Changes in corneal basal epithelial phenotypes in an altered basement membrane

I. Jong Wang, Jui-Fang Tsai, Lung Kun Yeh, Ryan Yao Nien Tsai, Fung Rong Hu, Winston W Y Kao

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: To examine the corneal epithelial phenotype in an altered basement membrane. Methodology/Principal Findings: Corneas from 9 patients with symptoms of continuous unstable corneal curvature (CUCC) were harvested by penetrating keratoplasty and subjected to histology examination and immunohistochemical staining with transactivating and N-terminally truncated pP63 transcript (DNp63), cytokeratin 3 (Krt3), ATP-binding cassette subfamily G member 2 (ABCG2), connexin 43 (CX43), p38 mitogen-activated protein kinases (p38MAPK), activating protein 2 (TFAP2), and extracellular signal-regulated kinase (Erk1/2) monoclonal antibodies. Positive immunostaining with ABCG2, p38MAPK, and TFAP2 monoclonal antibodies was observed in the basal epithelial cells of CUCC patients, and CX43 and DNp63 were detected in the full-thickness epithelial cells of CUCC patients. Conclusions/Significance: Our results indicate that alteration of the corneal basement membrane induces a dedifferentiation- like phenotype in corneal basal epithelial cells.

Original languageEnglish
Article numbere14537
JournalPLoS One
Volume6
Issue number1
DOIs
Publication statusPublished - 2011

Fingerprint

basement membrane
mitogen-activated protein kinase
Basement Membrane
connexins
Connexin 43
epithelial cells
Epithelial Cells
p38 Mitogen-Activated Protein Kinases
Phenotype
phenotype
monoclonal antibodies
Keratin-3
Adenosine Triphosphate
Monoclonal Antibodies
Penetrating Keratoplasty
Histology
Mitogen-Activated Protein Kinase 1
cornea
Cornea
histology

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Wang, I. J., Tsai, J-F., Yeh, L. K., Tsai, R. Y. N., Hu, F. R., & Kao, W. W. Y. (2011). Changes in corneal basal epithelial phenotypes in an altered basement membrane. PLoS One, 6(1), [e14537]. https://doi.org/10.1371/journal.pone.0014537

Changes in corneal basal epithelial phenotypes in an altered basement membrane. / Wang, I. Jong; Tsai, Jui-Fang; Yeh, Lung Kun; Tsai, Ryan Yao Nien; Hu, Fung Rong; Kao, Winston W Y.

In: PLoS One, Vol. 6, No. 1, e14537, 2011.

Research output: Contribution to journalArticle

Wang, IJ, Tsai, J-F, Yeh, LK, Tsai, RYN, Hu, FR & Kao, WWY 2011, 'Changes in corneal basal epithelial phenotypes in an altered basement membrane', PLoS One, vol. 6, no. 1, e14537. https://doi.org/10.1371/journal.pone.0014537
Wang, I. Jong ; Tsai, Jui-Fang ; Yeh, Lung Kun ; Tsai, Ryan Yao Nien ; Hu, Fung Rong ; Kao, Winston W Y. / Changes in corneal basal epithelial phenotypes in an altered basement membrane. In: PLoS One. 2011 ; Vol. 6, No. 1.
@article{2118de4658bc49c5bf90b59e1f742116,
title = "Changes in corneal basal epithelial phenotypes in an altered basement membrane",
abstract = "Background: To examine the corneal epithelial phenotype in an altered basement membrane. Methodology/Principal Findings: Corneas from 9 patients with symptoms of continuous unstable corneal curvature (CUCC) were harvested by penetrating keratoplasty and subjected to histology examination and immunohistochemical staining with transactivating and N-terminally truncated pP63 transcript (DNp63), cytokeratin 3 (Krt3), ATP-binding cassette subfamily G member 2 (ABCG2), connexin 43 (CX43), p38 mitogen-activated protein kinases (p38MAPK), activating protein 2 (TFAP2), and extracellular signal-regulated kinase (Erk1/2) monoclonal antibodies. Positive immunostaining with ABCG2, p38MAPK, and TFAP2 monoclonal antibodies was observed in the basal epithelial cells of CUCC patients, and CX43 and DNp63 were detected in the full-thickness epithelial cells of CUCC patients. Conclusions/Significance: Our results indicate that alteration of the corneal basement membrane induces a dedifferentiation- like phenotype in corneal basal epithelial cells.",
author = "Wang, {I. Jong} and Jui-Fang Tsai and Yeh, {Lung Kun} and Tsai, {Ryan Yao Nien} and Hu, {Fung Rong} and Kao, {Winston W Y}",
year = "2011",
doi = "10.1371/journal.pone.0014537",
language = "English",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "1",

}

TY - JOUR

T1 - Changes in corneal basal epithelial phenotypes in an altered basement membrane

AU - Wang, I. Jong

AU - Tsai, Jui-Fang

AU - Yeh, Lung Kun

AU - Tsai, Ryan Yao Nien

AU - Hu, Fung Rong

AU - Kao, Winston W Y

PY - 2011

Y1 - 2011

N2 - Background: To examine the corneal epithelial phenotype in an altered basement membrane. Methodology/Principal Findings: Corneas from 9 patients with symptoms of continuous unstable corneal curvature (CUCC) were harvested by penetrating keratoplasty and subjected to histology examination and immunohistochemical staining with transactivating and N-terminally truncated pP63 transcript (DNp63), cytokeratin 3 (Krt3), ATP-binding cassette subfamily G member 2 (ABCG2), connexin 43 (CX43), p38 mitogen-activated protein kinases (p38MAPK), activating protein 2 (TFAP2), and extracellular signal-regulated kinase (Erk1/2) monoclonal antibodies. Positive immunostaining with ABCG2, p38MAPK, and TFAP2 monoclonal antibodies was observed in the basal epithelial cells of CUCC patients, and CX43 and DNp63 were detected in the full-thickness epithelial cells of CUCC patients. Conclusions/Significance: Our results indicate that alteration of the corneal basement membrane induces a dedifferentiation- like phenotype in corneal basal epithelial cells.

AB - Background: To examine the corneal epithelial phenotype in an altered basement membrane. Methodology/Principal Findings: Corneas from 9 patients with symptoms of continuous unstable corneal curvature (CUCC) were harvested by penetrating keratoplasty and subjected to histology examination and immunohistochemical staining with transactivating and N-terminally truncated pP63 transcript (DNp63), cytokeratin 3 (Krt3), ATP-binding cassette subfamily G member 2 (ABCG2), connexin 43 (CX43), p38 mitogen-activated protein kinases (p38MAPK), activating protein 2 (TFAP2), and extracellular signal-regulated kinase (Erk1/2) monoclonal antibodies. Positive immunostaining with ABCG2, p38MAPK, and TFAP2 monoclonal antibodies was observed in the basal epithelial cells of CUCC patients, and CX43 and DNp63 were detected in the full-thickness epithelial cells of CUCC patients. Conclusions/Significance: Our results indicate that alteration of the corneal basement membrane induces a dedifferentiation- like phenotype in corneal basal epithelial cells.

UR - http://www.scopus.com/inward/record.url?scp=79251578346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251578346&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0014537

DO - 10.1371/journal.pone.0014537

M3 - Article

C2 - 21264285

AN - SCOPUS:79251578346

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 1

M1 - e14537

ER -