Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker

Yung Chieh Tasi, Hsin Chih Albert Chao, Chia Ling Chung, Xiu Ying Liu, Ying Ming Lin, Pao Chi Liao, Hsien An Pan, Han-Sun Chiang, Pao Lin Kuo, Ying Hung Lin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: Asthenozoospermia is a major cause of male infertility. However, the molecular mechanisms underlying sperm-motility defects remain largely unknown in the majority of cases. In our previous study, we applied a proteomic approach to identify unknown proteins that were downregulated in spermatozoa with low motility compared to spermatozoa with good motility. Several sperm motility- related proteins have been identified. In this study, 3-hydroxyisobutyrate dehydrogenase (HIBADH), one of the proteins identified using the proteomic tools, is further characterized. Methods: Reverse-transcription polymerase chain reactions (RT-PCR), western blotting, and immunofluorescence assays (IFA) were preformed to investigate the expression pattern. The enzymatic activity of HIBADH was evaluated in sperm with good (>50 %), moderate (<50 %) and lower motility (<20 %). Results: Using RT-PCR, we found that transcripts of HIBADH are enriched in the cerebellum, heart, skeletal muscle, uterus, placenta, and testes of male humans. In western blotting, it is expressed in the placenta, testes, and spermatozoa. During spermiogenesis, HIBADH is located at the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm. The enzymatic activity of HIBADH in sperm with moderate and lower motility were significantly reduced compared with good motility (P <0.0001 and P <0.05, respectively). Conclusions: Our study indicated that HIBADH is involved in the mitochondrial function of spermatozoa, and maintains sperm motility. It may serve as a sperm-motility marker.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalJournal of Assisted Reproduction and Genetics
Volume30
Issue number4
DOIs
Publication statusAccepted/In press - 2013
Externally publishedYes

Fingerprint

3-hydroxyisobutyrate dehydrogenase
Sperm Motility
Spermatozoa
Proteomics
Placenta
Reverse Transcription
Testis
Asthenozoospermia
Molecular Motor Proteins
Western Blotting
Polymerase Chain Reaction
Male Infertility
Spermatogenesis
Cerebellum
Uterus
Fluorescent Antibody Technique
Myocardium
Mitochondria
Skeletal Muscle
Proteins

Keywords

  • Asthenozoospermia
  • HIBADH
  • Proteomics
  • Sperm motility

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Genetics(clinical)
  • Genetics
  • Developmental Biology

Cite this

Tasi, Y. C., Chao, H. C. A., Chung, C. L., Liu, X. Y., Lin, Y. M., Liao, P. C., ... Lin, Y. H. (Accepted/In press). Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker. Journal of Assisted Reproduction and Genetics, 30(4), 1-8. https://doi.org/10.1007/s10815-013-9954-8

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker. / Tasi, Yung Chieh; Chao, Hsin Chih Albert; Chung, Chia Ling; Liu, Xiu Ying; Lin, Ying Ming; Liao, Pao Chi; Pan, Hsien An; Chiang, Han-Sun; Kuo, Pao Lin; Lin, Ying Hung.

In: Journal of Assisted Reproduction and Genetics, Vol. 30, No. 4, 2013, p. 1-8.

Research output: Contribution to journalArticle

Tasi, YC, Chao, HCA, Chung, CL, Liu, XY, Lin, YM, Liao, PC, Pan, HA, Chiang, H-S, Kuo, PL & Lin, YH 2013, 'Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker', Journal of Assisted Reproduction and Genetics, vol. 30, no. 4, pp. 1-8. https://doi.org/10.1007/s10815-013-9954-8
Tasi, Yung Chieh ; Chao, Hsin Chih Albert ; Chung, Chia Ling ; Liu, Xiu Ying ; Lin, Ying Ming ; Liao, Pao Chi ; Pan, Hsien An ; Chiang, Han-Sun ; Kuo, Pao Lin ; Lin, Ying Hung. / Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker. In: Journal of Assisted Reproduction and Genetics. 2013 ; Vol. 30, No. 4. pp. 1-8.
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abstract = "Purpose: Asthenozoospermia is a major cause of male infertility. However, the molecular mechanisms underlying sperm-motility defects remain largely unknown in the majority of cases. In our previous study, we applied a proteomic approach to identify unknown proteins that were downregulated in spermatozoa with low motility compared to spermatozoa with good motility. Several sperm motility- related proteins have been identified. In this study, 3-hydroxyisobutyrate dehydrogenase (HIBADH), one of the proteins identified using the proteomic tools, is further characterized. Methods: Reverse-transcription polymerase chain reactions (RT-PCR), western blotting, and immunofluorescence assays (IFA) were preformed to investigate the expression pattern. The enzymatic activity of HIBADH was evaluated in sperm with good (>50 {\%}), moderate (<50 {\%}) and lower motility (<20 {\%}). Results: Using RT-PCR, we found that transcripts of HIBADH are enriched in the cerebellum, heart, skeletal muscle, uterus, placenta, and testes of male humans. In western blotting, it is expressed in the placenta, testes, and spermatozoa. During spermiogenesis, HIBADH is located at the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm. The enzymatic activity of HIBADH in sperm with moderate and lower motility were significantly reduced compared with good motility (P <0.0001 and P <0.05, respectively). Conclusions: Our study indicated that HIBADH is involved in the mitochondrial function of spermatozoa, and maintains sperm motility. It may serve as a sperm-motility marker.",
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AU - Tasi, Yung Chieh

AU - Chao, Hsin Chih Albert

AU - Chung, Chia Ling

AU - Liu, Xiu Ying

AU - Lin, Ying Ming

AU - Liao, Pao Chi

AU - Pan, Hsien An

AU - Chiang, Han-Sun

AU - Kuo, Pao Lin

AU - Lin, Ying Hung

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N2 - Purpose: Asthenozoospermia is a major cause of male infertility. However, the molecular mechanisms underlying sperm-motility defects remain largely unknown in the majority of cases. In our previous study, we applied a proteomic approach to identify unknown proteins that were downregulated in spermatozoa with low motility compared to spermatozoa with good motility. Several sperm motility- related proteins have been identified. In this study, 3-hydroxyisobutyrate dehydrogenase (HIBADH), one of the proteins identified using the proteomic tools, is further characterized. Methods: Reverse-transcription polymerase chain reactions (RT-PCR), western blotting, and immunofluorescence assays (IFA) were preformed to investigate the expression pattern. The enzymatic activity of HIBADH was evaluated in sperm with good (>50 %), moderate (<50 %) and lower motility (<20 %). Results: Using RT-PCR, we found that transcripts of HIBADH are enriched in the cerebellum, heart, skeletal muscle, uterus, placenta, and testes of male humans. In western blotting, it is expressed in the placenta, testes, and spermatozoa. During spermiogenesis, HIBADH is located at the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm. The enzymatic activity of HIBADH in sperm with moderate and lower motility were significantly reduced compared with good motility (P <0.0001 and P <0.05, respectively). Conclusions: Our study indicated that HIBADH is involved in the mitochondrial function of spermatozoa, and maintains sperm motility. It may serve as a sperm-motility marker.

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