Ceramide and toll-like receptor 4 are mobilized into membrane rafts in response to Helicobacter pylori infection in gastric epithelial cells

Dah Yuu Lu, Hui Chen Chen, Mei Shiang Yang, Yuan Man Hsu, Hwai Jeng Lin, Chih Hsin Tang, Che Hsin Lee, Cheng Kuo Lai, Chun Jung Lin, Woei Cherng Shyu, Fong Yan Lin, Chih Ho Lai

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Abstract

Helicobacter pylori infection is thought to be involved in the development of several gastric diseases. Two H. pylori virulence factors (vacuolating cytotoxin A and cytotoxin-associated gene A) reportedly interact with lipid rafts in gastric epithelial cells. The role of Toll-like receptor (TLR)-mediated signaling in response to H. pylori infection has been investigated extensively in host cells. However, the receptor molecules in lipid rafts that are involved in H. pylori-induced innate sensing have not been well characterized. This study investigated whether lipid rafts play a role in H. pylori-induced ceramide secretion and TLR4 expression and thereby contribute to inflammation in gastric epithelial cells. We observed that both TLR4 and MD-2 mRNA and protein levels were significantly higher in H. pylori-infected AGS cells than in mock-infected cells. Moreover, significantly more TLR4 protein was detected in detergent-resistant membranes extracted from H. pylori-infected AGS cells than in those extracted from mock-infected cells. However, this effect was attenuated by the treatment of cells with cholesterol-usurping agents, suggesting that H. pylori-induced TLR4 signaling is dependent on cholesterol-rich microdomains. Similarly, the level of cellular ceramide was elevated and ceramide was translocated into lipid rafts after H. pylori infection, leading to interleukin-8 (IL-8) production. Using the sphingomyelinase inhibitor imipramine, we observed that H. pylori-induced TLR4 expression was ceramide dependent. These results indicate the mobilization of ceramide and TLR4 into lipid rafts by H. pylori infection in response to inflammation in gastric epithelial cells.

Original languageEnglish
Pages (from-to)1823-1833
Number of pages11
JournalInfection and Immunity
Volume80
Issue number5
DOIs
Publication statusPublished - May 2012

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Toll-Like Receptor 4
Ceramides
Helicobacter Infections
Helicobacter pylori
Stomach
Epithelial Cells
Membranes
Lipids
Cytotoxins
Lymphocyte Antigen 96
Cholesterol
Inflammation
Sphingomyelin Phosphodiesterase
Stomach Diseases
Imipramine
Toll-Like Receptors
Virulence Factors
Interleukin-8
Detergents

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Ceramide and toll-like receptor 4 are mobilized into membrane rafts in response to Helicobacter pylori infection in gastric epithelial cells. / Lu, Dah Yuu; Chen, Hui Chen; Yang, Mei Shiang; Hsu, Yuan Man; Lin, Hwai Jeng; Tang, Chih Hsin; Lee, Che Hsin; Lai, Cheng Kuo; Lin, Chun Jung; Shyu, Woei Cherng; Lin, Fong Yan; Lai, Chih Ho.

In: Infection and Immunity, Vol. 80, No. 5, 05.2012, p. 1823-1833.

Research output: Contribution to journalArticle

Lu, DY, Chen, HC, Yang, MS, Hsu, YM, Lin, HJ, Tang, CH, Lee, CH, Lai, CK, Lin, CJ, Shyu, WC, Lin, FY & Lai, CH 2012, 'Ceramide and toll-like receptor 4 are mobilized into membrane rafts in response to Helicobacter pylori infection in gastric epithelial cells', Infection and Immunity, vol. 80, no. 5, pp. 1823-1833. https://doi.org/10.1128/IAI.05856-11
Lu, Dah Yuu ; Chen, Hui Chen ; Yang, Mei Shiang ; Hsu, Yuan Man ; Lin, Hwai Jeng ; Tang, Chih Hsin ; Lee, Che Hsin ; Lai, Cheng Kuo ; Lin, Chun Jung ; Shyu, Woei Cherng ; Lin, Fong Yan ; Lai, Chih Ho. / Ceramide and toll-like receptor 4 are mobilized into membrane rafts in response to Helicobacter pylori infection in gastric epithelial cells. In: Infection and Immunity. 2012 ; Vol. 80, No. 5. pp. 1823-1833.
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abstract = "Helicobacter pylori infection is thought to be involved in the development of several gastric diseases. Two H. pylori virulence factors (vacuolating cytotoxin A and cytotoxin-associated gene A) reportedly interact with lipid rafts in gastric epithelial cells. The role of Toll-like receptor (TLR)-mediated signaling in response to H. pylori infection has been investigated extensively in host cells. However, the receptor molecules in lipid rafts that are involved in H. pylori-induced innate sensing have not been well characterized. This study investigated whether lipid rafts play a role in H. pylori-induced ceramide secretion and TLR4 expression and thereby contribute to inflammation in gastric epithelial cells. We observed that both TLR4 and MD-2 mRNA and protein levels were significantly higher in H. pylori-infected AGS cells than in mock-infected cells. Moreover, significantly more TLR4 protein was detected in detergent-resistant membranes extracted from H. pylori-infected AGS cells than in those extracted from mock-infected cells. However, this effect was attenuated by the treatment of cells with cholesterol-usurping agents, suggesting that H. pylori-induced TLR4 signaling is dependent on cholesterol-rich microdomains. Similarly, the level of cellular ceramide was elevated and ceramide was translocated into lipid rafts after H. pylori infection, leading to interleukin-8 (IL-8) production. Using the sphingomyelinase inhibitor imipramine, we observed that H. pylori-induced TLR4 expression was ceramide dependent. These results indicate the mobilization of ceramide and TLR4 into lipid rafts by H. pylori infection in response to inflammation in gastric epithelial cells.",
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AU - Hsu, Yuan Man

AU - Lin, Hwai Jeng

AU - Tang, Chih Hsin

AU - Lee, Che Hsin

AU - Lai, Cheng Kuo

AU - Lin, Chun Jung

AU - Shyu, Woei Cherng

AU - Lin, Fong Yan

AU - Lai, Chih Ho

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