Characterization of dna complementary to nucleotide sequences adjacent to poly(A) at the 3′-terminus of the avian sarcoma virus genome

Jacov Tal, Hsing Jien Kung, Harold E. Varmus, J. Michael Bishop

Research output: Contribution to journalArticle

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Abstract

We have prepared DNA complementary to a sequence of 300-400 nucleotides adjacent to the poly(A) at the 3′-terminus of the genome of avian sarcoma virus (ASV) DNA was transcribed from denatured ASV RNA following initiation on the primer (dT)12-18. We demonstrated that homopolymer synthesis was not a significant feature of our enzymatic reaction, and we proved that most if not all the initiations on (dT)12-18 occurred at or near the 3'-terminus of the viral RNA. The presence of oligo(dT) on cDNA3, accelerates hybridization between cDNA3, and polyadenylated viral RNA; this artifact can be eliminated by including a competing homopolymer [poly(dT) or poly(A)l in the reaction mixtures. Nucleotide sequences complementary to cDNA3, occur only once or twice in the genome of avian sarcoma viruses, are present in the genomes of virus strains from subgroups A, B, C, D, E, and F of avian leukosis-sarcoma viruses, and are conserved even when large deletions affect an adjacent viral gene (src) By contrast, we find little or no complementarity between cDNA3, and the genomes of golden pheasant virus (subgroup G of avian leukosis-sarcoma viruses), mouse mammary tumor virus, and the Moloney strains of murine sarcoma virus and murine leukemia virus. cDNA3, can be used to identify the 3′-terminus of the avian sarcoma virus genome by molecular hybridization and will therefore be a useful reagent for the analysis of viral replication.

Original languageEnglish
Pages (from-to)183-197
Number of pages15
JournalVirology
Volume79
Issue number1
DOIs
Publication statusPublished - Jun 1 1977
Externally publishedYes

Fingerprint

Avian Sarcoma Viruses
Poly A
Genome
Alpharetrovirus
Viral RNA
Moloney murine sarcoma virus
Poly T
Viruses
Mouse mammary tumor virus
Murine Leukemia Viruses
Viral Genes
Artifacts
Nucleotides
Complementary DNA
RNA
Messenger RNA
DNA

ASJC Scopus subject areas

  • Virology

Cite this

Characterization of dna complementary to nucleotide sequences adjacent to poly(A) at the 3′-terminus of the avian sarcoma virus genome. / Tal, Jacov; Kung, Hsing Jien; Varmus, Harold E.; Bishop, J. Michael.

In: Virology, Vol. 79, No. 1, 01.06.1977, p. 183-197.

Research output: Contribution to journalArticle

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