and Protein Research
Volume	23
Issue number	6
DOIs	https://doi.org/10.1111/j.1399-3011.1984.tb03136.x
Publication status	Published - Jan 1 1984
Externally published	Yes

Keywords

human growth hormone
partial amino acid sequence
peptide mapping
pituitary tumors

ASJC Scopus subject areas

Biochemistry

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abstract = "Human growth hormone (HGH) was extracted from acromegalic pituitary tumors at pH 10.5 and precipitated with ammonium sulfate at 20–40% saturation. It was purified on a Sephadex G‐100 column to yield monomeric HGH. The tumor‐HGH was indistinguishable from the authentic one in polyacrylamide gel electrophoresis at pH 8.3 or in the presence of sodium dodecyl sulfate, high‐performance liquid chromatography, radioimmunoassay, peptide map, amino acid composition and N‐terminal partial amino acid sequence. The tumor‐HGH is active in the tibia assay and body weight gain test in hypophysectomized rats with comparable potency to that of the authentic sample.",

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AU - CHANG, WEN‐CHANG ‐C

AU - SHEN, ALBERT LY‐YOUNG

AU - CHOU, CHEN‐KUNG ‐K

AU - HO, LOW‐TONE ‐T

PY - 1984/1/1

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