Characterization of chitosan-gelatin scaffolds for dermal tissue engineering

Hsiang Jung Tseng, Tai Li Tsou, Hsian Jenn Wang, Shan hui Hsu

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Porous scaffolds for dermal tissue engineering were fabricated by freeze-drying a mixture of chitosan and gelatin (CG) solutions. Different crosslinking agents including glutaraldehyde, 1-(3-dimethylaminopropyl)-3-ethyl-carbodimide hydrochloride (EDC), and genipin were used to crosslink the scaffolds and improve their biostability. The porous structure and mechanical properties were determined for the scaffolds. The proliferation of human fibroblasts in the scaffolds was analyzed. It was found that EDC crosslinked scaffolds had the greatest amount of cells after four days. EDC crosslinked CG scaffolds had tensile modulus in a dry state and compressive modulus in a wet state similar to commercial collagen wound dressing. They also showed appropriate pore size, high water absorption, and good dimensional stability during cell culture. When human fibroblasts were seeded on acellular porcine dermis (APD), acellular human dermis (AHD), and CG scaffolds for 3D cell culture, they were well-distributed in the centre of the CG scaffolds but stayed only on the superficial layer of APD or AHD after seven days. A gelatin-based bioglue was applied to the CG scaffolds where the keratinocytes were seeded to mimic epidermal structure. After 14days, the bioglue degraded and keratinocytes grew to form monolayers on the scaffolds. This study showed that CG scaffolds crosslinked by EDC and seeded with human fibroblasts could serve as dermal constructs, while the bioglue coating seeded with keratinocytes could serve as an epidermal construct. Such a combination could help regenerate skin with integrated dermal and epidermal layers and a have potential use in tissue-engineered skin.

Original languageEnglish
Pages (from-to)20-31
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Chitosan
Gelatin
Tissue Engineering
Scaffolds (biology)
Tissue engineering
Scaffolds
Acellular Dermis
Skin
Keratinocytes
Fibroblasts
Swine
Cell Culture Techniques
Cell culture
Freeze Drying
Glutaral
Bandages
Forms (concrete)
Dimensional stability
Water absorption
Collagen

Keywords

  • Artificial skin
  • Bioglue
  • Chitosan
  • Dermis
  • Gelatin
  • Scaffold

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Characterization of chitosan-gelatin scaffolds for dermal tissue engineering. / Tseng, Hsiang Jung; Tsou, Tai Li; Wang, Hsian Jenn; Hsu, Shan hui.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 7, No. 1, 01.2013, p. 20-31.

Research output: Contribution to journalArticle

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