Changes in corneal basal epithelial phenotypes in an altered basement membrane

I. Jong Wang, Jui-Fang Tsai, Lung Kun Yeh, Ryan Yao Nien Tsai, Fung Rong Hu, Winston W Y Kao

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: To examine the corneal epithelial phenotype in an altered basement membrane. Methodology/Principal Findings: Corneas from 9 patients with symptoms of continuous unstable corneal curvature (CUCC) were harvested by penetrating keratoplasty and subjected to histology examination and immunohistochemical staining with transactivating and N-terminally truncated pP63 transcript (DNp63), cytokeratin 3 (Krt3), ATP-binding cassette subfamily G member 2 (ABCG2), connexin 43 (CX43), p38 mitogen-activated protein kinases (p38MAPK), activating protein 2 (TFAP2), and extracellular signal-regulated kinase (Erk1/2) monoclonal antibodies. Positive immunostaining with ABCG2, p38MAPK, and TFAP2 monoclonal antibodies was observed in the basal epithelial cells of CUCC patients, and CX43 and DNp63 were detected in the full-thickness epithelial cells of CUCC patients. Conclusions/Significance: Our results indicate that alteration of the corneal basement membrane induces a dedifferentiation- like phenotype in corneal basal epithelial cells.

Original languageEnglish
Article numbere14537
JournalPLoS One
Volume6
Issue number1
DOIs
Publication statusPublished - 2011

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Wang, I. J., Tsai, J-F., Yeh, L. K., Tsai, R. Y. N., Hu, F. R., & Kao, W. W. Y. (2011). Changes in corneal basal epithelial phenotypes in an altered basement membrane. PLoS One, 6(1), [e14537]. https://doi.org/10.1371/journal.pone.0014537