Characterization of glycine-N-methyltransferase-gene expression in human hepatocellular carcinoma

Yi Ming A. Chen, Jer Yuan A. Shiu, Shwu Jen Tzeng, Li Sun Shih, Yi Jen Chen, Wing Yiu Lut, Pao Huei Chen

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Messenger RNA differential display was used to study liver-gene expression in paired tumor and non-tumor tissues from hepatocellular carcinoma (HCC) patients. mRNA differential display and Northern-blot analyses showed that a 0.8-kb cDNA fragment was diminished or absent from the tumorous tissues of 7 HCC patients. The cDNA fragment was sequenced and found to have 98.7% nucleotide sequence homology with human glycine-N- methyltransferase cDNA (GNMT). In addition, there was no detectable level of GNMT expression in 4 human HCC cell lines, SK-Hep1, Hep 3B, HuH-7 and HA22T, examined by Northern-blot assay. A full-length GNMT cDNA clone-9-1-2 was obtained by screening a Taiwanese liver cDNA library. In comparison with the GNMT cDNA sequence reported elsewhere, clone 9-1-2 had 4 nucleotide differences resulting in 1 amino-acid change. Immunohistochemical staining with rabbit anti-recombinant GNMT serum showed that GNMT protein almost completely disappeared in liver-cancer cells, while it was abundant in the non-tumorous liver cells. Down-regulation of GNMT gene expression may be involved in the pathogenesis of liver cancer.

Original languageEnglish
Pages (from-to)787-793
Number of pages7
JournalInternational Journal of Cancer
Volume75
Issue number5
DOIs
Publication statusPublished - Mar 2 1998
Externally publishedYes

Fingerprint

Glycine N-Methyltransferase
Hepatocellular Carcinoma
Complementary DNA
Gene Expression
Liver Neoplasms
Northern Blotting
Liver
Clone Cells
Gene Expression Profiling
Sequence Homology
Gene Library

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Characterization of glycine-N-methyltransferase-gene expression in human hepatocellular carcinoma. / Chen, Yi Ming A.; Shiu, Jer Yuan A.; Tzeng, Shwu Jen; Shih, Li Sun; Chen, Yi Jen; Lut, Wing Yiu; Chen, Pao Huei.

In: International Journal of Cancer, Vol. 75, No. 5, 02.03.1998, p. 787-793.

Research output: Contribution to journalArticle

Chen, Yi Ming A. ; Shiu, Jer Yuan A. ; Tzeng, Shwu Jen ; Shih, Li Sun ; Chen, Yi Jen ; Lut, Wing Yiu ; Chen, Pao Huei. / Characterization of glycine-N-methyltransferase-gene expression in human hepatocellular carcinoma. In: International Journal of Cancer. 1998 ; Vol. 75, No. 5. pp. 787-793.
@article{28ee1fb4d93d441ab7ec8c4707f97a50,
title = "Characterization of glycine-N-methyltransferase-gene expression in human hepatocellular carcinoma",
abstract = "Messenger RNA differential display was used to study liver-gene expression in paired tumor and non-tumor tissues from hepatocellular carcinoma (HCC) patients. mRNA differential display and Northern-blot analyses showed that a 0.8-kb cDNA fragment was diminished or absent from the tumorous tissues of 7 HCC patients. The cDNA fragment was sequenced and found to have 98.7{\%} nucleotide sequence homology with human glycine-N- methyltransferase cDNA (GNMT). In addition, there was no detectable level of GNMT expression in 4 human HCC cell lines, SK-Hep1, Hep 3B, HuH-7 and HA22T, examined by Northern-blot assay. A full-length GNMT cDNA clone-9-1-2 was obtained by screening a Taiwanese liver cDNA library. In comparison with the GNMT cDNA sequence reported elsewhere, clone 9-1-2 had 4 nucleotide differences resulting in 1 amino-acid change. Immunohistochemical staining with rabbit anti-recombinant GNMT serum showed that GNMT protein almost completely disappeared in liver-cancer cells, while it was abundant in the non-tumorous liver cells. Down-regulation of GNMT gene expression may be involved in the pathogenesis of liver cancer.",
author = "Chen, {Yi Ming A.} and Shiu, {Jer Yuan A.} and Tzeng, {Shwu Jen} and Shih, {Li Sun} and Chen, {Yi Jen} and Lut, {Wing Yiu} and Chen, {Pao Huei}",
year = "1998",
month = "3",
day = "2",
doi = "10.1002/(SICI)1097-0215(19980302)75:5<787::AID-IJC20>3.0.CO;2-2",
language = "English",
volume = "75",
pages = "787--793",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - Characterization of glycine-N-methyltransferase-gene expression in human hepatocellular carcinoma

AU - Chen, Yi Ming A.

AU - Shiu, Jer Yuan A.

AU - Tzeng, Shwu Jen

AU - Shih, Li Sun

AU - Chen, Yi Jen

AU - Lut, Wing Yiu

AU - Chen, Pao Huei

PY - 1998/3/2

Y1 - 1998/3/2

N2 - Messenger RNA differential display was used to study liver-gene expression in paired tumor and non-tumor tissues from hepatocellular carcinoma (HCC) patients. mRNA differential display and Northern-blot analyses showed that a 0.8-kb cDNA fragment was diminished or absent from the tumorous tissues of 7 HCC patients. The cDNA fragment was sequenced and found to have 98.7% nucleotide sequence homology with human glycine-N- methyltransferase cDNA (GNMT). In addition, there was no detectable level of GNMT expression in 4 human HCC cell lines, SK-Hep1, Hep 3B, HuH-7 and HA22T, examined by Northern-blot assay. A full-length GNMT cDNA clone-9-1-2 was obtained by screening a Taiwanese liver cDNA library. In comparison with the GNMT cDNA sequence reported elsewhere, clone 9-1-2 had 4 nucleotide differences resulting in 1 amino-acid change. Immunohistochemical staining with rabbit anti-recombinant GNMT serum showed that GNMT protein almost completely disappeared in liver-cancer cells, while it was abundant in the non-tumorous liver cells. Down-regulation of GNMT gene expression may be involved in the pathogenesis of liver cancer.

AB - Messenger RNA differential display was used to study liver-gene expression in paired tumor and non-tumor tissues from hepatocellular carcinoma (HCC) patients. mRNA differential display and Northern-blot analyses showed that a 0.8-kb cDNA fragment was diminished or absent from the tumorous tissues of 7 HCC patients. The cDNA fragment was sequenced and found to have 98.7% nucleotide sequence homology with human glycine-N- methyltransferase cDNA (GNMT). In addition, there was no detectable level of GNMT expression in 4 human HCC cell lines, SK-Hep1, Hep 3B, HuH-7 and HA22T, examined by Northern-blot assay. A full-length GNMT cDNA clone-9-1-2 was obtained by screening a Taiwanese liver cDNA library. In comparison with the GNMT cDNA sequence reported elsewhere, clone 9-1-2 had 4 nucleotide differences resulting in 1 amino-acid change. Immunohistochemical staining with rabbit anti-recombinant GNMT serum showed that GNMT protein almost completely disappeared in liver-cancer cells, while it was abundant in the non-tumorous liver cells. Down-regulation of GNMT gene expression may be involved in the pathogenesis of liver cancer.

UR - http://www.scopus.com/inward/record.url?scp=0032473408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032473408&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-0215(19980302)75:5<787::AID-IJC20>3.0.CO;2-2

DO - 10.1002/(SICI)1097-0215(19980302)75:5<787::AID-IJC20>3.0.CO;2-2

M3 - Article

VL - 75

SP - 787

EP - 793

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 5

ER -