Characterization of and mechanism for copper-induced thioureation of serum albumin

Yu Wei Wu, Yu Hui Tsai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Thioureas (Tus) are widely used in chemical and pharmaceutical industries. This study demonstrated that copper induced the disulfide-linkage between Tus, such as α-naphthylthiourea (ANTU) and fluorescein-5-isothiocyanate cadaverine (FTC), with albumin (Alb), a major carrier protein in plasma with multiple functions. This reaction was absolutely copper-dependent, whereas cobalt, nickel, calcium, magnesium, zinc, iron, and manganese ions could not induce the same reaction. The reaction was substrate dose-dependent, and occurred optimally at pH 6.5. The resulting conjugated product was heat-labile, but stable in pH 6.0-8.0 buffer at 25°C. The linkage could be reduced by Cu(I) (in acidic pH) and thiol-reducing agents. The mechanism of albumin thioureation was concluded: (i) the binding of Cu(II) with albumin is not necessary for the reaction, while the formation of Tus-Cu(II) complex is essential; (ii) thioureation resulted from the attack of Tus-Cu(II) at Alb-Cys34-SH to form the Alb-Cys34-S-S-Tus complex accompanied by the release of Cu(I); (iii) the released Cu(I) would back inhibit the reaction because of its competition with Cu(II) for Tus binding. These phenomenons may have important implications for the pharmacokinetics of thiourea-based drugs in plasma.

Original languageEnglish
Pages (from-to)1822-1830
Number of pages9
JournalBioconjugate Chemistry
Volume19
Issue number9
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Thiourea
Thioureas
Serum Albumin
Copper
Albumins
Cadaverine
Plasmas
Chemical Industry
Pharmacokinetics
Fluorescein-5-isothiocyanate
Reducing Agents
Drug Industry
Reducing agents
Manganese
Cobalt
Nickel
Sulfhydryl Compounds
Disulfides
Pharmaceutical Preparations
Drug products

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Characterization of and mechanism for copper-induced thioureation of serum albumin. / Wu, Yu Wei; Tsai, Yu Hui.

In: Bioconjugate Chemistry, Vol. 19, No. 9, 09.2008, p. 1822-1830.

Research output: Contribution to journalArticle

Wu, Yu Wei ; Tsai, Yu Hui. / Characterization of and mechanism for copper-induced thioureation of serum albumin. In: Bioconjugate Chemistry. 2008 ; Vol. 19, No. 9. pp. 1822-1830.
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