Characterization of γS-crystallin isoforms from a catfish: Evolutionary comparison of various γ-, γS-, and β-crystallins

Shyh Horng Chiou, Fu Ming Pan, Hsuan Wan Peng, Yen Kai Chao, Wen Chang Chang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

γS-Crystallin from catfish eye lenses, formerly designated βS-crystallin in mammalian lenses, is structurally characterized in this study by cDNA cloning and sequencing. To facilitate sequence characterization of γS-crystallin with structural properties lying between β- and γ-crystallins, a cDNA mixture was constructed from the poly(A)+ mRNA isolated from catfish eye lenses, and amplification by polymerase chain reaction (PCR) was carried out to obtain nucleotide segments encoding multiple γS-crystallin isoforms. Sequencing several positive clones revealed that at least two distinct isoforms exist in the γS-crystallin class of this teleostean fish, similar to the authentic γ-crystallin family characterized previously in species of the piscine class. Comparison of protein sequences encoded by two representative catfish γS1 and γS2 cDNAs with the published sequences of β-, γ-, and γS-crystallins from shark, carp, bullfrog, bovine, and human lenses indicates that there is about 20-50% sequence homology between catfish γS-crystallins and various members of the related β/γ-crystallin superfamily from different evolutionary classes, with a higher sequence similarity being found between catfish γS- and mammalian γ-crystallins than between catfish γS- and bovine or carp γS-crystallins. Phylogenetic trees constructed on the basis of the nucleotide and protein sequence divergence among various β-, γ-, and γS-crystallins corroborate the closer relatedness of catfish γS- to authentic γ-crystallin than to bovine and carp γS-crystallins. The results suggest that evolution of catfish γS-crystallins follows a different path from that of bovine and carp γS-crystallins and may represent a more ancient offshoot from the ancestral γ/γS coding gene than carp and bovine γS-crystallins.

Original languageEnglish
Pages (from-to)412-419
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number2
DOIs
Publication statusPublished - Nov 18 1998
Externally publishedYes

Fingerprint

Catfishes
Crystallins
Protein Isoforms
Carps
Lenses
Crystalline Lens
Complementary DNA
Nucleotides
Sharks
Rana catesbeiana
Cloning

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of γS-crystallin isoforms from a catfish : Evolutionary comparison of various γ-, γS-, and β-crystallins. / Chiou, Shyh Horng; Pan, Fu Ming; Peng, Hsuan Wan; Chao, Yen Kai; Chang, Wen Chang.

In: Biochemical and Biophysical Research Communications, Vol. 252, No. 2, 18.11.1998, p. 412-419.

Research output: Contribution to journalArticle

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abstract = "γS-Crystallin from catfish eye lenses, formerly designated βS-crystallin in mammalian lenses, is structurally characterized in this study by cDNA cloning and sequencing. To facilitate sequence characterization of γS-crystallin with structural properties lying between β- and γ-crystallins, a cDNA mixture was constructed from the poly(A)+ mRNA isolated from catfish eye lenses, and amplification by polymerase chain reaction (PCR) was carried out to obtain nucleotide segments encoding multiple γS-crystallin isoforms. Sequencing several positive clones revealed that at least two distinct isoforms exist in the γS-crystallin class of this teleostean fish, similar to the authentic γ-crystallin family characterized previously in species of the piscine class. Comparison of protein sequences encoded by two representative catfish γS1 and γS2 cDNAs with the published sequences of β-, γ-, and γS-crystallins from shark, carp, bullfrog, bovine, and human lenses indicates that there is about 20-50{\%} sequence homology between catfish γS-crystallins and various members of the related β/γ-crystallin superfamily from different evolutionary classes, with a higher sequence similarity being found between catfish γS- and mammalian γ-crystallins than between catfish γS- and bovine or carp γS-crystallins. Phylogenetic trees constructed on the basis of the nucleotide and protein sequence divergence among various β-, γ-, and γS-crystallins corroborate the closer relatedness of catfish γS- to authentic γ-crystallin than to bovine and carp γS-crystallins. The results suggest that evolution of catfish γS-crystallins follows a different path from that of bovine and carp γS-crystallins and may represent a more ancient offshoot from the ancestral γ/γS coding gene than carp and bovine γS-crystallins.",
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