Change of serum vascular adhesion protein-1 after glucose loading correlates to carotid intima-medial thickness in non-diabetic subjects

Hung Yuan Li, Mao Shin Lin, Jung Nan Wei, Chi Sheng Hung, Fu Tien Chiang, Cheng Hsin Lin, Hsiu Ching Hsu, Chien Yin Su, Mei Yu Wu, David J. Smith, Jani Vainio, Ming Fong Chen, Lee Ming Chuang

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Background: We investigated if serum vascular adhesion protein-1 (SSAO/VAP-1) changed acutely following oral glucose loading and whether such changes are correlated with surrogate markers of atherosclerosis. Methods: A total of 115 non-diabetics subjects were enrolled for an oral glucose tolerance test (OGTT). Carotid intima-medial thickness (IMT) was measured by ultrasonography. Serum SSAO/VAP-1 was analyzed by time-resolved immunofluorometric assay. Serum thiobarbituric acid reactive substances (TBARS) and advanced glycated end products (AGEs) were measured by fluorometric assays. Results: Serum SSAO/VAP-1 increased significantly at 30 min after oral glucose loading and lasted to 2 h (p = 0.0005 and p <0.0001, for 30 min and 2 h respectively). The area under curve of serum SSAO/VAP-1 during OGTT (AUC-VAP-1) correlated significantly with carotid IMT, independent of age, gender, low-density lipoprotein cholesterol, systolic blood pressure, hemoglobin A1c, serum TBARS, AGEs, and high-sensitivity C-reactive protein. Subjects with a positive AUC-VAP-1 had significantly higher serum TBARS and AGEs than subjects with a negative AUC-VAP-1 adjusted for age and gender. Conclusions: Serum SSAO/VAP-1 changed acutely following oral glucose loading in non-diabetic subjects. Change of serum SSAO/VAP-1 correlated independently to serum TBARS, AGEs, and carotid IMT. Our findings suggest that acute change of serum SSAO/VAP-1 is a novel marker for hyperglycemia-induced atherosclerosis.

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalClinica Chimica Acta
Volume403
Issue number1-2
DOIs
Publication statusPublished - May 2009
Externally publishedYes

Keywords

  • Advanced glycated end products
  • Carotid intima-medial thickness
  • Oxidative stress
  • Vascular adhesion protein-1

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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