Change in centromeric and acentromeric micronucleus frequencies in human populations after chronic radiation exposure

Wushou P. Chang, Wanhua A. Hsieh, Ding Ping Chen, Yi Ping Lin, Jing Shiang Hwang, Jeng Jong J Hwang, Mong Hsun Tsai, Bing Fang Hwang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Acute radiation exposure of humans was observed to induce various forms of cytogenetic damage, including increased frequencies of micronuclei and chromosomal aberrations. However, the cytogenetic effects of chronic low dose radiation exposure in vivo needs further characterization. Sixteen subjects with chronic low dose rates of γ-radiation exposure from 60Co-contaminated steel in radioactive buildings were compared with seven non-exposed reference subjects for micronucleus frequencies after they relocated. By in situ hybridization using a digoxigenin-labeled anti-α all human centromere probe, the exposed subjects were shown to have a significant increase in cytochalasin B-modulated micronucleus (CBMN) frequencies, as well as a significant increase in centromere-positive (C+) CBMN, centromere-negative (C-) CBMN, total C+ signals, single C+ MN signals and multiple C+ signals/1000 binucleated cells (BN). However, decreases in the ratios C+ MN/C- MN and C+ MN/total CBMN (%) were also noted in the exposed subjects. By mixed effects analysis, considering individuals from the same families, the C- MN and single C+ MN/1000 BN were both positively and moderately associated with previous cumulative exposure. When the time period of relocation post-exposure (relocation time or RT) was considered, total C+ MN and multiple C+ MN/1000 BN were negatively and significantly associated with RT. Moreover, the C+ MN, C- MN, CS MN/C- MN ratio and single C+ MN/1000 BN were all negatively and moderately associated with RT, but not with exposure dose. This suggested that acentromeric and single or multiple centromeric CBMN cytogenetic damage seems to disappear differentially in human subjects post chronic low dose radiation exposure.

Original languageEnglish
Pages (from-to)427-432
Number of pages6
JournalMutagenesis
Volume14
Issue number4
DOIs
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Cytochalasin B
Centromere
Radiation
Cytogenetics
Dosimetry
Relocation
Population
Digoxigenin
Steel
Aberrations
Chromosome Aberrations
In Situ Hybridization
Radiation Exposure

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Chang, W. P., Hsieh, W. A., Chen, D. P., Lin, Y. P., Hwang, J. S., Hwang, J. J. J., ... Hwang, B. F. (1999). Change in centromeric and acentromeric micronucleus frequencies in human populations after chronic radiation exposure. Mutagenesis, 14(4), 427-432. https://doi.org/10.1093/mutage/14.4.427

Change in centromeric and acentromeric micronucleus frequencies in human populations after chronic radiation exposure. / Chang, Wushou P.; Hsieh, Wanhua A.; Chen, Ding Ping; Lin, Yi Ping; Hwang, Jing Shiang; Hwang, Jeng Jong J; Tsai, Mong Hsun; Hwang, Bing Fang.

In: Mutagenesis, Vol. 14, No. 4, 1999, p. 427-432.

Research output: Contribution to journalArticle

Chang, WP, Hsieh, WA, Chen, DP, Lin, YP, Hwang, JS, Hwang, JJJ, Tsai, MH & Hwang, BF 1999, 'Change in centromeric and acentromeric micronucleus frequencies in human populations after chronic radiation exposure', Mutagenesis, vol. 14, no. 4, pp. 427-432. https://doi.org/10.1093/mutage/14.4.427
Chang, Wushou P. ; Hsieh, Wanhua A. ; Chen, Ding Ping ; Lin, Yi Ping ; Hwang, Jing Shiang ; Hwang, Jeng Jong J ; Tsai, Mong Hsun ; Hwang, Bing Fang. / Change in centromeric and acentromeric micronucleus frequencies in human populations after chronic radiation exposure. In: Mutagenesis. 1999 ; Vol. 14, No. 4. pp. 427-432.
@article{ebc0b591248a4864af3025243e2ecfa2,
title = "Change in centromeric and acentromeric micronucleus frequencies in human populations after chronic radiation exposure",
abstract = "Acute radiation exposure of humans was observed to induce various forms of cytogenetic damage, including increased frequencies of micronuclei and chromosomal aberrations. However, the cytogenetic effects of chronic low dose radiation exposure in vivo needs further characterization. Sixteen subjects with chronic low dose rates of γ-radiation exposure from 60Co-contaminated steel in radioactive buildings were compared with seven non-exposed reference subjects for micronucleus frequencies after they relocated. By in situ hybridization using a digoxigenin-labeled anti-α all human centromere probe, the exposed subjects were shown to have a significant increase in cytochalasin B-modulated micronucleus (CBMN) frequencies, as well as a significant increase in centromere-positive (C+) CBMN, centromere-negative (C-) CBMN, total C+ signals, single C+ MN signals and multiple C+ signals/1000 binucleated cells (BN). However, decreases in the ratios C+ MN/C- MN and C+ MN/total CBMN ({\%}) were also noted in the exposed subjects. By mixed effects analysis, considering individuals from the same families, the C- MN and single C+ MN/1000 BN were both positively and moderately associated with previous cumulative exposure. When the time period of relocation post-exposure (relocation time or RT) was considered, total C+ MN and multiple C+ MN/1000 BN were negatively and significantly associated with RT. Moreover, the C+ MN, C- MN, CS MN/C- MN ratio and single C+ MN/1000 BN were all negatively and moderately associated with RT, but not with exposure dose. This suggested that acentromeric and single or multiple centromeric CBMN cytogenetic damage seems to disappear differentially in human subjects post chronic low dose radiation exposure.",
author = "Chang, {Wushou P.} and Hsieh, {Wanhua A.} and Chen, {Ding Ping} and Lin, {Yi Ping} and Hwang, {Jing Shiang} and Hwang, {Jeng Jong J} and Tsai, {Mong Hsun} and Hwang, {Bing Fang}",
year = "1999",
doi = "10.1093/mutage/14.4.427",
language = "English",
volume = "14",
pages = "427--432",
journal = "Mutagenesis",
issn = "0267-8357",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Change in centromeric and acentromeric micronucleus frequencies in human populations after chronic radiation exposure

AU - Chang, Wushou P.

AU - Hsieh, Wanhua A.

AU - Chen, Ding Ping

AU - Lin, Yi Ping

AU - Hwang, Jing Shiang

AU - Hwang, Jeng Jong J

AU - Tsai, Mong Hsun

AU - Hwang, Bing Fang

PY - 1999

Y1 - 1999

N2 - Acute radiation exposure of humans was observed to induce various forms of cytogenetic damage, including increased frequencies of micronuclei and chromosomal aberrations. However, the cytogenetic effects of chronic low dose radiation exposure in vivo needs further characterization. Sixteen subjects with chronic low dose rates of γ-radiation exposure from 60Co-contaminated steel in radioactive buildings were compared with seven non-exposed reference subjects for micronucleus frequencies after they relocated. By in situ hybridization using a digoxigenin-labeled anti-α all human centromere probe, the exposed subjects were shown to have a significant increase in cytochalasin B-modulated micronucleus (CBMN) frequencies, as well as a significant increase in centromere-positive (C+) CBMN, centromere-negative (C-) CBMN, total C+ signals, single C+ MN signals and multiple C+ signals/1000 binucleated cells (BN). However, decreases in the ratios C+ MN/C- MN and C+ MN/total CBMN (%) were also noted in the exposed subjects. By mixed effects analysis, considering individuals from the same families, the C- MN and single C+ MN/1000 BN were both positively and moderately associated with previous cumulative exposure. When the time period of relocation post-exposure (relocation time or RT) was considered, total C+ MN and multiple C+ MN/1000 BN were negatively and significantly associated with RT. Moreover, the C+ MN, C- MN, CS MN/C- MN ratio and single C+ MN/1000 BN were all negatively and moderately associated with RT, but not with exposure dose. This suggested that acentromeric and single or multiple centromeric CBMN cytogenetic damage seems to disappear differentially in human subjects post chronic low dose radiation exposure.

AB - Acute radiation exposure of humans was observed to induce various forms of cytogenetic damage, including increased frequencies of micronuclei and chromosomal aberrations. However, the cytogenetic effects of chronic low dose radiation exposure in vivo needs further characterization. Sixteen subjects with chronic low dose rates of γ-radiation exposure from 60Co-contaminated steel in radioactive buildings were compared with seven non-exposed reference subjects for micronucleus frequencies after they relocated. By in situ hybridization using a digoxigenin-labeled anti-α all human centromere probe, the exposed subjects were shown to have a significant increase in cytochalasin B-modulated micronucleus (CBMN) frequencies, as well as a significant increase in centromere-positive (C+) CBMN, centromere-negative (C-) CBMN, total C+ signals, single C+ MN signals and multiple C+ signals/1000 binucleated cells (BN). However, decreases in the ratios C+ MN/C- MN and C+ MN/total CBMN (%) were also noted in the exposed subjects. By mixed effects analysis, considering individuals from the same families, the C- MN and single C+ MN/1000 BN were both positively and moderately associated with previous cumulative exposure. When the time period of relocation post-exposure (relocation time or RT) was considered, total C+ MN and multiple C+ MN/1000 BN were negatively and significantly associated with RT. Moreover, the C+ MN, C- MN, CS MN/C- MN ratio and single C+ MN/1000 BN were all negatively and moderately associated with RT, but not with exposure dose. This suggested that acentromeric and single or multiple centromeric CBMN cytogenetic damage seems to disappear differentially in human subjects post chronic low dose radiation exposure.

UR - http://www.scopus.com/inward/record.url?scp=0032764131&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032764131&partnerID=8YFLogxK

U2 - 10.1093/mutage/14.4.427

DO - 10.1093/mutage/14.4.427

M3 - Article

C2 - 10390511

AN - SCOPUS:0032764131

VL - 14

SP - 427

EP - 432

JO - Mutagenesis

JF - Mutagenesis

SN - 0267-8357

IS - 4

ER -