Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts

Tsu Yi Chao, T. Ming Chu

Research output: Contribution to journalArticle

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Abstract

A mouse mammary tumor cell line, desingated JC, has been established from a spontaneously developed primary adenocarcinoma of an aged virgin female BALB/c mouse. Isoenzyme analyses including glucose-6-phosphate dehydrogenase, lactate dehydrogenase, and peptidase proved that this cell line is of murine origin and devoid of contamination from other species. Karyotyping revealed that the number of chromosome ranged from 26 to 100, with a modal number of 40. Electron microscopic examination detected the presence of tonofilament and desmosomes confirming its epithelial nature. In addition, no type B or C virus particle was detected, although intracysternal A particle was observed occasionally. Tumorigenicity in immunocompetent syngeneic hosts was easily established by s.c., i.p., and i.v. injection of viable JC tumor cells. A very weak immunogenicity of the JC tumor was demonstrated through its immunization-challenging on syngeneic immunocompetent hosts. Although no rejection of JC tumor was noted, a significant prolongation for the incubation period before an obvious and palpable tumor growth was detected between the experimental and the control animals. Development of a concomitant immunity was also detected. The JC tumor represents a valuable murine mammary tumor model which is different from other available models because of its unique origin, absence of virus particles, very weak immunogenicity, and high tumorigenicity in syngeneic hosts. The cell line has been maintained for more than 5 yr and has been used for experimental immunotherapy in our laboratory.

Original languageEnglish
Pages (from-to)621-626
Number of pages6
JournalIn Vitro Cellular & Developmental Biology
Volume25
Issue number7
DOIs
Publication statusPublished - Jul 1989
Externally publishedYes

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Tumors
Adenocarcinoma
Breast
Cells
Neoplasms
Virion
Viruses
Breast Neoplasms
Cell Line
Karyotyping
Desmosomes
Intermediate Filaments
Glucosephosphate Dehydrogenase
Tumor Cell Line
L-Lactate Dehydrogenase
Immunization
Immunotherapy
Isoenzymes
Immunity
Peptide Hydrolases

Keywords

  • BALB/c mouse
  • immunogenicity
  • murine mammary tumor

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Clinical Biochemistry

Cite this

Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. / Chao, Tsu Yi; Chu, T. Ming.

In: In Vitro Cellular & Developmental Biology, Vol. 25, No. 7, 07.1989, p. 621-626.

Research output: Contribution to journalArticle

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