Characterization of a Human Ovarian Carcinoma Cell Line (NIH

OVCAR-3) with Androgen and Estrogen Receptors

Thomas C. Hamilton, Robert C. Young, Wilma M. McKoy, Karen R. Grotzinger, John A. Green, Jacqueline Whang-Peng, Alfred M. Rogan, Robert F. Ozols, Elizabeth W. Chu, William R. Green

Research output: Contribution to journalArticle

443 Citations (Scopus)

Abstract

A cell line, NIH:OVCAR-3 has been established from the malignant ascites of a patient with progressive adenocarcinome of the ovary after combination chemotherapy with cyclophosphamide, Adriamycin, and cisplatin. OVCAR-3 grows as a cobble-stone-like monolayer with foci of multilayering, is tumorigenic in athymic mice, clones in agarose, and has an abnormal karyotype which includes a homogeneous staining region and a double minute chromosome. The cultured cells and xenografts contain cytoplasmic androgen and estrogen-binding macromolecules with the specificity of the respective steroid hormone receptors. These components have sedimentation coefficients of 7 to 9S in low-salt sucrose-density gradients, have dissociation constants of 250 and 9.6 pM, and are present at concentrations of 30 and 28 fmol/mg cytosol protein characteristic of androgen and estrogen receptors, respectively. OVCAR-3 is resistant in vitro to clinically relevant concentrations of Adriamycin (5 x 10-8M) melphalan (5 x 10-6M), and cisplatin (5 x 10–7M) with survival compared to untreated controls of 43,45, and 77%, respectively. Furthermore, there are multiple histological similarities between the patient's original tumor, the cell line, and the transplantable tumor. These data indicate that OVCAR-3 may be of use for investigations as to the significance of androgens and estrogens and the mechanisms of cytotoxic drug resistance in ovarian cancer.

Original languageEnglish
Pages (from-to)5379-5389
Number of pages11
JournalCancer Research
Volume43
Issue number11
Publication statusPublished - Nov 1 1983
Externally publishedYes

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Androgen Receptors
Estrogen Receptors
Doxorubicin
Cisplatin
Androgens
Estrogens
Abnormal Karyotype
Carcinoma
Cell Line
Melphalan
Steroid Receptors
Combination Drug Therapy
Tumor Cell Line
Ascites
Drug Resistance
Heterografts
Nude Mice
Cytosol
Ovarian Neoplasms
Sepharose

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Hamilton, T. C., Young, R. C., McKoy, W. M., Grotzinger, K. R., Green, J. A., Whang-Peng, J., ... Green, W. R. (1983). Characterization of a Human Ovarian Carcinoma Cell Line (NIH: OVCAR-3) with Androgen and Estrogen Receptors. Cancer Research, 43(11), 5379-5389.

Characterization of a Human Ovarian Carcinoma Cell Line (NIH : OVCAR-3) with Androgen and Estrogen Receptors. / Hamilton, Thomas C.; Young, Robert C.; McKoy, Wilma M.; Grotzinger, Karen R.; Green, John A.; Whang-Peng, Jacqueline; Rogan, Alfred M.; Ozols, Robert F.; Chu, Elizabeth W.; Green, William R.

In: Cancer Research, Vol. 43, No. 11, 01.11.1983, p. 5379-5389.

Research output: Contribution to journalArticle

Hamilton, TC, Young, RC, McKoy, WM, Grotzinger, KR, Green, JA, Whang-Peng, J, Rogan, AM, Ozols, RF, Chu, EW & Green, WR 1983, 'Characterization of a Human Ovarian Carcinoma Cell Line (NIH: OVCAR-3) with Androgen and Estrogen Receptors', Cancer Research, vol. 43, no. 11, pp. 5379-5389.
Hamilton TC, Young RC, McKoy WM, Grotzinger KR, Green JA, Whang-Peng J et al. Characterization of a Human Ovarian Carcinoma Cell Line (NIH: OVCAR-3) with Androgen and Estrogen Receptors. Cancer Research. 1983 Nov 1;43(11):5379-5389.
Hamilton, Thomas C. ; Young, Robert C. ; McKoy, Wilma M. ; Grotzinger, Karen R. ; Green, John A. ; Whang-Peng, Jacqueline ; Rogan, Alfred M. ; Ozols, Robert F. ; Chu, Elizabeth W. ; Green, William R. / Characterization of a Human Ovarian Carcinoma Cell Line (NIH : OVCAR-3) with Androgen and Estrogen Receptors. In: Cancer Research. 1983 ; Vol. 43, No. 11. pp. 5379-5389.
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