Changes of masseter muscle activity following injection of botulinum toxin type A in adult rats

C. Y. Tsai, Y. Y. Lei, L. Y. Yang, W. C. Chiu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objectives: To investigate changes in masseter muscle function following intramuscular injection of different dose-dependent botulinum toxin type A (BTXA). Setting and Sample Population: Department of Orthodontics at Taipei Medical University. Fifty-two, 70-day-old male Wistar rats were randomly divided into four groups. Group I received 7.5 U of BTXA (0.3 ml), Group II received 5.0 U, and Group III received 2.5 U in the right masseter muscle, respectively. Group IV is the control and received no BTXA injection. Materials and Methods: A wire electrode device was implanted to record muscle activity. One week after implantation, the rats were fed every 2 h and EMG signals were recorded during the first hour. All signals were recorded for 12 weeks. Thereafter, EMG data were analyzed for statistical calculation and weights of masseter muscles were measured. Results: Masseter muscle activity decreased 99% during the first week after BTXA injection and gradually recovered from the 3rd week on in Groups I-III. By the 12th week, muscle activity recovered to 41% in Groups I and II and 56.26% in Group III. No significant changes of muscle activity were observed in Group IV. Conclusion: BTXA induced a reduction in masseter muscle activity and an increased toxin dose resulted in greater depression of muscle activity.

Original languageEnglish
Pages (from-to)202-211
Number of pages10
JournalOrthodontics and Craniofacial Research
Volume18
Issue number4
DOIs
Publication statusPublished - Nov 1 2015

Fingerprint

Masseter Muscle
Type A Botulinum Toxins
Injections
Muscles
Intramuscular Injections
Orthodontics
Wistar Rats
Electrodes
Weights and Measures
Equipment and Supplies
Population

Keywords

  • Botulinum toxin type A
  • EMG
  • Masseter muscle

ASJC Scopus subject areas

  • Orthodontics
  • Oral Surgery
  • Otorhinolaryngology
  • Surgery

Cite this

Changes of masseter muscle activity following injection of botulinum toxin type A in adult rats. / Tsai, C. Y.; Lei, Y. Y.; Yang, L. Y.; Chiu, W. C.

In: Orthodontics and Craniofacial Research, Vol. 18, No. 4, 01.11.2015, p. 202-211.

Research output: Contribution to journalArticle

Tsai, C. Y. ; Lei, Y. Y. ; Yang, L. Y. ; Chiu, W. C. / Changes of masseter muscle activity following injection of botulinum toxin type A in adult rats. In: Orthodontics and Craniofacial Research. 2015 ; Vol. 18, No. 4. pp. 202-211.
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abstract = "Objectives: To investigate changes in masseter muscle function following intramuscular injection of different dose-dependent botulinum toxin type A (BTXA). Setting and Sample Population: Department of Orthodontics at Taipei Medical University. Fifty-two, 70-day-old male Wistar rats were randomly divided into four groups. Group I received 7.5 U of BTXA (0.3 ml), Group II received 5.0 U, and Group III received 2.5 U in the right masseter muscle, respectively. Group IV is the control and received no BTXA injection. Materials and Methods: A wire electrode device was implanted to record muscle activity. One week after implantation, the rats were fed every 2 h and EMG signals were recorded during the first hour. All signals were recorded for 12 weeks. Thereafter, EMG data were analyzed for statistical calculation and weights of masseter muscles were measured. Results: Masseter muscle activity decreased 99{\%} during the first week after BTXA injection and gradually recovered from the 3rd week on in Groups I-III. By the 12th week, muscle activity recovered to 41{\%} in Groups I and II and 56.26{\%} in Group III. No significant changes of muscle activity were observed in Group IV. Conclusion: BTXA induced a reduction in masseter muscle activity and an increased toxin dose resulted in greater depression of muscle activity.",
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