Ceramide synthase 6 predicts the prognosis of human gastric cancer

It functions as an oncoprotein by dysregulating the SOCS2/JAK2/STAT3 pathway

Yih Huei Uen, Chia Lang Fang, Chih Chan Lin, You Cheng Hseu, Shih Ting Hung, Ding Ping Sun, Kai Yuan Lin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Although gastric cancer (GC) is one of the most common cancers, knowledge of its development, and carcinogenesis is limited. The present study explored the involvement of ceramide synthase 6 (CERS6) in GC carcinogenesis and prognosis. RT-PCR, immunoblotting, and immunohistochemistry were used to examine the expression of CERS6. Transfection and small hairpin RNA technology were used to investigate the effect of CERS6 manipulation on cell proliferation and spread as well as the underlying mechanism. Moreover, xenograft proliferation was employed to explore the influence of CERS6 on tumor growth in animals. It was found that overexpression of CERS6 was significantly correlated with several clinicopathologic parameters and poor disease-free survival. The overexpression and silencing of CERS6 in GC cells facilitated and suppressed cell proliferation and spread as well as xenograft proliferation, respectively. Mechanistic studies further revealed that CERS6 influenced cell proliferation and spread by regulating cell cycle control and metastasis-related protein through the SOCS2/JAK2/STAT3 signaling pathway. Collectively, this study suggests that CERS6 overexpression could be a useful biomarker for predicting the outcomes of GC patients and that CERS6 targeting represents a potential modality for treating GC.

Original languageEnglish
JournalMolecular Carcinogenesis
DOIs
Publication statusAccepted/In press - Jan 1 2018

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Oncogene Proteins
Stomach Neoplasms
Cell Proliferation
Heterografts
Carcinogenesis
N-(alpha-hydroxyoctadecanoyl)phytosphingosine
dihydroceramide desaturase
Cell Cycle Checkpoints
Immunoblotting
Small Interfering RNA
Disease-Free Survival
Transfection
Neoplasms
Biomarkers
Immunohistochemistry
Neoplasm Metastasis
Technology
Polymerase Chain Reaction
Growth

Keywords

  • CERS6
  • gastric cancer
  • immunohistochemistry
  • prognosis
  • SOCS2

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

Ceramide synthase 6 predicts the prognosis of human gastric cancer : It functions as an oncoprotein by dysregulating the SOCS2/JAK2/STAT3 pathway. / Uen, Yih Huei; Fang, Chia Lang; Lin, Chih Chan; Hseu, You Cheng; Hung, Shih Ting; Sun, Ding Ping; Lin, Kai Yuan.

In: Molecular Carcinogenesis, 01.01.2018.

Research output: Contribution to journalArticle

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