Characterization of a Suppressor-Cell Leukemia: Evidence for the Requirement of an Interaction of Two T Cells in the Development of Human Suppressor Effector Cells

Samuel Broder, David Poplack, Jacqueline Whang-Peng, Mary Durm, Carolyn Goldman, Linda Muul, Thomas A. Waldmann

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

To characterize the suppressor activity of neoplastic T cells from a child with acute lymphoblastic leukemia and hypogammaglobulinemia, we applied an in vitro assay that determines the capacity of pokeweed-mitogen-stimulated lymphocytes to mature into immunoglobulin-secreting cells. The geometric mean synthesis by peripheral blood lymphocytes from 12 normal persons was 3200 ng for IgM, 2447 ng for IgG and 1825 ng for IgA (2 X 106 cells per 12 days in culture). The patient's leukemic cells produced no detectable immunoglobulin and depressed the immunoglobulin production of normal lymphocytes by 85 to 100 per cent in co-culture experiments. However, suppression was observed only when co-operating normal T cells were present. Prior irradiation of either the leukemic T cells or the co-operating normal T cells nullified the suppressor effect. Therefore, an interaction between at least two different T-cell subsets may be required for the generation of suppressor effector T cells in man. (N Engl J Med 298:66–72, 1978) NORMAL humoral immunity is based on an intricate network of co-operating lymphocytes and accessory cells. Thymic-derived lymphocytes (T cells) play an important part in the regulation of humoral immune responses by acting as potentiators or inhibitors of the transition of bone-marrow-derived lymphocytes (B cells) into immunoglobulin-secreting plasma cells. The T cells that potentiate this B-cell transition are categorized as helper cells, whereas those that inhibit the transition are categorized as suppressor cells.1 2 3 Help and suppression are mediated by different populations of T cells, each genetically committed to mediate only one of these two functions.4 5 Neoplasms of T-cell origin are of.

Original languageEnglish
Pages (from-to)66-72
Number of pages7
JournalNew England Journal of Medicine
Volume298
Issue number2
DOIs
Publication statusPublished - Jan 12 1978
Externally publishedYes

Fingerprint

Human Development
Leukemia
T-Lymphocytes
Lymphocytes
Antibody-Producing Cells
Humoral Immunity
Immunoglobulins
B-Lymphocytes
Agammaglobulinemia
Pokeweed Mitogens
T-Lymphocyte Subsets
Helper-Inducer T-Lymphocytes
Coculture Techniques
Plasma Cells
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Immunoglobulin A
Immunoglobulin M
Immunoglobulin G
Bone Marrow

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Characterization of a Suppressor-Cell Leukemia : Evidence for the Requirement of an Interaction of Two T Cells in the Development of Human Suppressor Effector Cells. / Broder, Samuel; Poplack, David; Whang-Peng, Jacqueline; Durm, Mary; Goldman, Carolyn; Muul, Linda; Waldmann, Thomas A.

In: New England Journal of Medicine, Vol. 298, No. 2, 12.01.1978, p. 66-72.

Research output: Contribution to journalArticle

@article{923aca881aae4fbf9be63b2096b1b85e,
title = "Characterization of a Suppressor-Cell Leukemia: Evidence for the Requirement of an Interaction of Two T Cells in the Development of Human Suppressor Effector Cells",
abstract = "To characterize the suppressor activity of neoplastic T cells from a child with acute lymphoblastic leukemia and hypogammaglobulinemia, we applied an in vitro assay that determines the capacity of pokeweed-mitogen-stimulated lymphocytes to mature into immunoglobulin-secreting cells. The geometric mean synthesis by peripheral blood lymphocytes from 12 normal persons was 3200 ng for IgM, 2447 ng for IgG and 1825 ng for IgA (2 X 106 cells per 12 days in culture). The patient's leukemic cells produced no detectable immunoglobulin and depressed the immunoglobulin production of normal lymphocytes by 85 to 100 per cent in co-culture experiments. However, suppression was observed only when co-operating normal T cells were present. Prior irradiation of either the leukemic T cells or the co-operating normal T cells nullified the suppressor effect. Therefore, an interaction between at least two different T-cell subsets may be required for the generation of suppressor effector T cells in man. (N Engl J Med 298:66–72, 1978) NORMAL humoral immunity is based on an intricate network of co-operating lymphocytes and accessory cells. Thymic-derived lymphocytes (T cells) play an important part in the regulation of humoral immune responses by acting as potentiators or inhibitors of the transition of bone-marrow-derived lymphocytes (B cells) into immunoglobulin-secreting plasma cells. The T cells that potentiate this B-cell transition are categorized as helper cells, whereas those that inhibit the transition are categorized as suppressor cells.1 2 3 Help and suppression are mediated by different populations of T cells, each genetically committed to mediate only one of these two functions.4 5 Neoplasms of T-cell origin are of.",
author = "Samuel Broder and David Poplack and Jacqueline Whang-Peng and Mary Durm and Carolyn Goldman and Linda Muul and Waldmann, {Thomas A.}",
year = "1978",
month = "1",
day = "12",
doi = "10.1056/NEJM197801122980202",
language = "English",
volume = "298",
pages = "66--72",
journal = "New England Journal of Medicine",
issn = "0028-4793",
publisher = "Massachussetts Medical Society",
number = "2",

}

TY - JOUR

T1 - Characterization of a Suppressor-Cell Leukemia

T2 - Evidence for the Requirement of an Interaction of Two T Cells in the Development of Human Suppressor Effector Cells

AU - Broder, Samuel

AU - Poplack, David

AU - Whang-Peng, Jacqueline

AU - Durm, Mary

AU - Goldman, Carolyn

AU - Muul, Linda

AU - Waldmann, Thomas A.

PY - 1978/1/12

Y1 - 1978/1/12

N2 - To characterize the suppressor activity of neoplastic T cells from a child with acute lymphoblastic leukemia and hypogammaglobulinemia, we applied an in vitro assay that determines the capacity of pokeweed-mitogen-stimulated lymphocytes to mature into immunoglobulin-secreting cells. The geometric mean synthesis by peripheral blood lymphocytes from 12 normal persons was 3200 ng for IgM, 2447 ng for IgG and 1825 ng for IgA (2 X 106 cells per 12 days in culture). The patient's leukemic cells produced no detectable immunoglobulin and depressed the immunoglobulin production of normal lymphocytes by 85 to 100 per cent in co-culture experiments. However, suppression was observed only when co-operating normal T cells were present. Prior irradiation of either the leukemic T cells or the co-operating normal T cells nullified the suppressor effect. Therefore, an interaction between at least two different T-cell subsets may be required for the generation of suppressor effector T cells in man. (N Engl J Med 298:66–72, 1978) NORMAL humoral immunity is based on an intricate network of co-operating lymphocytes and accessory cells. Thymic-derived lymphocytes (T cells) play an important part in the regulation of humoral immune responses by acting as potentiators or inhibitors of the transition of bone-marrow-derived lymphocytes (B cells) into immunoglobulin-secreting plasma cells. The T cells that potentiate this B-cell transition are categorized as helper cells, whereas those that inhibit the transition are categorized as suppressor cells.1 2 3 Help and suppression are mediated by different populations of T cells, each genetically committed to mediate only one of these two functions.4 5 Neoplasms of T-cell origin are of.

AB - To characterize the suppressor activity of neoplastic T cells from a child with acute lymphoblastic leukemia and hypogammaglobulinemia, we applied an in vitro assay that determines the capacity of pokeweed-mitogen-stimulated lymphocytes to mature into immunoglobulin-secreting cells. The geometric mean synthesis by peripheral blood lymphocytes from 12 normal persons was 3200 ng for IgM, 2447 ng for IgG and 1825 ng for IgA (2 X 106 cells per 12 days in culture). The patient's leukemic cells produced no detectable immunoglobulin and depressed the immunoglobulin production of normal lymphocytes by 85 to 100 per cent in co-culture experiments. However, suppression was observed only when co-operating normal T cells were present. Prior irradiation of either the leukemic T cells or the co-operating normal T cells nullified the suppressor effect. Therefore, an interaction between at least two different T-cell subsets may be required for the generation of suppressor effector T cells in man. (N Engl J Med 298:66–72, 1978) NORMAL humoral immunity is based on an intricate network of co-operating lymphocytes and accessory cells. Thymic-derived lymphocytes (T cells) play an important part in the regulation of humoral immune responses by acting as potentiators or inhibitors of the transition of bone-marrow-derived lymphocytes (B cells) into immunoglobulin-secreting plasma cells. The T cells that potentiate this B-cell transition are categorized as helper cells, whereas those that inhibit the transition are categorized as suppressor cells.1 2 3 Help and suppression are mediated by different populations of T cells, each genetically committed to mediate only one of these two functions.4 5 Neoplasms of T-cell origin are of.

UR - http://www.scopus.com/inward/record.url?scp=0017805501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017805501&partnerID=8YFLogxK

U2 - 10.1056/NEJM197801122980202

DO - 10.1056/NEJM197801122980202

M3 - Article

C2 - 304175

AN - SCOPUS:0017805501

VL - 298

SP - 66

EP - 72

JO - New England Journal of Medicine

JF - New England Journal of Medicine

SN - 0028-4793

IS - 2

ER -