Characterisation of antibodies to bovine toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2

Lai Shan Kwong, Richard Parsons, Robert Patterson, Tracey J. Coffey, Leenadevi Thonur, Jung Su Chang, George Russell, David Haig, Dirk Werling, Jayne C. Hope

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Host recognition of conserved pathogen-associated molecular patterns (PAMPs) and their interactions with pattern-recognition receptors, including the Toll-like receptors (TLR) is essential for innate immune response induction. The TLR1 family (TLR1, 2, 6 and 10) is involved in the recognition of gram-positive and gram-negative bacteria and heterodimers of TLR1 or TLR6 with TLR2 are crucial for the identification of several PAMPs.Studies on cell surface expression of TLR in ruminants are hampered by the lack of specific antibodies and no convincingly cross-reactive anti-human antibodies have been described so far. We describe herein four antibodies which recognise bovine TLR2. Differences in TLR2 expression were evident on bovine antigen presenting cells with high level expression on peripheral blood monocytes and monocyte-derived macrophages. Lower levels of expression were evident on dendritic cell populations derived in vitro and ex vivo, and on alveolar macrophages. One of the antibodies recognised TLR2 expression on ovine peripheral blood monocytes. The identification of antibodies specific for bovine and ovine TLR2 will facilitate studies of the role of this important PRR in the initiation of immune responses to important pathogens.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalVeterinary Immunology and Immunopathology
Volume139
Issue number2-4
DOIs
Publication statusPublished - Feb 15 2011
Externally publishedYes

Fingerprint

Toll-Like Receptor 2
cross reaction
Sheep
sheep
antibodies
Antibodies
Toll-Like Receptors
monocytes
cattle
Monocytes
Pattern Recognition Receptors
macrophages
pathogen identification
Alveolar Macrophages
Ruminants
Antigen-Presenting Cells
Gram-Negative Bacteria
Innate Immunity
antigen-presenting cells
Dendritic Cells

Keywords

  • Dendritic cell
  • Macrophage
  • Toll-like receptor

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

Characterisation of antibodies to bovine toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2. / Kwong, Lai Shan; Parsons, Richard; Patterson, Robert; Coffey, Tracey J.; Thonur, Leenadevi; Chang, Jung Su; Russell, George; Haig, David; Werling, Dirk; Hope, Jayne C.

In: Veterinary Immunology and Immunopathology, Vol. 139, No. 2-4, 15.02.2011, p. 313-318.

Research output: Contribution to journalArticle

Kwong, LS, Parsons, R, Patterson, R, Coffey, TJ, Thonur, L, Chang, JS, Russell, G, Haig, D, Werling, D & Hope, JC 2011, 'Characterisation of antibodies to bovine toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2', Veterinary Immunology and Immunopathology, vol. 139, no. 2-4, pp. 313-318. https://doi.org/10.1016/j.vetimm.2010.10.014
Kwong, Lai Shan ; Parsons, Richard ; Patterson, Robert ; Coffey, Tracey J. ; Thonur, Leenadevi ; Chang, Jung Su ; Russell, George ; Haig, David ; Werling, Dirk ; Hope, Jayne C. / Characterisation of antibodies to bovine toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2. In: Veterinary Immunology and Immunopathology. 2011 ; Vol. 139, No. 2-4. pp. 313-318.
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