Changes in superoxide dismutase activity and mRNA in vivo after short- term supplementation with trilinolein in rats

Paul Chan, Wen Pin Huang, Ju Chi Liu, Yi Jen Chen, Juei Tang Cheng

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Oxidative damage plays a central role in atherogenesis and antioxidation defense mechanisms may prevent atherosclerosis. This study evaluated the effect of short-term supplementation of the natural lipophilic antioxidant trilinolein on superoxide dismutase (SOD) activity and SOD-mRNA gene expression in vivo in rat vital organs. Methods: Male Wistar rats (n = 8) were injected intraperitoneally with trilinolein (1 mM/ml/kg/day in 0.5% ethanol) daily for three consecutive days. Two control groups (n = 8) were administered saline or 0.5% ethanol in saline, respectively, for three days. Results: Assay of SOD activity and SOD-mRNA by Northern blotting in rat liver, spleen and brain showed significant increases in SOD activity and increased SOD-mRNA gene expression. Conclusions: The natural lipophilic antioxidant trilinolein potentiates the SOD antioxidation defense mechanism and increases gene expression of SOD-mRNA after short-term supplementation in rats.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalChinese Medical Journal (Taipei)
Volume63
Issue number5
Publication statusPublished - May 2000

Fingerprint

Superoxide Dismutase
Messenger RNA
Gene Expression
Atherosclerosis
Ethanol
Antioxidants
trilinolein
Northern Blotting
Wistar Rats
Spleen
Control Groups
Liver
Brain

Keywords

  • Antioxidant
  • Gene expression
  • mRNA
  • Panax pseudoginseng
  • Superoxide dismutase
  • Trilinolein

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Changes in superoxide dismutase activity and mRNA in vivo after short- term supplementation with trilinolein in rats. / Chan, Paul; Huang, Wen Pin; Liu, Ju Chi; Chen, Yi Jen; Cheng, Juei Tang.

In: Chinese Medical Journal (Taipei), Vol. 63, No. 5, 05.2000, p. 355-360.

Research output: Contribution to journalArticle

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AU - Chen, Yi Jen

AU - Cheng, Juei Tang

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N2 - Background: Oxidative damage plays a central role in atherogenesis and antioxidation defense mechanisms may prevent atherosclerosis. This study evaluated the effect of short-term supplementation of the natural lipophilic antioxidant trilinolein on superoxide dismutase (SOD) activity and SOD-mRNA gene expression in vivo in rat vital organs. Methods: Male Wistar rats (n = 8) were injected intraperitoneally with trilinolein (1 mM/ml/kg/day in 0.5% ethanol) daily for three consecutive days. Two control groups (n = 8) were administered saline or 0.5% ethanol in saline, respectively, for three days. Results: Assay of SOD activity and SOD-mRNA by Northern blotting in rat liver, spleen and brain showed significant increases in SOD activity and increased SOD-mRNA gene expression. Conclusions: The natural lipophilic antioxidant trilinolein potentiates the SOD antioxidation defense mechanism and increases gene expression of SOD-mRNA after short-term supplementation in rats.

AB - Background: Oxidative damage plays a central role in atherogenesis and antioxidation defense mechanisms may prevent atherosclerosis. This study evaluated the effect of short-term supplementation of the natural lipophilic antioxidant trilinolein on superoxide dismutase (SOD) activity and SOD-mRNA gene expression in vivo in rat vital organs. Methods: Male Wistar rats (n = 8) were injected intraperitoneally with trilinolein (1 mM/ml/kg/day in 0.5% ethanol) daily for three consecutive days. Two control groups (n = 8) were administered saline or 0.5% ethanol in saline, respectively, for three days. Results: Assay of SOD activity and SOD-mRNA by Northern blotting in rat liver, spleen and brain showed significant increases in SOD activity and increased SOD-mRNA gene expression. Conclusions: The natural lipophilic antioxidant trilinolein potentiates the SOD antioxidation defense mechanism and increases gene expression of SOD-mRNA after short-term supplementation in rats.

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