Characterisation of antibodies to bovine toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2

Lai Shan Kwong, Richard Parsons, Robert Patterson, Tracey J. Coffey, Leenadevi Thonur, Jung Su Chang, George Russell, David Haig, Dirk Werling, Jayne C. Hope

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10 Citations (Scopus)

Abstract

Host recognition of conserved pathogen-associated molecular patterns (PAMPs) and their interactions with pattern-recognition receptors, including the Toll-like receptors (TLR) is essential for innate immune response induction. The TLR1 family (TLR1, 2, 6 and 10) is involved in the recognition of gram-positive and gram-negative bacteria and heterodimers of TLR1 or TLR6 with TLR2 are crucial for the identification of several PAMPs.Studies on cell surface expression of TLR in ruminants are hampered by the lack of specific antibodies and no convincingly cross-reactive anti-human antibodies have been described so far. We describe herein four antibodies which recognise bovine TLR2. Differences in TLR2 expression were evident on bovine antigen presenting cells with high level expression on peripheral blood monocytes and monocyte-derived macrophages. Lower levels of expression were evident on dendritic cell populations derived in vitro and ex vivo, and on alveolar macrophages. One of the antibodies recognised TLR2 expression on ovine peripheral blood monocytes. The identification of antibodies specific for bovine and ovine TLR2 will facilitate studies of the role of this important PRR in the initiation of immune responses to important pathogens.

Original languageEnglish
Pages (from-to)313-318
Number of pages6
JournalVeterinary Immunology and Immunopathology
Volume139
Issue number2-4
DOIs
Publication statusPublished - Feb 15 2011
Externally publishedYes

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Keywords

  • Dendritic cell
  • Macrophage
  • Toll-like receptor

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

Kwong, L. S., Parsons, R., Patterson, R., Coffey, T. J., Thonur, L., Chang, J. S., Russell, G., Haig, D., Werling, D., & Hope, J. C. (2011). Characterisation of antibodies to bovine toll-like receptor (TLR)-2 and cross-reactivity with ovine TLR2. Veterinary Immunology and Immunopathology, 139(2-4), 313-318. https://doi.org/10.1016/j.vetimm.2010.10.014