Characterization of a new plasmid-like prophage In a pandemic Vibrio parahaemolyticus O3:K6 strain

Shih Feng Lan, Chung Ho Huang, Chuan Hsiung Chang, Wei Chao Liao, I. Hsuan Lin, Wan Neng Jian, Yueh Gin Wu, Shau Yan Chen, Hin Chung Wong

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Vibrio parahaemolyticus is a common food-borne pathogen that is normally associated with seafood. In 1996, a pandemic O3:K6 strain abruptly appeared and caused the first pandemic of this pathogen to spread throughout many Asian countries, America, Europe, and Africa. The role of temperate bacteriophages in the evolution of this pathogen is of great interest. In this work, a new temperate phage, VP882, from a pandemic O3:K6 strain of V. parahaemolyticus was purified and characterized after mitomycin C induction. VP882 was a Myoviridae bacteriophage with a polyhedral head and a long rigid tail with a sheath-like structure. It infected and lysed high proportions of V. parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae strains. The genome of phage VP882 was sequenced and was 38,197 bp long, and 71 putative open reading frames were identified, of which 27 were putative functional phage or bacterial genes. VP882 had a linear plasmid-like genome with a putative protelomerase gene and cohesive ends. The genome does not integrate into the host chromosome but was maintained as a plasmid in the lysogen. Analysis of the reaction sites of the protelomerases in different plasmid-like phages revealed that VP882 and ΦHAP-1 were highly similar, while N15, ΦKO2, and PY54 made up another closely related group. The presence of DNA adenine methylase and quorum-sensing transcriptional regulators in VP882 may play a specific role in this phage or regulate physiological or virulence-associated traits of the hosts. These genes may also be remnants from the bacterial chromosome following transduction.

Original languageEnglish
Pages (from-to)2659-2667
Number of pages9
JournalApplied and Environmental Microbiology
Volume75
Issue number9
DOIs
Publication statusPublished - May 1 2009
Externally publishedYes

Fingerprint

Vibrio parahaemolyticus
Prophages
Pandemics
pandemic
plasmid
bacteriophages
Bacteriophages
plasmids
Plasmids
genome
pathogen
bacteriophage
gene
chromosome
cholera
seafood
virulence
Genome
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Myoviridae

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Characterization of a new plasmid-like prophage In a pandemic Vibrio parahaemolyticus O3:K6 strain. / Lan, Shih Feng; Huang, Chung Ho; Chang, Chuan Hsiung; Liao, Wei Chao; Lin, I. Hsuan; Jian, Wan Neng; Wu, Yueh Gin; Chen, Shau Yan; Wong, Hin Chung.

In: Applied and Environmental Microbiology, Vol. 75, No. 9, 01.05.2009, p. 2659-2667.

Research output: Contribution to journalArticle

Lan, SF, Huang, CH, Chang, CH, Liao, WC, Lin, IH, Jian, WN, Wu, YG, Chen, SY & Wong, HC 2009, 'Characterization of a new plasmid-like prophage In a pandemic Vibrio parahaemolyticus O3:K6 strain', Applied and Environmental Microbiology, vol. 75, no. 9, pp. 2659-2667. https://doi.org/10.1128/AEM.02483-08
Lan, Shih Feng ; Huang, Chung Ho ; Chang, Chuan Hsiung ; Liao, Wei Chao ; Lin, I. Hsuan ; Jian, Wan Neng ; Wu, Yueh Gin ; Chen, Shau Yan ; Wong, Hin Chung. / Characterization of a new plasmid-like prophage In a pandemic Vibrio parahaemolyticus O3:K6 strain. In: Applied and Environmental Microbiology. 2009 ; Vol. 75, No. 9. pp. 2659-2667.
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