Cellular response of calcium phosphate bone substitute containing hydroxyapatite and tricalcium phosphate

Chung Lung Wu, Shih Fu Ou, Ta Sen Huang, Tzu Sen Yang, Mao Sheng Wang, Keng Liang Ou

Research output: Contribution to journalArticle

2 Citations (Scopus)


PURPOSE: This study developed calcium phosphate bone substitutes and their microstucture and in vitro cell response were evaluated in comparison with commercial hydroxyapatite (HA). MATERIALS: HA powder was ball-milled and then sintered to transfer into the calcium phosphate bulks (CPB). The density, hardness, and microstructure of the CPB were investigated. The viability and proliferation of MG63 osteoblast-like cells on the commercial HA and the CPB were evaluated. RESULTS: The x-ray diffraction confirmed that the CPB consisted of α-tricalcium phosphate (α-TCP), CaO, and HA. The hardness, density, and α-TCP-to-HA ratio of the CPB decreased when increasing the sintering duration. Cell tests demonstrated that the CPB exhibited an earlier cell-spread response than the commercial HA. CONCLUSIONS: This study demonstrated that a phase transformation of HA into α-TCP and CaO was achieved by sintering. The cell tests indicated that the CPB has favorable in vitro cellular performance, which implied that it presented potential as bone substitute.

Original languageEnglish
Pages (from-to)74-78
Number of pages5
JournalImplant Dentistry
Issue number1
Publication statusPublished - Feb 2014



  • hydroxyapatite
  • in vitro
  • substitute

ASJC Scopus subject areas

  • Oral Surgery
  • Medicine(all)

Cite this